Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1993 Nov 01;9021:10235-9.
Show Gene links
Show Anatomy links
Atrial G protein-activated K+ channel: expression cloning and molecular properties.
Dascal N, Schreibmayer W, Lim NF, Wang W, Chavkin C, DiMagno L, Labarca C, Kieffer BL, Gaveriaux-Ruff C, Trollinger D.
???displayArticle.abstract???
Activity of several ion channels is controlled by heterotrimeric GTP-binding proteins (G proteins) via a membrane-delimited pathway that does not involve cytoplasmic intermediates. The best studied example is the K+ channel activated by muscarinic agonists in the atrium, which plays a crucial role in regulating the heartbeat. To enable studies of the molecular mechanisms of activation, this channel, denoted KGA, was cloned from a rat atrium cDNA library by functional coupling to coexpressed serotonin type 1A receptors in Xenopus oocytes. KGA displays regions of sequence homology to other inwardly rectifying channels as well as unique regions that may govern G-protein interaction. The expressed KGA channel is activated by serotonin 1A, muscarinic m2, and delta-opioid receptors via G proteins. KGA is activated by guanosine 5'-[gamma-thio]triphosphate in excised patches, confirming activation by a membrane-delimited pathway, and displays a conductance equal to that of the endogenous channel in atrial cells. The hypothesis that similar channels play a role in neuronal inhibition is supported by the cloning of a nearly identical channel (KGB1) from a rat brain cDNA library.
Andrade,
Pharmacologically distinct actions of serotonin on single pyramidal neurones of the rat hippocampus recorded in vitro.
1987, Pubmed
Andrade,
Pharmacologically distinct actions of serotonin on single pyramidal neurones of the rat hippocampus recorded in vitro.
1987,
Pubmed Andrade,
A G protein couples serotonin and GABAB receptors to the same channels in hippocampus.
1986,
Pubmed Bechem,
Removal of Ca current inactivation in dialysed guinea-pig atrial cardioballs by Ca chelators.
1985,
Pubmed Breitwieser,
Uncoupling of cardiac muscarinic and beta-adrenergic receptors from ion channels by a guanine nucleotide analogue.
,
Pubmed Brown,
Ionic channels and their regulation by G protein subunits.
1990,
Pubmed Catterall,
Structure and function of voltage-sensitive ion channels.
1988,
Pubmed Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase Dascal,
Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.
1993,
Pubmed
,
Xenbase EDWARDS,
Changes in membrane characteristics of heart muscle during inhibition.
1956,
Pubmed Guy,
Pursuing the structure and function of voltage-gated channels.
1990,
Pubmed
,
Xenbase Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Hammer,
Pirenzepine distinguishes between different subclasses of muscarinic receptors.
1980,
Pubmed Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase Horie,
Rectification of muscarinic K+ current by magnesium ion in guinea pig atrial cells.
1987,
Pubmed Hoyer,
5-HT receptors: subtypes and second messengers.
1991,
Pubmed Ito,
On the mechanism of G protein beta gamma subunit activation of the muscarinic K+ channel in guinea pig atrial cell membrane. Comparison with the ATP-sensitive K+ channel.
1992,
Pubmed Kaibara,
Regulation of spontaneous opening of muscarinic K+ channels in rabbit atrium.
1991,
Pubmed Karschin,
Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.
1991,
Pubmed Kennelly,
Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
1991,
Pubmed Kieffer,
The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.
1992,
Pubmed Kim,
Modulation of acetylcholine-activated K+ channel function in rat atrial cells by phosphorylation.
1991,
Pubmed Kirsch,
Alpha-subunit of Gk activates atrial K+ channels of chick, rat, and guinea pig.
1988,
Pubmed Kobilka,
An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
,
Pubmed Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase Kurachi,
G protein activation of cardiac muscarinic K+ channels.
1992,
Pubmed Kurachi,
Positive cooperativity in activation of the cardiac muscarinic K+ channel by intracellular GTP.
1990,
Pubmed Kurachi,
Short-term desensitization of muscarinic K+ channel current in isolated atrial myocytes and possible role of GTP-binding proteins.
1987,
Pubmed Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed Logothetis,
The beta gamma subunits of GTP-binding proteins activate the muscarinic K+ channel in heart.
,
Pubmed Mager,
Steady states, charge movements, and rates for a cloned GABA transporter expressed in Xenopus oocytes.
1993,
Pubmed
,
Xenbase Mohana Rao,
A conformational preference parameter to predict helices in integral membrane proteins.
1986,
Pubmed North,
Twelfth Gaddum memorial lecture. Drug receptors and the inhibition of nerve cells.
1989,
Pubmed Okabe,
The nature and origin of spontaneous noise in G protein-gated ion channels.
1991,
Pubmed Peralta,
Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.
1987,
Pubmed Pfaffinger,
GTP-binding proteins couple cardiac muscarinic receptors to a K channel.
,
Pubmed Portoghese,
Naltrindole, a highly selective and potent non-peptide delta opioid receptor antagonist.
1988,
Pubmed Ruoslahti,
Integrins.
1991,
Pubmed Sakmann,
Acetylcholine activation of single muscarinic K+ channels in isolated pacemaker cells of the mammalian heart.
,
Pubmed Yatani,
Direct activation of mammalian atrial muscarinic potassium channels by GTP regulatory protein Gk.
1987,
Pubmed Yatani,
The G protein-gated atrial K+ channel is stimulated by three distinct Gi alpha-subunits.
1988,
Pubmed
