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The transcription factor E2F has been implicated in cell cycle control by virtue of its association with cyclins, cyclin-dependent kinases, and pRb-related tumor suppressor gene products. Eggs and embryos from the frog Xenopus laevis have been used to investigate the characteristics of E2F-like molecules in the Xenopus cell cycle and throughout early development. We find multiple E2F species in Xenopus eggs, at least one of which is modified by phosphorylation. The vast majority of E2F remains in the free form throughout the very early embryonic cell cycle, and it also remains predominantly free until some time after the mid-blastula transition, the onset of zygotic transcription. At this time, E2F complexes significantly to pRb but not to cdk2, although cdk2 binding is found in tissue culture cells from a very advanced stage in embryogenesis. This suggests that the complexing of E2F to cyclins, cyclin-dependent kinases, and tumor suppressor gene products may be controlled separately in early Xenopus development. Thus, the association of E2F with other molecules may not result solely from processes affecting cell cycle progression but may also reflect developmental and differentiation cues.
Baeuerle,
Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
1988, Pubmed
Baeuerle,
Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
1988,
Pubmed Bagchi,
The retinoblastoma protein copurifies with E2F-I, an E1A-regulated inhibitor of the transcription factor E2F.
1991,
Pubmed Bagchi,
Adenovirus E1A proteins can dissociate heteromeric complexes involving the E2F transcription factor: a novel mechanism for E1A trans-activation.
1990,
Pubmed Bandara,
Cyclin A and the retinoblastoma gene product complex with a common transcription factor.
1991,
Pubmed Bandara,
Functional synergy between DP-1 and E2F-1 in the cell cycle-regulating transcription factor DRTF1/E2F.
1993,
Pubmed Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Buchkovich,
The retinoblastoma protein is phosphorylated during specific phases of the cell cycle.
1989,
Pubmed Cao,
Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F.
1992,
Pubmed Chellappan,
The E2F transcription factor is a cellular target for the RB protein.
1991,
Pubmed Chittenden,
The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein.
1991,
Pubmed Cobrinik,
Cell cycle-specific association of E2F with the p130 E1A-binding protein.
1993,
Pubmed Cohen,
Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221.
1989,
Pubmed Cooper,
RB and the cell cycle: entrance or exit?
1989,
Pubmed Dalton,
Cell cycle regulation of the human cdc2 gene.
1992,
Pubmed DeCaprio,
The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory element.
1989,
Pubmed DePamphilis,
Transcriptional elements as components of eukaryotic origins of DNA replication.
1988,
Pubmed Destrée,
Structure and expression of the Xenopus retinoblastoma gene.
1992,
Pubmed
,
Xenbase Devoto,
A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex.
1992,
Pubmed Ewen,
Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins.
1992,
Pubmed Fang,
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.
1991,
Pubmed
,
Xenbase Girling,
A new component of the transcription factor DRTF1/E2F.
1993,
Pubmed Hamel,
Transcriptional repression of the E2-containing promoters EIIaE, c-myc, and RB1 by the product of the RB1 gene.
1992,
Pubmed Heintz,
Eukaryotic replication origins as promoters of bidirectional DNA synthesis.
1992,
Pubmed Helin,
The retinoblastoma protein as a transcriptional repressor.
1993,
Pubmed Helin,
Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.
1993,
Pubmed Helin,
A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.
1992,
Pubmed Hiebert,
The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F.
1992,
Pubmed Hu,
Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
1991,
Pubmed
,
Xenbase Huber,
Transcription factor E2F binds DNA as a heterodimer.
1993,
Pubmed Ivey-Hoyle,
Cloning and characterization of E2F-2, a novel protein with the biochemical properties of transcription factor E2F.
1993,
Pubmed Johnson,
Expression of transcription factor E2F1 induces quiescent cells to enter S phase.
1993,
Pubmed Kaelin,
Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties.
1992,
Pubmed Kaelin,
Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.
1991,
Pubmed Kimelman,
The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle.
1987,
Pubmed
,
Xenbase Kovesdi,
Identification of a cellular transcription factor involved in E1A trans-activation.
1986,
Pubmed La Thangue,
An adenovirus E1A-like transcription factor is regulated during the differentiation of murine embryonal carcinoma stem cells.
1987,
Pubmed La Thangue,
The embryonal carcinoma stem cell Ela-like activity involves a differentiation-regulated transcription factor.
1990,
Pubmed Lees,
The retinoblastoma protein binds to a family of E2F transcription factors.
1993,
Pubmed Lees,
Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.
1992,
Pubmed Mohun,
Temporal and tissue-specific expression of the proto-oncogene c-fos during development in Xenopus laevis.
1989,
Pubmed
,
Xenbase Mudryj,
Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A.
1991,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Nevins,
Transcriptional regulation. A closer look at E2F.
1992,
Pubmed Newport,
A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
1982,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Pagano,
Association of cdk2 kinase with the transcription factor E2F during S phase.
1992,
Pubmed Pudney,
Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.
1973,
Pubmed
,
Xenbase Ray,
Identification of a 60-kilodalton Rb-binding protein, RBP60, that allows the Rb-E2F complex to bind DNA.
1992,
Pubmed Reichel,
Developmental control of a promoter-specific factor that is also regulated by the E1A gene product.
1987,
Pubmed Schwarz,
Interactions of the p107 and Rb proteins with E2F during the cell proliferation response.
1993,
Pubmed Shan,
Molecular cloning of cellular genes encoding retinoblastoma-associated proteins: identification of a gene with properties of the transcription factor E2F.
1992,
Pubmed Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase Shirodkar,
The transcription factor E2F interacts with the retinoblastoma product and a p107-cyclin A complex in a cell cycle-regulated manner.
1992,
Pubmed Taylor,
Xenopus embryos contain a somite-specific, MyoD-like protein that binds to a promoter site required for muscle actin expression.
1991,
Pubmed
,
Xenbase Weintraub,
Retinoblastoma protein switches the E2F site from positive to negative element.
1992,
Pubmed Zamanian,
Adenovirus E1a prevents the retinoblastoma gene product from repressing the activity of a cellular transcription factor.
1992,
Pubmed
