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The mouse one P-domain (pS2) and two P-domain (mSP) genes exhibit distinct patterns of expression.
Lefebvre O, Wolf C, Kédinger M, Chenard MP, Tomasetto C, Chambon P, Rio MC.
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We have previously shown that the human pS2 gene, which codes for a secreted peptide of 60 amino acids, is expressed in a number of human carcinomas, including carcinomas of the breast, the pancreas, and the large bowel. Strong pS2 gene expression was also observed in the normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of the gastrointestinal tract. A number of pS2 similar peptides, designated as P-domain peptides, have been described, notably the porcine (PSP), murine (mSP), and human (hSP) spasmolytic polypeptides, which correspond to duplicated pS2 proteins. We have now cloned a mouse homolog of the human pS2 cDNA to dispose of an animal model to study the pS2 protein function, which remains unknown at the present time. We show that the mouse putative pS2 protein sequence and the physiological pattern of expression of the mouse pS2 gene are well conserved. The mouse pS2 gene is highly expressed in the stomach mucosa cells, whereas no pS2 gene expression could be detected in the mouse mammary gland, even during postnatal development processes dependent on growth factors or hormones. Using in situ hybridization, we show that although coexpressed in the fundus, the antrum and the antrum-pyloric regions of the stomach, the mouse pS2 and mSP genes exhibit distinct and complementary cellular patterns of expression.
Baker,
Oestrogen-induced pS2 protein is similar to pancreatic spasmolytic polypeptide and the kringle domain.
1988, Pubmed
Baker,
Oestrogen-induced pS2 protein is similar to pancreatic spasmolytic polypeptide and the kringle domain.
1988,
Pubmed Berry,
Estrogen-responsive element of the human pS2 gene is an imperfectly palindromic sequence.
1989,
Pubmed Carr,
1H NMR-based determination of the secondary structure of porcine pancreatic spasmolytic polypeptide: one of a new family of "trefoil" motif containing cell growth factors.
1992,
Pubmed Ceriani,
Levels of expression of breast epithelial mucin detected by monoclonal antibody BrE-3 in breast-cancer prognosis.
1992,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Coleman,
Ductal morphogenesis in the mouse mammary gland: evidence supporting a role for epidermal growth factor.
1988,
Pubmed Devine,
Mucins: structure, function, and associations with malignancy.
1992,
Pubmed Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Foekens,
Prediction of relapse and survival in breast cancer patients by pS2 protein status.
1990,
Pubmed Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed Guérin,
Strong association between c-myb and oestrogen-receptor expression in human breast cancer.
1990,
Pubmed Hähnel,
Detection of estradiol-induced messenger RNA (pS2) in uninvolved breast tissue from mastectomies for breast cancer.
1992,
Pubmed Hauser,
xP2, a new member of the P-domain peptide family of potential growth factors, is synthesized in Xenopus laevis skin.
1992,
Pubmed
,
Xenbase Hauser,
xP1 and xP4. P-domain peptides expressed in Xenopus laevis stomach mucosa.
1991,
Pubmed
,
Xenbase Henry,
Expression of the oestrogen regulated pNR-2 mRNA in human breast cancer: relation to oestrogen receptor mRNA levels and response to tamoxifen therapy.
1990,
Pubmed Henry,
Expression of the pNR-2/pS2 protein in diverse human epithelial tumours.
1991,
Pubmed Henry,
pNR-2/pS2 immunohistochemical staining in breast cancer: correlation with prognostic factors and endocrine response.
1991,
Pubmed Jakowlew,
Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7.
1984,
Pubmed Jørgensen,
Pancreatic spasmolytic polypeptide (PSP): III. Pharmacology of a new porcine pancreatic polypeptide with spasmolytic and gastric acid secretion inhibitory effects.
1982,
Pubmed Lan,
Cloning and sequencing of a human pancreatic tumor mucin cDNA.
1990,
Pubmed Luqmani,
Expression of the pS2 gene in normal, benign and neoplastic human stomach.
1989,
Pubmed Masiakowski,
Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line.
