Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pediatr Nephrol
1993 Dec 01;76:823-6. doi: 10.1007/bf01213368.
Show Gene links
Show Anatomy links
Effect of low-phosphate diet on sodium/phosphate cotransport mRNA and protein content and on oocyte expression of phosphate transport.
Biber J, Caderas G, Stange G, Werner A, Murer H.
???displayArticle.abstract???
Recently, we have isolated a complementary DNA most likely related to rabbit kidney cortex brush border membrane sodium/phosphate (Na/Pi) cotransport activity [NaPi-1 (1)]. To further elucidate the cellular mechanisms involved in dietary 'adaptation' of renal Na/Pi cotransport, we have exposed young rabbits for 2 weeks to either a low phosphate (Pi) diet (LPD) or a high Pi diet (HPD). Initial linear uptake of Na/Pi cotransport in isolated brush border membrane vesicles was increased in rabbits on a LPD compared with those on a HPD. Injection of equal amounts of total mRNA isolated from kidney cortex of LPD or HPD rabbits into Xenopus laevis oocytes resulted in a higher stimulation of Na-dependent oocyte Pi uptake in LPD than HPD preparations. No difference in the content of 'specific' mRNA (NaPi-1 cDNA probe, Northern blots) and of the content of the 'specific' brush border membrane protein (NaPi-1 antipeptide antibody, Western blots) between LPD and HPD preparations was observed. We conclude that 'chronic' dietary Pi deprivation leads to a protein synthesis-dependent alteration of Na/Pi cotransport activity which does not involve a change in the total amount of a protein related to the recently cloned NaPi-1 protein.
Bertran,
Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.
1992, Pubmed,
Xenbase
Bertran,
Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.
1992,
Pubmed
,
Xenbase Biber,
A high yield preparation for rat kidney brush border membranes. Different behaviour of lysosomal markers.
1981,
Pubmed Biber,
Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry.
1993,
Pubmed Biber,
Na-Pi cotransport in LLC-PK1 cells: fast adaptive response to Pi deprivation.
1985,
Pubmed Biber,
Modulation of Na+-Pi cotransport in opossum kidney cells by extracellular phosphate.
1988,
Pubmed Brunette,
Phosphate uptake by superficial and deep nephron brush border membranes. Effect of the dietary phosphate and parathyroid hormone.
1984,
Pubmed Coady,
Sequence homologies among intestinal and renal Na+/glucose cotransporters.
1990,
Pubmed Escoubet,
Adaptation to Pi deprivation of cell Na-dependent Pi uptake: a widespread process.
1989,
Pubmed Hagenbuch,
Phosphate transport across the basolateral membrane from rat kidney cortex: sodium-dependence?
1986,
Pubmed Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase Levi,
Cholesterol modulates rat renal brush border membrane phosphate transport.
1990,
Pubmed Magagnin,
Expression cloning of human and rat renal cortex Na/Pi cotransport.
1993,
Pubmed
,
Xenbase Murer,
Homer Smith Award. Cellular mechanisms in proximal tubular Pi reabsorption: some answers and more questions.
1992,
Pubmed Murer,
Cellular mechanisms in proximal tubular reabsorption of inorganic phosphate.
1991,
Pubmed
,
Xenbase Pajor,
Molecular evidence for two renal Na+/glucose cotransporters.
1992,
Pubmed
,
Xenbase Shah,
Renal adaptation to a low phosphate diet in rats.
1979,
Pubmed Szczepanska-Konkel,
Phosphonocarboxylic acids as specific inhibitors of Na+-dependent transport of phosphate across renal brush border membrane.
1986,
Pubmed Turner,
Phosphate transport by brushborder membranes from superficial and juxtamedullary cortex.
1985,
Pubmed Werner,
Expression of renal transport systems for inorganic phosphate and sulfate in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase Yusufi,
Different mechanisms of adaptive increase in Na+-Pi cotransport across renal brush-border membrane.
1989,
Pubmed
