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T-type and N-type calcium channels of Xenopus oocytes: evidence for specific interactions with beta subunits.
Lacerda AE, Perez-Reyes E, Wei X, Castellano A, Brown AM.
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We used amplifying effects of calcium channel beta subunits to identify endogenous calcium channels in Xenopus oocytes. Expression of rat brain beta 4 increased macroscopic endogenous current magnitude with a small effect on kinetics. In contrast, expression of rat brain/cardiac beta 2 produced a much larger increase in current magnitude and dramatically slowed current decay. Low concentrations of omega-conotoxin GVIA irreversibly blocked currents in both uninjected and beta 2-injected oocytes. Single channel recordings revealed both T- and N-type calcium channels with conductances of 9 and 18 pS, respectively, in uninjected oocytes and in oocytes expressing either beta subunit. Expression of either beta subunit slowed average current decay of T-type single channels. Slowing of T-type current decay by expression of beta 2 was due to reopening of the channels. N-type single channel average current decay showed little change with expression of beta 4, whereas expression of beta 2 slowed average current decay.
Aldrich,
A reinterpretation of mammalian sodium channel gating based on single channel recording.
, Pubmed
Aldrich,
A reinterpretation of mammalian sodium channel gating based on single channel recording.
,
Pubmed Artalejo,
Omega-conotoxin GVIA blocks a Ca2+ current in bovine chromaffin cells that is not of the "classic" N type.
1992,
Pubmed Bean,
Classes of calcium channels in vertebrate cells.
1989,
Pubmed Biel,
Primary structure and functional expression of a high voltage activated calcium channel from rabbit lung.
1990,
Pubmed
,
Xenbase Bourinet,
Endogenous Xenopus-oocyte Ca-channels are regulated by protein kinases A and C.
1992,
Pubmed
,
Xenbase Brust,
Human neuronal voltage-dependent calcium channels: studies on subunit structure and role in channel assembly.
1993,
Pubmed Carbone,
Neuronal calcium channels: kinetics, blockade and modulation.
1989,
Pubmed Carbone,
A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones.
,
Pubmed Carbone,
Single low-voltage-activated calcium channels in chick and rat sensory neurones.
1987,
Pubmed Castellano,
Cloning and expression of a neuronal calcium channel beta subunit.
1993,
Pubmed
,
Xenbase Castellano,
Cloning and expression of a third calcium channel beta subunit.
1993,
Pubmed
,
Xenbase Catterall,
Functional subunit structure of voltage-gated calcium channels.
1991,
Pubmed Chen,
Mechanism of gating of T-type calcium channels.
1990,
Pubmed Colquhoun,
Fast events in single-channel currents activated by acetylcholine and its analogues at the frog muscle end-plate.
1985,
Pubmed Dascal,
Calcium channel currents in Xenopus oocytes injected with rat skeletal muscle RNA.
1992,
Pubmed
,
Xenbase Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase Droogmans,
Kinetic properties of the cardiac T-type calcium channel in the guinea-pig.
1989,
Pubmed Durroux,
Three components of the calcium current in cultured glomerulosa cells from rat adrenal gland.
1988,
Pubmed Ellinor,
Functional expression of a rapidly inactivating neuronal calcium channel.
1993,
Pubmed
,
Xenbase Feldman,
Omega Conus geographus toxin: a peptide that blocks calcium channels.
1987,
Pubmed Fox,
Single-channel recordings of three types of calcium channels in chick sensory neurones.
1987,
Pubmed Hess,
Calcium channels in vertebrate cells.
1990,
Pubmed Itagaki,
Native-type DHP-sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac alpha 1 subunits expressed in Xenopus laevis oocytes and are regulated by alpha 2- and beta-subunits.
1992,
Pubmed
,
Xenbase Kunze,
Cardiac Na currents and the inactivating, reopening, and waiting properties of single cardiac Na channels.
1985,
Pubmed Matteson,
Properties of two types of calcium channels in clonal pituitary cells.
1986,
Pubmed McCleskey,
Omega-conotoxin: direct and persistent blockade of specific types of calcium channels in neurons but not muscle.
1987,
Pubmed Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase Mikami,
Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.
1989,
Pubmed
,
Xenbase Miller,
Voltage-sensitive Ca2+ channels.
1992,
Pubmed Mintz,
Inhibition of N- and L-type Ca2+ channels by the spider venom toxin omega-Aga-IIIA.
1991,
Pubmed Mintz,
P-type calcium channels in rat central and peripheral neurons.
1992,
Pubmed Mori,
Primary structure and functional expression from complementary DNA of a brain calcium channel.
1991,
Pubmed
,
Xenbase Nargeot,
Heterologous expression of calcium channels.
1992,
Pubmed Nilius,
A novel type of cardiac calcium channel in ventricular cells.
,
Pubmed Nowycky,
Three types of neuronal calcium channel with different calcium agonist sensitivity.
,
Pubmed Perez-Reyes,
Cloning and expression of a cardiac/brain beta subunit of the L-type calcium channel.
1992,
Pubmed
,
Xenbase Plummer,
Elementary properties and pharmacological sensitivities of calcium channels in mammalian peripheral neurons.
1989,
Pubmed Plummer,
Reversible uncoupling of inactivation in N-type calcium channels.
1991,
Pubmed Sher,
Omega-conotoxin-sensitive voltage-operated calcium channels in vertebrate cells.
1991,
Pubmed Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase Singer,
The roles of the subunits in the function of the calcium channel.
1991,
Pubmed
,
Xenbase Singer-Lahat,
Evidence for the existence of RNA of Ca(2+)-channel alpha 2/delta subunit in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Singer-Lahat,
Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase C.
1992,
Pubmed
,
Xenbase Soong,
Structure and functional expression of a member of the low voltage-activated calcium channel family.
1993,
Pubmed Stea,
A beta-subunit normalizes the electrophysiological properties of a cloned N-type Ca2+ channel alpha 1-subunit.
1993,
Pubmed
,
Xenbase Swandulla,
Do calcium channel classifications account for neuronal calcium channel diversity?
1991,
Pubmed Tsien,
Calcium channels, stores, and oscillations.
1990,
Pubmed Umbach,
Expression of an omega-conotoxin-sensitive calcium channel in Xenopus oocytes injected with mRNA from Torpedo electric lobe.
1987,
Pubmed
,
Xenbase Wei,
Heterologous regulation of the cardiac Ca2+ channel alpha 1 subunit by skeletal muscle beta and gamma subunits. Implications for the structure of cardiac L-type Ca2+ channels.
1991,
Pubmed
,
Xenbase Williams,
Structure and functional expression of alpha 1, alpha 2, and beta subunits of a novel human neuronal calcium channel subtype.
1992,
Pubmed
,
Xenbase
