Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A leucine residue at position 370 (L370) in 29-4 Shaker K+ channels resides within two overlapping sequence motifs conserved among most voltage-gated channels: the S4 segment and a leucine heptad repeat. Here we investigate the effects observed upon substitution of L370 with many other uncharged amino acid residues. We find that smaller or more hydrophilic residues produce greater alterations in both activation and inactivation gating than does substitution with other large hydrophobic residues. In addition, subunits containing less conservative substitutions at position 370 are restricted in their assembly with wild-type subunits and are unlikely to form homomultimeric channel complexes. Consistent with the idea that L370 influences the tertiary structure of these channels, the results indicate that L370 undergoes specific hydrophobic interactions during the conformational transitions of gating; similar interactions may take place during the folding, insertion, or assembly of Shaker K+ channel subunits.
Abel,
Gene regulation. Action of leucine zippers.
1989, Pubmed
Abel,
Gene regulation. Action of leucine zippers.
1989,
Pubmed Auld,
A neutral amino acid change in segment IIS4 dramatically alters the gating properties of the voltage-dependent sodium channel.
1990,
Pubmed
,
Xenbase Bezanilla,
Molecular basis of gating charge immobilization in Shaker potassium channels.
1991,
Pubmed
,
Xenbase Bowie,
Deciphering the message in protein sequences: tolerance to amino acid substitutions.
1990,
Pubmed Butler,
A family of putative potassium channel genes in Drosophila.
1989,
Pubmed Cess,
Interpretation of cloud-climate feedback as produced by 14 atmospheric general circulation models.
1989,
Pubmed Engelman,
Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins.
1986,
Pubmed Eriksson,
Response of a protein structure to cavity-creating mutations and its relation to the hydrophobic effect.
1992,
Pubmed Go,
Relationship between mutability, polarity and exteriority of amino acid residues in protein evolution.
1980,
Pubmed Hartmann,
Exchange of conduction pathways between two related K+ channels.
1991,
Pubmed
,
Xenbase Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase Isacoff,
Putative receptor for the cytoplasmic inactivation gate in the Shaker K+ channel.
1991,
Pubmed
,
Xenbase Iverson,
The role of the divergent amino and carboxyl domains on the inactivation properties of potassium channels derived from the Shaker gene of Drosophila.
1990,
Pubmed
,
Xenbase Landschulz,
The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite.
1989,
Pubmed Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed Liman,
Voltage-sensing residues in the S4 region of a mammalian K+ channel.
1991,
Pubmed
,
Xenbase Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase Logothetis,
Incremental reductions of positive charge within the S4 region of a voltage-gated K+ channel result in corresponding decreases in gating charge.
1992,
Pubmed
,
Xenbase MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase McCormack,
A role for hydrophobic residues in the voltage-dependent gating of Shaker K+ channels.
1991,
Pubmed
,
Xenbase McCormack,
Tandem linkage of Shaker K+ channel subunits does not ensure the stoichiometry of expressed channels.
1992,
Pubmed
,
Xenbase McCormack,
Shaker K+ channel subunits from heteromultimeric channels with novel functional properties.
1990,
Pubmed
,
Xenbase Mendel,
Probing protein stability with unnatural amino acids.
1992,
Pubmed Noda,
Existence of distinct sodium channel messenger RNAs in rat brain.
,
Pubmed O'Shea,
X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil.
1991,
Pubmed Papazian,
Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence.
1991,
Pubmed
,
Xenbase Rees,
Hydrophobic organization of membrane proteins.
1989,
Pubmed Schoppa,
The size of gating charge in wild-type and mutant Shaker potassium channels.
1992,
Pubmed Stühmer,
Gating currents of inactivating and non-inactivating potassium channels expressed in Xenopus oocytes.
1991,
Pubmed
,
Xenbase Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase Tanabe,
Primary structure of the receptor for calcium channel blockers from skeletal muscle.
,
Pubmed Tempel,
Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila.
1987,
Pubmed Tytgat,
Evidence for cooperative interactions in potassium channel gating.
1992,
Pubmed
,
Xenbase Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed Yool,
Alteration of ionic selectivity of a K+ channel by mutation of the H5 region.
1991,
Pubmed
,
Xenbase
