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A Xenopus 45 kDa protein has been identified as an immediate upstream factor sufficient for full activation of MAP kinase, and is shown to be capable of undergoing autophosphorylation on serine, threonine and tyrosine residues. In this study, we show that purified 45 kDa protein can phosphorylate a kinase-negative mutant of Xenopus MAP kinase on tyrosine and threonine residues, suggesting that the 45 kDa protein functions as a MAP kinase kinase to activate MAP kinase. We then report the cloning and sequencing of a full-length cDNA encoding this 45 kDa MAP kinase kinase, and show that it is highly homologous to four protein kinases in fission and budding yeasts: byr1, wis1, PBS2 and STE7. These yeast kinases are therefore suggested to function as a direct upstream activator for a presumed MAP kinase homolog in each signal transduction pathway involved in the regulation of cell cycle progression or cellular responses to extracellular signals. Finally, we report bacterial expression of recombinant MAP kinase kinase that can be phosphorylated and activated by Xenopus egg extracts.
Ahn,
Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.
1991, Pubmed
Ahn,
Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.
1991,
Pubmed Alvarez,
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase.
1991,
Pubmed Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed Boguslawski,
Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases.
1987,
Pubmed Boulton,
ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.
1991,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Cobb,
Extracellular signal-regulated kinases: ERKs in progress.
1991,
Pubmed Courchesne,
A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae.
1989,
Pubmed Crews,
Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine.
1991,
Pubmed Elion,
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
1990,
Pubmed Fukui,
Role of a ras homolog in the life cycle of Schizosaccharomyces pombe.
1986,
Pubmed Fukui,
Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe.
1985,
Pubmed Gómez,
Dissection of the protein kinase cascade by which nerve growth factor activates MAP kinases.
1991,
Pubmed Gotoh,
Okadaic acid activates microtubule-associated protein kinase in quiescent fibroblastic cells.
1990,
Pubmed Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase Gotoh,
Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
1990,
Pubmed Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed Hattori,
Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system.
1992,
Pubmed
,
Xenbase Hoshi,
Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.
1988,
Pubmed Kosako,
Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation.
1992,
Pubmed
,
Xenbase Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed Lee,
Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog.
1992,
Pubmed Leevers,
Activation of extracellular signal-regulated kinase, ERK2, by p21ras oncoprotein.
1992,
Pubmed Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed
,
Xenbase Nadin-Davis,
A gene which encodes a predicted protein kinase can restore some functions of the ras gene in fission yeast.
1988,
Pubmed Nakielny,
MAP kinase activator from insulin-stimulated skeletal muscle is a protein threonine/tyrosine kinase.
1992,
Pubmed Nishida,
Mitogen-activated protein kinase and cytoskeleton in mitogenic signal transduction.
1992,
Pubmed Payne,
Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).
1991,
Pubmed Pelech,
MAP kinases: charting the regulatory pathways.
1992,
Pubmed Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Pulverer,
Phosphorylation of c-jun mediated by MAP kinases.
1991,
Pubmed Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed Rhodes,
STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.
1990,
Pubmed Seger,
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.
1991,
Pubmed Shirakabe,
A mitogen-activated protein (MAP) kinase activating factor in mammalian mitogen-stimulated cells is homologous to Xenopus M phase MAP kinase activator.
1992,
Pubmed
,
Xenbase Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase Sturgill,
Recent progress in characterization of protein kinase cascades for phosphorylation of ribosomal protein S6.
1991,
Pubmed Teague,
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.
1986,
Pubmed Thomas,
Ras is essential for nerve growth factor- and phorbol ester-induced tyrosine phosphorylation of MAP kinases.
1992,
Pubmed Toda,
Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.
1991,
Pubmed Torres,
A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants.
1991,
Pubmed Wang,
byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype.
1991,
Pubmed Warbrick,
The wis1 protein kinase is a dosage-dependent regulator of mitosis in Schizosaccharomyces pombe.
1991,
Pubmed Wood,
ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSK.
1992,
Pubmed Wu,
Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.
1991,
Pubmed