1982,
Pubmed McKenzie,
Mucins in breast cancer: recent immunological advances.
1990,
Pubmed Merlo,
Frequent alteration of the DF3 tumor-associated antigen gene in primary human breast carcinomas.
1989,
Pubmed Nunez,
The 5' flanking region of the pS2 gene contains a complex enhancer region responsive to oestrogens, epidermal growth factor, a tumour promoter (TPA), the c-Ha-ras oncoprotein and the c-jun protein.
1989,
Pubmed Piggott,
Antipeptide antibodies against the pNR-2 oestrogen-regulated protein of human breast cancer cells and detection of pNR-2 expression in normal tissues by immunohistochemistry.
1991,
Pubmed Predine,
Enzyme-linked immunosorbent assay of pS2 in breast cancers, benign tumors, and normal breast tissues. Correlation with prognosis and adjuvant hormone therapy.
1992,
Pubmed Rasmussen,
Immunohistochemical localization of pancreatic spasmolytic polypeptide (PSP) in the pig.
1992,
Pubmed Rio,
Breast cancer-associated pS2 protein: synthesis and secretion by normal stomach mucosa.
1988,
Pubmed Rio,
Specific expression of the pS2 gene in subclasses of breast cancers in comparison with expression of the estrogen and progesterone receptors and the oncogene ERBB2.
1987,
Pubmed Rio,
[Primary structure of human protein pS2].
1988,
Pubmed Rio,
The pS2 gene, mRNA, and protein: a potential marker for human breast cancer.
1990,
Pubmed Rio,
Induction of pS2 and hSP genes as markers of mucosal ulceration of the digestive tract.
1991,
Pubmed Robinson,
Regulated expression and growth inhibitory effects of transforming growth factor-beta isoforms in mouse mammary gland development.
1991,
Pubmed Schwartz,
pS2 expression and response to hormonal therapy in patients with advanced breast cancer.
1991,
Pubmed Seitz,
Breast cancer-associated protein pS2 expression in tumors of the biliary tract.
1991,
Pubmed Skilton,
Characterisation of a messenger RNA selectively expressed in human breast cancer.
1989,
Pubmed Snedeker,
Expression and functional properties of transforming growth factor alpha and epidermal growth factor during mouse mammary gland ductal morphogenesis.
1991,
Pubmed Spicer,
Molecular cloning and analysis of the mouse homologue of the tumor-associated mucin, MUC1, reveals conservation of potential O-glycosylation sites, transmembrane, and cytoplasmic domains and a loss of minisatellite-like polymorphism.
1991,
Pubmed Suemori,
Identification and characterization of rat intestinal trefoil factor: tissue- and cell-specific member of the trefoil protein family.
1991,
Pubmed Theisinger,
Expression of the breast cancer associated gene pS2 and the pancreatic spasmolytic polypeptide gene (hSP) in diffuse type of stomach carcinoma.
1991,
Pubmed Thim,
A surprising sequence homology.
1988,
Pubmed Thim,
A new family of growth factor-like peptides. 'Trefoil' disulphide loop structures as a common feature in breast cancer associated peptide (pS2), pancreatic spasmolytic polypeptide (PSP), and frog skin peptides (spasmolysins).
1989,
Pubmed
,
Xenbase Thim,
The amino acid sequence of pancreatic spasmolytic polypeptide.
1985,
Pubmed Tomasetto,
hSP, the domain-duplicated homolog of pS2 protein, is co-expressed with pS2 in stomach but not in breast carcinoma.
1990,
Pubmed Topper,
Multiple hormone interactions in the developmental biology of the mammary gland.
1980,
Pubmed von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed Wright,
Induction of a novel epidermal growth factor-secreting cell lineage by mucosal ulceration in human gastrointestinal stem cells.
1990,
Pubmed Wright,
Epidermal growth factor (EGF/URO) induces expression of regulatory peptides in damaged human gastrointestinal tissues.
1990,
Pubmed Wysocki,
Hormone-sensitive gene expression in breast tumours.
1990,
Pubmed
