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Wnt-4 is expressed in developing neural and renal tissue and is required for renal tubulogenesis in mouse and Xenopus. The function of Wnt-4 in neural differentiation is unknown so far. Here we demonstrate that Wnt-4 is required for eye development in Xenopus laevis. This effect of Wnt-4 depends on the activation of a beta-catenin-independent, noncanonical Wnt signaling pathway. Furthermore, we report the identification of EAF2, a component of the ELL-mediated RNA polymerase II elongation factor complex, as a target gene of Wnt-4 signaling. EAF2 is specifically expressed in the eye and EAF2 expression was dependent on Wnt-4 function. Loss of EAF2 function results in loss of eyes and loss of Wnt-4 function could be rescued by EAF2. In neuralized animal caps, EAF2 has properties characteristic for an RNA polymerase II elongation factor regulating the expression of the eye-specific transcription factor Rx. These data add a new layer of complexity to our understanding of eye development and give further evidence for the importance of noncanonical Wnt pathways in organ development.
Bennett,
SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.
2001, Pubmed
Bennett,
SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase.
2001,
Pubmed Boutros,
Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling.
1998,
Pubmed Cai,
Phosphorylation of Pax2 by the c-Jun N-terminal kinase and enhanced Pax2-dependent transcription activation.
2002,
Pubmed Chen,
Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins.
2005,
Pubmed Chow,
Pax6 induces ectopic eyes in a vertebrate.
1999,
Pubmed
,
Xenbase Chow,
Early eye development in vertebrates.
2001,
Pubmed Dabdoub,
Wnt signaling mediates reorientation of outer hair cell stereociliary bundles in the mammalian cochlea.
2003,
Pubmed Darken,
The planar polarity gene strabismus regulates convergent extension movements in Xenopus.
2002,
Pubmed
,
Xenbase Du,
Identification of distinct classes and functional domains of Wnts through expression of wild-type and chimeric proteins in Xenopus embryos.
1995,
Pubmed
,
Xenbase Eissenberg,
dELL is an essential RNA polymerase II elongation factor with a general role in development.
2002,
Pubmed Guo,
A regulator of transcriptional elongation controls vertebrate neuronal development.
2000,
Pubmed Hollyday,
Wnt expression patterns in chick embryo nervous system.
1995,
Pubmed Keegan,
The elongation factors Pandora/Spt6 and Foggy/Spt5 promote transcription in the zebrafish embryo.
2002,
Pubmed Kiecker,
A morphogen gradient of Wnt/beta-catenin signalling regulates anteroposterior neural patterning in Xenopus.
2001,
Pubmed
,
Xenbase Kühl,
Ca(2+)/calmodulin-dependent protein kinase II is stimulated by Wnt and Frizzled homologs and promotes ventral cell fates in Xenopus.
2000,
Pubmed
,
Xenbase Kühl,
The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape.
2000,
Pubmed Li,
Expression of murine ELL-associated factor 2 (Eaf2) is developmentally regulated.
2003,
Pubmed Liu,
G protein signaling from activated rat frizzled-1 to the beta-catenin-Lef-Tcf pathway.
2001,
Pubmed
,
Xenbase Liu,
Activation of a frizzled-2/beta-adrenergic receptor chimera promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via Galphao and Galphat.
1999,
Pubmed Logan,
The Wnt signaling pathway in development and disease.
2004,
Pubmed Lyuksyutova,
Anterior-posterior guidance of commissural axons by Wnt-frizzled signaling.
2003,
Pubmed Mathers,
The Rx homeobox gene is essential for vertebrate eye development.
1997,
Pubmed
,
Xenbase McGrew,
Analysis of Xwnt-4 in embryos of Xenopus laevis: a Wnt family member expressed in the brain and floor plate.
1992,
Pubmed
,
Xenbase Mlodzik,
Planar polarity in the Drosophila eye: a multifaceted view of signaling specificity and cross-talk.
1999,
Pubmed Naylor,
Retroviral expression of Wnt-1 and Wnt-7b produces different effects in mouse mammary epithelium.
2000,
Pubmed Neumann,
Long-range action of Wingless organizes the dorsal-ventral axis of the Drosophila wing.
1997,
Pubmed Parr,
Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds.
1993,
Pubmed Rasmussen,
Regulation of eye development by frizzled signaling in Xenopus.
2001,
Pubmed
,
Xenbase Rosso,
Wnt signaling through Dishevelled, Rac and JNK regulates dendritic development.
2005,
Pubmed Royet,
Establishing primordia in the Drosophila eye-antennal imaginal disc: the roles of decapentaplegic, wingless and hedgehog.
1997,
Pubmed Saulnier,
Essential function of Wnt-4 for tubulogenesis in the Xenopus pronephric kidney.
2002,
Pubmed
,
Xenbase Shilatifard,
The RNA polymerase II elongation complex.
2003,
Pubmed Simone,
ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties.
2003,
Pubmed Stark,
Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4.
1994,
Pubmed Stump,
A role for Wnt/beta-catenin signaling in lens epithelial differentiation.
2003,
Pubmed Veeman,
A second canon. Functions and mechanisms of beta-catenin-independent Wnt signaling.
2003,
Pubmed Weston,
JNK initiates a cytokine cascade that causes Pax2 expression and closure of the optic fissure.
2003,
Pubmed Wolda,
Cloning and developmental expression in Xenopus laevis of seven additional members of the Wnt family.
1992,
Pubmed
,
Xenbase Zecca,
Direct and long-range action of a wingless morphogen gradient.
1996,
Pubmed Zhang,
Function of Rx, but not Pax6, is essential for the formation of retinal progenitor cells in mice.
2000,
Pubmed Zuber,
Specification of the vertebrate eye by a network of eye field transcription factors.
2003,
Pubmed
,
Xenbase
