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Mini spindles, the XMAP215 homologue, suppresses pausing of interphase microtubules in Drosophila.
Brittle AL, Ohkura H.
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Drosophila Mini spindles (Msps) protein belongs to a conserved family of microtubule-associated proteins (MAPs). Intriguingly, this family of MAPs, including Xenopus XMAP215, was reported to have both microtubule stabilising and destabilising activities. While they are shown to regulate various aspects of microtubules, the role in regulating interphase microtubules in animal cells has yet to be established. Here, we show that the depletion or mutation of Msps prevents interphase microtubules from extending to the cell periphery and leads to the formation of stable microtubule bundles. The effect is independent of known Msps regulator or effector proteins, kinesin-13/KinI homologues or D-TACC. Real-time analysis revealed that the depletion of Msps results in a dramatic increase of microtubule pausing with little or no growth. Our study provides the first direct evidence to support a hypothesis that this family of MAPs acts as an antipausing factor to exhibit both microtubule stabilising and destabilising activities.
Arnal,
Structural transitions at microtubule ends correlate with their dynamic properties in Xenopus egg extracts.
2000, Pubmed,
Xenbase
Arnal,
Structural transitions at microtubule ends correlate with their dynamic properties in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Bellanger,
TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos.
2003,
Pubmed Bu,
Regulation of microtubule assembly by human EB1 family proteins.
2001,
Pubmed Cassimeris,
Real-time observations of microtubule dynamic instability in living cells.
1988,
Pubmed Charrasse,
The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.
1998,
Pubmed
,
Xenbase Clemens,
Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways.
2000,
Pubmed Cullen,
Msps protein is localized to acentrosomal poles to ensure bipolarity of Drosophila meiotic spindles.
2001,
Pubmed Cullen,
mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.
1999,
Pubmed
,
Xenbase Garcia,
Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint.
2001,
Pubmed
,
Xenbase Gard,
A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end.
1987,
Pubmed
,
Xenbase Gergely,
D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo.
2000,
Pubmed Gergely,
The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells.
2003,
Pubmed Goshima,
The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line.
2003,
Pubmed Gräf,
Regulated expression of the centrosomal protein DdCP224 affects microtubule dynamics and reveals mechanisms for the control of supernumerary centrosome number.
2003,
Pubmed Gräf,
Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication.
2000,
Pubmed Haren,
Direct binding of NuMA to tubulin is mediated by a novel sequence motif in the tail domain that bundles and stabilizes microtubules.
2002,
Pubmed
,
Xenbase Holmfeldt,
Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis.
2004,
Pubmed Kinoshita,
XMAP215: a key component of the dynamic microtubule cytoskeleton.
2002,
Pubmed
,
Xenbase Kinoshita,
Reconstitution of physiological microtubule dynamics using purified components.
2001,
Pubmed
,
Xenbase Komarova,
Life cycle of MTs: persistent growth in the cell interior, asymmetric transition frequencies and effects of the cell boundary.
2002,
Pubmed Kosco,
Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast.
2001,
Pubmed
,
Xenbase Lee,
Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour.
2001,
Pubmed
,
Xenbase Matthews,
ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.
1998,
Pubmed Mitchison,
Dynamic instability of microtubule growth.
,
Pubmed Moon,
The Drosophila microtubule-associated protein mini spindles is required for cytoplasmic microtubules in oogenesis.
2004,
Pubmed Nakaseko,
M phase-specific kinetochore proteins in fission yeast: microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase.
2001,
Pubmed Ohkura,
Dis1/TOG universal microtubule adaptors - one MAP for all?
2001,
Pubmed Popov,
XMAP215 regulates microtubule dynamics through two distinct domains.
2001,
Pubmed
,
Xenbase Radcliffe,
Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.
1998,
Pubmed Raemaekers,
NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization.
2003,
Pubmed Rogers,
Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase.
2004,
Pubmed Rogers,
Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle.
2002,
Pubmed Rusan,
Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin.
2001,
Pubmed Sato,
Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation.
2004,
Pubmed Severin,
Stu2 promotes mitotic spindle elongation in anaphase.
2001,
Pubmed Shelden,
Observation and quantification of individual microtubule behavior in vivo: microtubule dynamics are cell-type specific.
1993,
Pubmed Shirasu-Hiza,
Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification.
2003,
Pubmed
,
Xenbase Smith,
Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.
1984,
Pubmed Srayko,
Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules.
2003,
Pubmed Tirnauer,
Yeast Bim1p promotes the G1-specific dynamics of microtubules.
1999,
Pubmed Tirouvanziam,
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
2004,
Pubmed Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Tran,
A metastable intermediate state of microtubule dynamic instability that differs significantly between plus and minus ends.
1997,
Pubmed Usui,
The XMAP215 homologue Stu2 at yeast spindle pole bodies regulates microtubule dynamics and anchorage.
2003,
Pubmed van Breugel,
Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer.
2003,
Pubmed
,
Xenbase Vasquez,
XMAP from Xenopus eggs promotes rapid plus end assembly of microtubules and rapid microtubule polymer turnover.
1994,
Pubmed
,
Xenbase Wang,
Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.
1997,
Pubmed Whittington,
MOR1 is essential for organizing cortical microtubules in plants.
2001,
Pubmed
