Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-22305
Biochim Biophys Acta 1993 Aug 18;11782:141-5.
Show Gene links Show Anatomy links

Expression of Na/Pi cotransport from opossum kidney cells in Xenopus laevis oocytes.

Sorribas V, Markovich D, Werner A, Biber J, Murer H.


???displayArticle.abstract???
Xenopus laevis oocytes have been used for the expression of Na/Pi-cotransport activity by injections of poly(A)+ RNA (mRNA) isolated from an established renal cell line (OK cells). 3-5 days after mRNA injection, Na-dependent phosphate (Pi) uptake by oocytes was increased in a dose-dependent manner; there was no increase in Na-independent Pi uptake. Sucrose density-gradient fractionation indicated that the mRNA species encoding this activity is 2.4-2.8 kb in length. In Northern blots, using a cDNA probe related to human kidney-cortex Na/Pi-cotransport activity (NaPi-3), hybridization with a mRNA-species of 2.4-2.6 kb was obtained. Kinetic characterization ([Pi], [Na]) showed that expressed transport activity has properties similar to apical Na/Pi cotransport in OK cells.

???displayArticle.pubmedLink??? 8102252
???displayArticle.link??? Biochim Biophys Acta