Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Mitogen-activated protein kinases (MAP kinases) are active only when phosphorylated. Here we examine whether the activation of Xenopus p42MAP kinase might involve changes in its association with other proteins as well as changes in its phosphorylation state. We find that when p42MAP kinase is phosphorylated and active, it is monomeric, and that when p42MAP kinase is nonphosphorylated and inactive, about half of it is monomeric and half is a component of a 110-kDa complex. We identify Rsk, an 82-kDa protein kinase that can be phosphorylated and partially activated by p42MAP kinase, as being specifically associated with inactive p42MAP kinase. It is possible that the complex of inactive p42MAP kinase and inactive Rsk acts as a single signal reception particle and that the activation of the two kinases may be better described as a fork in a bifurcating signal transduction pathway than as successive levels in a kinase cascade.
Barrett,
A purified S6 kinase kinase from Xenopus eggs activates S6 kinase II and autophosphorylates on serine, threonine, and tyrosine residues.
1992, Pubmed,
Xenbase
Barrett,
A purified S6 kinase kinase from Xenopus eggs activates S6 kinase II and autophosphorylates on serine, threonine, and tyrosine residues.
1992,
Pubmed
,
Xenbase Boulton,
Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.
1991,
Pubmed Chen,
Nuclear localization and regulation of erk- and rsk-encoded protein kinases.
1992,
Pubmed Chou,
Inhibition of c-Jun DNA binding by mitogen-activated protein kinase.
1992,
Pubmed
,
Xenbase Crews,
Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine.
1991,
Pubmed Crews,
Extracellular signals and reversible protein phosphorylation: what to Mek of it all.
1993,
Pubmed Davis,
The mitogen-activated protein kinase signal transduction pathway.
1993,
Pubmed Erikson,
Xenopus ribosomal protein S6 kinase II.
1991,
Pubmed
,
Xenbase Errede,
A conserved kinase cascade for MAP kinase activation in yeast.
1993,
Pubmed Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase Haccard,
In vivo activation of a microtubule-associated protein kinase during meiotic maturation of the Xenopus oocyte.
1990,
Pubmed
,
Xenbase Itoh,
The post-translational processing of ras p21 is critical for its stimulation of mitogen-activated protein kinase.
1993,
Pubmed
,
Xenbase Kosako,
cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.
1993,
Pubmed
,
Xenbase Kosako,
Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation.
1992,
Pubmed
,
Xenbase Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed
,
Xenbase Moodie,
Complexes of Ras.GTP with Raf-1 and mitogen-activated protein kinase kinase.
1993,
Pubmed Nebreda,
The c-mos proto-oncogene protein kinase turns on and maintains the activity of MAP kinase, but not MPF, in cell-free extracts of Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase Pawson,
SH2 and SH3 domains: from structure to function.
1992,
Pubmed Payne,
Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).
1991,
Pubmed Posada,
Mos stimulates MAP kinase in Xenopus oocytes and activates a MAP kinase kinase in vitro.
1993,
Pubmed
,
Xenbase Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase Ray,
Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
1988,
Pubmed Sanghera,
Immunological characterization of avian MAP kinases: evidence for nuclear localization.
1992,
Pubmed Scimeca,
Nerve growth factor-induced phosphorylation cascade in PC12 pheochromocytoma cells. Association of S6 kinase II with the microtubule-associated protein kinase, ERK1.
1992,
Pubmed Seger,
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.
1991,
Pubmed Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase Shibuya,
Mos induces the in vitro activation of mitogen-activated protein kinases in lysates of frog oocytes and mammalian somatic cells.
1993,
Pubmed
,
Xenbase Smith,
Oogenesis and oocyte isolation.
1991,
Pubmed
,
Xenbase Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase Wu,
Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.
1991,
Pubmed
