Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1994 Jun 15;1312:2842-8. doi: 10.1002/j.1460-2075.1994.tb06578.x.
Show Gene links
Show Anatomy links
zeta PKC induces phosphorylation and inactivation of I kappa B-alpha in vitro.
Diaz-Meco MT, Dominguez I, Sanz L, Dent P, Lozano J, Municio MM, Berra E, Hay RT, Sturgill TW, Moscat J.
???displayArticle.abstract???
The zeta isotype of protein kinase C (zeta PKC), a distinct PKC unable to bind phorbol esters, is required during NF-kappa B activation as well as in mitogenic signalling in Xenopus oocytes and mammalian cells. To investigate the mechanism(s) for control of cellular functions by zeta PKC, this enzyme was expressed in Escherichia coli as a fusion protein with maltose binding protein (MBP), to allow immobilization on amylose beads to study signalling proteins in cell extracts that might form complex(es) with zeta PKC. The following evidence for interaction with the NF-kappa B/I kappa B pathway was obtained. MBP-zeta PKC, but not MBP, bound and activated a potentially novel I kappa B kinase of approximately 50 kDa molecular weight able to regulate I kappa B-alpha function. Activation of the I kappa B kinase was dependent on zeta PKC enzymatic activity and ATP, suggesting that zeta PKC controls, directly or indirectly, the activity of a functionally significant I kappa B kinase. Importantly, zeta PKC immunoprecipitates from TNF-alpha-stimulated NIH-3T3 fibroblasts displayed a higher I kappa B phosphorylating activity than untreated controls, indicating the in vivo relevance of these findings. We also show here that zeta PKC associates with and activates MKK-MAPK in vitro, suggesting that one of the mechanisms whereby overexpression of zeta PKC leads to deregulation of cell growth may be accounted for at least in part by activation of the MKK-MAPK complex. However, neither MKK nor MAPK is responsible for the putative I kappa B phosphorylating activity. These data provide a decisive step towards understanding the functions of zeta PKC.
Arenzana-Seisdedos,
Phosphatidylcholine hydrolysis activates NF-kappa B and increases human immunodeficiency virus replication in human monocytes and T lymphocytes.
1993, Pubmed
Arenzana-Seisdedos,
Phosphatidylcholine hydrolysis activates NF-kappa B and increases human immunodeficiency virus replication in human monocytes and T lymphocytes.
1993,
Pubmed Baeuerle,
I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
1988,
Pubmed Baeuerle,
Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
1988,
Pubmed Baldwin,
Induction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts.
1991,
Pubmed Beg,
Tumor necrosis factor and interleukin-1 lead to phosphorylation and loss of I kappa B alpha: a mechanism for NF-kappa B activation.
1993,
Pubmed Berra,
Protein kinase C zeta isoform is critical for mitogenic signal transduction.
1993,
Pubmed
,
Xenbase Brown,
Mutual regulation of the transcriptional activator NF-kappa B and its inhibitor, I kappa B-alpha.
1993,
Pubmed Bruder,
Oncogene activation of HIV-LTR-driven expression via the NF-kappa B binding sites.
1993,
Pubmed Cai,
Hydrolysis of phosphatidylcholine is stimulated by Ras proteins during mitogenic signal transduction.
1992,
Pubmed Cai,
Hydrolysis of phosphatidylcholine couples Ras to activation of Raf protein kinase during mitogenic signal transduction.
1993,
Pubmed Crews,
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
1992,
Pubmed Davis,
The mitogen-activated protein kinase signal transduction pathway.
1993,
Pubmed Dent,
Activation of mitogen-activated protein kinase kinase by v-Raf in NIH 3T3 cells and in vitro.
1992,
Pubmed Devary,
NF-kappa B activation by ultraviolet light not dependent on a nuclear signal.
1993,
Pubmed Diaz-Meco,
A dominant negative protein kinase C zeta subspecies blocks NF-kappa B activation.
1993,
Pubmed
,
Xenbase Dominguez,
Inhibition of protein kinase C zeta subspecies blocks the activation of an NF-kappa B-like activity in Xenopus laevis oocytes.
1993,
Pubmed
,
Xenbase Dominguez,
Evidence for a role of protein kinase C zeta subspecies in maturation of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase Finco,
Kappa B site-dependent induction of gene expression by diverse inducers of nuclear factor kappa B requires Raf-1.
1993,
Pubmed García de Herreros,
Requirement of phospholipase C-catalyzed hydrolysis of phosphatidylcholine for maturation of Xenopus laevis oocytes in response to insulin and ras p21.
1991,
Pubmed
,
Xenbase Ghosh,
Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B.
1990,
Pubmed Higgins,
Antisense inhibition of the p65 subunit of NF-kappa B blocks tumorigenicity and causes tumor regression.
1993,
Pubmed Howe,
Activation of the MAP kinase pathway by the protein kinase raf.
1992,
Pubmed Kameshita,
A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel.
1989,
Pubmed Kitajima,
Ablation of transplanted HTLV-I Tax-transformed tumors in mice by antisense inhibition of NF-kappa B.
1992,
Pubmed Kyriakis,
Mitogen regulation of c-Raf-1 protein kinase activity toward mitogen-activated protein kinase-kinase.
1993,
Pubmed Kyriakis,
Raf-1 activates MAP kinase-kinase.
1992,
Pubmed Lange-Carter,
A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.
1993,
Pubmed Larrodera,
Phospholipase C-mediated hydrolysis of phosphatidylcholine is an important step in PDGF-stimulated DNA synthesis.
1990,
Pubmed Lenardo,
NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.
1989,
Pubmed Li,
Raf-1 protein kinase activates the NF-kappa B transcription factor by dissociating the cytoplasmic NF-kappa B-I kappa B complex.
1993,
Pubmed Meichle,
Protein kinase C-independent activation of nuclear factor kappa B by tumor necrosis factor.
1990,
Pubmed Muslin,
Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
1993,
Pubmed
,
Xenbase Nishizuka,
Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C.
1992,
Pubmed Paya,
NF-kappa B-dependent induction of the NF-kappa B p50 subunit gene promoter underlies self-perpetuation of human immunodeficiency virus transcription in monocytic cells.
1992,
Pubmed Pears,
The regulatory domain of protein kinase C-epsilon restricts the catalytic-domain-specificity.
1991,
Pubmed Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Schütze,
TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced "acidic" sphingomyelin breakdown.
1992,
Pubmed Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase Shirakawa,
In vitro activation and nuclear translocation of NF-kappa B catalyzed by cyclic AMP-dependent protein kinase and protein kinase C.
1989,
Pubmed Williams,
Both p21ras and pp60v-src are required, but neither alone is sufficient, to activate the Raf-1 kinase.
1992,
Pubmed Wu,
Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.
1993,
Pubmed
