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Study of multiple fibrillarin mRNAs reveals that 3' end formation in Schizosaccharomyces pombe is sensitive to cold shock.
Girard JP, Feliu J, Caizergues-Ferrer M, Lapeyre B.
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Fibrillarin is a nucleolar protein which is associated with small nucleolar RNAs, and is required for pre-rRNA processing. We have cloned and characterized the gene encoding fibrillarin in the fission yeast Schizosaccharomyces pombe and we have followed its expression under various conditions. Fission yeast fibrillarin is a 305 amino-acid protein which appears to be highly conserved throughout evolution. In Xenopus, human or Saccharomyces cerevisiae, a single fibrillarin mRNA is detected while, in S. pombe a single copy gene encodes different mRNAs which differ at the 3' ends. Under normal growth conditions, two mRNAs of 1.1 and 1.35 kb are detected with the 1.1 kb being the most abundant. Both the total amount and relative abundance of these two mRNAs are strongly affected by exposure to low temperature, namely the 1.1 kb mRNA almost disappears while the 1.35 kb is less markedly diminished. A new species of 3.2 kb accumulates in the cell, which contains an unusually long 3' untranslated region of 2 kb. We have found that exposure of the cells to a cold shock has a profound effect on 3' end formation in S.pombe since the transcription of several other mRNAs is also capable of skipping the normal 3' end site to terminate at a further downstream site.
Amaldi,
Translational regulation of the expression of ribosomal protein genes in Xenopus laevis.
1990, Pubmed,
Xenbase
Amaldi,
Translational regulation of the expression of ribosomal protein genes in Xenopus laevis.
1990,
Pubmed
,
Xenbase Aris,
cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera.
1991,
Pubmed
,
Xenbase Bachvarova,
A maternal tail of poly(A): the long and the short of it.
1992,
Pubmed Bally,
SnR30: a new, essential small nuclear RNA from Saccharomyces cerevisiae.
1988,
Pubmed Caizergues-Ferrer,
Nucleolin from Xenopus laevis: cDNA cloning and expression during development.
1989,
Pubmed
,
Xenbase Caizergues-Ferrer,
Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis.
1991,
Pubmed
,
Xenbase Dandekar,
Cloning of Schizosaccharomyces pombe genes encoding the U1, U2, U3 and U4 snRNAs.
1989,
Pubmed de Sauvage,
Alternative polyadenylation of the amyloid protein precursor mRNA regulates translation.
1992,
Pubmed
,
Xenbase Eng,
Structural basis for the regulation of splicing of a yeast messenger RNA.
1991,
Pubmed Fantes,
Epistatic gene interactions in the control of division in fission yeast.
1979,
Pubmed Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed Gatermann,
A ribosomal protein gene family from Schizosaccharomyces pombe consisting of three active members.
1989,
Pubmed Girard,
GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast.
1992,
Pubmed Grimm,
Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.
1988,
Pubmed Henríquez,
Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen.
1990,
Pubmed Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed Hughes,
The yeast homologue of U3 snRNA.
1987,
Pubmed
,
Xenbase Humphrey,
Homologous mRNA 3' end formation in fission and budding yeast.
1991,
Pubmed Irniger,
Different classes of polyadenylation sites in the yeast Saccharomyces cerevisiae.
1991,
Pubmed Jacob,
Polyadenylation of mRNA and its control.
1990,
Pubmed Jansen,
Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.
1991,
Pubmed
,
Xenbase Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed Kenan,
RNA recognition: towards identifying determinants of specificity.
1991,
Pubmed Lapeyre,
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
1990,
Pubmed
,
Xenbase Lapeyre,
Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence.
1987,
Pubmed Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed Maicas,
The accumulation of three yeast ribosomal proteins under conditions of excess mRNA is determined primarily by fast protein decay.
1988,
Pubmed Manley,
RNA 3'-end formation.
1989,
Pubmed Mayer,
Yeast CBP1 mRNA 3' end formation is regulated during the induction of mitochondrial function.
1991,
Pubmed Moreno,
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
1991,
Pubmed Porter,
The sequence of U3 from Schizosaccharomyces pombe suggests structural divergence of this snRNA between metazoans and unicellular eukaryotes.
1988,
Pubmed Potashkin,
Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.
1990,
Pubmed Presutti,
The ribosomal protein L2 in S. cerevisiae controls the level of accumulation of its own mRNA.
1991,
Pubmed Raué,
Ribosome biogenesis in yeast.
1991,
Pubmed Russell,
cdc25+ functions as an inducer in the mitotic control of fission yeast.
1986,
Pubmed Sachs,
The role of poly(A) in the translation and stability of mRNA.
1990,
Pubmed Saiki,
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
1988,
Pubmed Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed Thekkumkara,
Use of alternative polyadenylation sites for tissue-specific transcription of two angiotensin-converting enzyme mRNAs.
1992,
Pubmed Tollervey,
Immunoprecipitation distinguishes non-overlapping groups of snRNPs in Schizosaccharomyces pombe.
1990,
Pubmed Tollervey,
A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
1987,
Pubmed Tollervey,
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
1991,
Pubmed Tsay,
Ribosomal protein synthesis is not regulated at the translational level in Saccharomyces cerevisiae: balanced accumulation of ribosomal proteins L16 and rp59 is mediated by turnover of excess protein.
1988,
Pubmed Wang,
Choice of 3' cleavage/polyadenylation site in beta-tropomyosin RNA processing is differentiation-dependent in mouse BC3H1 muscle cells.
1992,
Pubmed Warner,
Synthesis of ribosomes in Saccharomyces cerevisiae.
1989,
Pubmed Warner,
The nucleolus and ribosome formation.
1990,
Pubmed Warner,
Saccharomyces cerevisiae coordinates accumulation of yeast ribosomal proteins by modulating mRNA splicing, translational initiation, and protein turnover.
1985,
Pubmed Wickens,
How the messenger got its tail: addition of poly(A) in the nucleus.
1990,
Pubmed Zagorski,
Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.
1988,
Pubmed Zaret,
DNA sequence required for efficient transcription termination in yeast.
1982,
Pubmed
