Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Localization of NaPi-1, a Na-Pi cotransporter, in rabbit kidneyproximal tubules. I. mRNA localization by reverse transcription/polymerase chain reaction.
Custer M, Meier F, Schlatter E, Greger R, Garcia-Perez A, Biber J, Murer H.
???displayArticle.abstract???
We have recently isolated from a rabbit cortex cDNA library a cDNA clone (NaPi-1), which, after in vitro transcription (cRNA) and injection into Xenopus laevis oocytes, expresses Na-dependent Pi uptake [Werner A, et al. (1991) Proc Natl Acad Sci USA 88:9608-9612]. The aim of the present work was to study the nephron location of the NaPi-1-related mRNA(s) by combining nephron microdissection procedures, reverse transcription (RT) and amplification of the resultant cDNA by the polymerase chain reaction (PCR). RT-PCR using NaPi-1-specific primers (different combinations) and either total kidney cortex RNA or microdissected proximal tubule segments resulted in two PCR products, both of approximately the expected length (but differing by about 30 base pairs). Restriction-enzyme analysis and nucleotide sequencing confirmed that both PCR products are related to NaPi-1 and that the "longer" PCR product has an insert of 26 base pairs containing an AluI restriction site. Nephron microdissection documents expression of NaPi-1-related mRNA(s) in superficial and deep proximal tubules (S1, S2 and S3 segments) and their absence in glomeruli, thin descending limb and thick ascending limbs of Henle's loop, distal convoluted tubules and cortical and inner medullary collecting ducts. These experiments suggest a "microheterogeneity" of NaPi-1-related mRNA(s) (which is not detected in Northern blot analysis) and proximal tubular expression of NaPi-1.
Biber,
Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry.
1993, Pubmed
Biber,
Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry.
1993,
Pubmed Biber,
Cellular aspects of proximal tubular phosphate reabsorption.
1989,
Pubmed Bonjour,
Phosphate transport in the kidney.
1984,
Pubmed Garcia-Perez,
Molecular cloning of cDNA coding for kidney aldose reductase. Regulation of specific mRNA accumulation by NaCl-mediated osmotic stress.
1989,
Pubmed Gmaj,
Cellular mechanisms of inorganic phosphate transport in kidney.
1986,
Pubmed Igarashi,
Molecular biology of renal Na(+)-H+ exchangers.
1991,
Pubmed Krapf,
Na/H antiporter mRNA expression in single nephron segments of rat kidney cortex.
1991,
Pubmed Magaldi,
Phosphate transport in isolated rat inner medullary collecting duct.
1992,
Pubmed Magosci,
Localization of mRNAs coding for isozymes of plasma membrane Ca(2+)-ATPase pump in rat kidney.
1992,
Pubmed Moriyama,
Detection of specific mRNAs in single nephron segments by use of the polymerase chain reaction.
1990,
Pubmed Murer,
Cellular mechanisms in proximal tubular reabsorption of inorganic phosphate.
1991,
Pubmed
,
Xenbase Murer,
Homer Smith Award. Cellular mechanisms in proximal tubular Pi reabsorption: some answers and more questions.
1992,
Pubmed Reilly,
cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells.
1991,
Pubmed Reshkin,
Functional asymmetry of phosphate transport and its regulation in opossum kidney cells: phosphate transport.
1990,
Pubmed Schwab,
Na+ gradient-dependent Pi uptake in basolateral membrane vesicles from dog kidney.
1984,
Pubmed Silbernagl,
The renal handling of amino acids and oligopeptides.
1988,
Pubmed Stevens,
Intestinal transport of amino acids and sugars: advances using membrane vesicles.
1984,
Pubmed Terada,
RT-PCR microlocalization of mRNA for guanylyl cyclase-coupled ANF receptor in rat kidney.
1991,
Pubmed Terubayashi,
Localization of aldose and aldehyde reductase in the kidney.
1989,
Pubmed Tse,
Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.
1991,
Pubmed Tse,
Cloning and sequencing of a rabbit cDNA encoding an intestinal and kidney-specific Na+/H+ exchanger isoform (NHE-3).
1992,
Pubmed Wehrle,
Characteristics of phosphate uptake by Ehrlich ascites tumor cells.
1982,
Pubmed Werner,
Expression of renal transport systems for inorganic phosphate and sulfate in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase Yu,
Identification and localization of renal Na(+)-Ca2+ exchanger by polymerase chain reaction.
1992,
Pubmed Yu,
Molecular characterization and nephron distribution of a family of transcripts encoding the pore-forming subunit of Ca2+ channels in the kidney.
1992,
Pubmed Zimmermann,
A simplified protocol for fast plasmid DNA sequencing.
1990,
Pubmed
