Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Internal Na+ and Mg2+ blockade of DRK1 (Kv2.1) potassium channels expressed in Xenopus oocytes. Inward rectification of a delayed rectifier.
Lopatin AN, Nichols CG.
???displayArticle.abstract???
Delayed rectifier potassium channels were expressed in the membrane of Xenopus oocytes by injection of rat brainDRK1 (Kv2.1) cRNA, and currents were measured in cell-attached and inside-out patch configurations. In intact cells the current-voltage relationship displayed inward going rectification at potentials > +100 mV. Rectification was abolished by excision of membrane patches into solutions containing no Mg2+ or Na+ ions, but was restored by introducing Mg2+ or Na+ ions into the bath solution. At +50 mV, half-maximum blocking concentrations for Mg2+ and Na+ were 4.8 +/- 2.5 mM (n = 6) and 26 +/- 4 mM (n = 3) respectively. Increasing extracellular potassium concentration reduced the degree of rectification of intact cells. It is concluded that inward going rectification resulting from voltage-dependent block by internal cations can be observed with normally outwardly rectifying DRK1 channels.
Bezanilla,
Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons.
1972, Pubmed
Bezanilla,
Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons.
1972,
Pubmed Covarrubias,
Shaker, Shal, Shab, and Shaw express independent K+ current systems.
1991,
Pubmed
,
Xenbase de Laat,
Intracellular ionic distribution, cell membrane permeability and membrane potential of the Xenopus egg during first cleavage.
1974,
Pubmed
,
Xenbase Findlay,
The effects of magnesium upon adenosine triphosphate-sensitive potassium channels in a rat insulin-secreting cell line.
1987,
Pubmed Forsythe,
Unitary A-currents of rat locus coeruleus neurones grown in cell culture: rectification caused by internal Mg2+ and Na+.
1992,
Pubmed Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase French,
Sodium ions as blocking agents and charge carriers in the potassium channel of the squid giant axon.
1977,
Pubmed Heginbotham,
A functional connection between the pores of distantly related ion channels as revealed by mutant K+ channels.
1992,
Pubmed
,
Xenbase Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed Hille,
Potassium channels as multi-ion single-file pores.
1978,
Pubmed Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase Horie,
Voltage-dependent magnesium block of adenosine-triphosphate-sensitive potassium channel in guinea-pig ventricular cells.
1987,
Pubmed Kasai,
Single transient K channels in mammalian sensory neurons.
1986,
Pubmed Kelly,
Inwardly rectifying potassium current in rabbit osteoclasts: a whole-cell and single-channel study.
1992,
Pubmed Kirsch,
Differences between the deep pores of K+ channels determined by an interacting pair of nonpolar amino acids.
1992,
Pubmed
,
Xenbase Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase Latorre,
K+ channels gated by voltage and ions.
1984,
Pubmed Lu,
A delayed rectifier potassium current in Xenopus oocytes.
1990,
Pubmed
,
Xenbase MacKinnon,
Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels.
1990,
Pubmed Marty,
Blocking of large unitary calcium-dependent potassium currents by internal sodium ions.
1983,
Pubmed Matsuda,
Single inwardly rectifying potassium channels in cultured muscle cells from rat and mouse.
1989,
Pubmed Matsuda,
Ohmic conductance through the inwardly rectifying K channel and blocking by internal Mg2+.
,
Pubmed Matsuda,
Open-state substructure of inwardly rectifying potassium channels revealed by magnesium block in guinea-pig heart cells.
1988,
Pubmed Matsuda,
Triple-barrel structure of inwardly rectifying K+ channels revealed by Cs+ and Rb+ block in guinea-pig heart cells.
1989,
Pubmed Matsuda,
Effects of external and internal K+ ions on magnesium block of inwardly rectifying K+ channels in guinea-pig heart cells.
1991,
Pubmed Miledi,
Chloride current induced by injection of calcium into Xenopus oocytes.
1984,
Pubmed
,
Xenbase Oliva,
The mechanism of rectification of iK1 in canine Purkinje myocytes.
1990,
Pubmed Rettig,
Characterization of a Shaw-related potassium channel family in rat brain.
1992,
Pubmed
,
Xenbase Silver,
Intrinsic gating of inward rectifier in bovine pulmonary artery endothelial cells in the presence or absence of internal Mg2+.
1990,
Pubmed Swanson,
Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain.
1990,
Pubmed
,
Xenbase Vandenberg,
Inward rectification of a potassium channel in cardiac ventricular cells depends on internal magnesium ions.
1987,
Pubmed Woodhull,
Ionic blockage of sodium channels in nerve.
1973,
Pubmed Yellen,
Relief of Na+ block of Ca2+-activated K+ channels by external cations.
1984,
Pubmed Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed
