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The MPM-2 antibody inhibits mitogen-activated protein kinase activity by binding to an epitope containing phosphothreonine-183.
Taagepera S, Dent P, Her JH, Sturgill TW, Gorbsky GJ.
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Mitogen-activated protein (MAP) kinases are a family of serine/threonine kinases implicated in the control of cell proliferation and differentiation. We have found that activated p42mapk is a target for the phosphoepitope antibody MPM-2, a monoclonal antibody that recognizes a cell cycle-regulated phosphoepitope. We have determined that the MPM-2 antibody recognizes the regulatory region of p42mapk. Binding of the MPM-2 antibody to active p42mapk in vitro results in a decrease in p42mapk enzymatic activity. The MPM-2 phosphoepitope can be generated in vitro on bacterially expressed p42mapk by phosphorylation with either isoform of MAP kinase kinase (MKK), MKK1, or MKK2. Analysis of p42mapk proteins mutated in their regulatory sites shows that phosphorylated Thr-183 is essential for the binding of the MPM-2 antibody. MPM-2 binding to Thr-183 is affected by the amino acid present in the other regulatory site, Tyr-185. Substitution of Tyr-185 with phenylalanine results in strong binding of the MPM-2 antibody, whereas substitution with glutamic acid substantially diminishes MPM-2 antibody binding. The MPM-2 phosphoepitope antibody recognizes an amino acid domain incorporating the regulatory phosphothreonine on activated p42mapk in eggs during meiosis and in mammalian cultured cells during the G0 to G1 transition.
Ahn,
The mitogen-activated protein kinase activator.
1992, Pubmed
Ahn,
The mitogen-activated protein kinase activator.
1992,
Pubmed Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed Barrett,
A purified S6 kinase kinase from Xenopus eggs activates S6 kinase II and autophosphorylates on serine, threonine, and tyrosine residues.
1992,
Pubmed
,
Xenbase Crews,
Extracellular signals and reversible protein phosphorylation: what to Mek of it all.
1993,
Pubmed Davis,
Monoclonal antibodies to mitotic cells.
1983,
Pubmed
,
Xenbase Dent,
Expression, purification and characterization of recombinant mitogen-activated protein kinase kinases.
1994,
Pubmed Erickson,
Identification by mass spectrometry of threonine 97 in bovine myelin basic protein as a specific phosphorylation site for mitogen-activated protein kinase.
1990,
Pubmed Errede,
A conserved kinase cascade for MAP kinase activation in yeast.
1993,
Pubmed Fabian,
Critical tyrosine residues regulate the enzymatic and biological activity of Raf-1 kinase.
1993,
Pubmed
,
Xenbase Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Haccard,
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
1993,
Pubmed
,
Xenbase Haccard,
In vivo activation of a microtubule-associated protein kinase during meiotic maturation of the Xenopus oocyte.
1990,
Pubmed
,
Xenbase Haystead,
Ordered phosphorylation of p42mapk by MAP kinase kinase.
1992,
Pubmed Her,
Dual phosphorylation and autophosphorylation in mitogen-activated protein (MAP) kinase activation.
1993,
Pubmed Kuang,
At least two kinases phosphorylate the MPM-2 epitope during Xenopus oocyte maturation.
1993,
Pubmed
,
Xenbase Kuang,
Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.
1989,
Pubmed
,
Xenbase Kuang,
cdc25 is one of the MPM-2 antigens involved in the activation of maturation-promoting factor.
1994,
Pubmed
,
Xenbase Mazzoni,
The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae.
1993,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Payne,
Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).
1991,
Pubmed Pelech,
Mitogen-activated protein kinases: versatile transducers for cell signaling.
1992,
Pubmed Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Ruderman,
MAP kinase and the activation of quiescent cells.
1993,
Pubmed Seger,
Purification and characterization of mitogen-activated protein kinase activator(s) from epidermal growth factor-stimulated A431 cells.
1992,
Pubmed
,
Xenbase Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
,
Xenbase Taagepera,
DNA topoisomerase II alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2.
1993,
Pubmed Tamemoto,
Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells.
1992,
Pubmed Tombes,
Specific association of an M-phase kinase with isolated mitotic spindles and identification of two of its substrates as MAP4 and MAP1B.
1991,
Pubmed Vandre,
Phosphoproteins are components of mitotic microtubule organizing centers.
1984,
Pubmed Vandré,
Proteins of the mammalian mitotic spindle: phosphorylation/dephosphorylation of MAP-4 during mitosis.
1991,
Pubmed Verlhac,
Microtubule and chromatin behavior follow MAP kinase activity but not MPF activity during meiosis in mouse oocytes.
1994,
Pubmed
,
Xenbase Westendorf,
Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.
1994,
Pubmed Wu,
Apparent sufficiency of a dual-specificity tyrosine/threonine kinase for activation of MAP kinase poses new questions for the dual-phosphorylation mechanism.
1992,
Pubmed Zhao,
Threonine phosphorylation is associated with mitosis in HeLa cells.
1989,
Pubmed
