Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-21573
J Mol Biol 1994 Feb 25;2363:685-90. doi: 10.1006/jmbi.1994.1180.
Show Gene links Show Anatomy links

Nucleosome structural changes due to acetylation.

Bauer WR, Hayes JJ, White JH, Wolffe AP.


???displayArticle.abstract???
We have measured the helical repeat of acetylated nucleosomes reconstituted with HeLa cell histone octamers and the 5 S RNA gene positioning sequence from Xenopus borealis. The hydroxyl radical footprinting experiments show that the helical repeat of nucleosome-bound DNA is unchanged upon acetylation, and that the extent of DNA-protein contacts remains unaltered. These results, combined with previous determinations of the linking number change in minichromosomes as a function of the extent of acetylation, are analyzed with surface linking theory. We demonstrate that the shape of the nucleosome changes upon acetylation, in such a way as to decrease the number of times the DNA winds around the nucleosome central axis.

???displayArticle.pubmedLink??? 8114086
???displayArticle.link??? J Mol Biol