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We describe the transcriptional potentiation in estrogen responsive transcription extracts of the Xenopus vitellogenin B1 gene promoter through the formation of a positioned nucleosome. Nuclease digestion and hydroxyl radical cleavage indicate that strong, DNA sequence-directed positioning of a nucleosome occurs between -300 and -140 relative to the start site of transcription. Deletion of this DNA sequence abolishes the potentiation of transcription due to nucleosome assembly. The wrapping of DNA around the histone core of the nucleosome positioned between -300 and -140 creates a static loop in which distal estrogen receptor binding sites are brought close to proximal promoter elements. This might facilitate interactions between the trans-acting factors themselves and/or RNA polymerase. Such a nucleosome provides an example of how chromatin structure might have a positive effect on the transcription process.
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991, Pubmed
Archer,
Transcription factor access is mediated by accurately positioned nucleosomes on the mouse mammary tumor virus promoter.
1991,
Pubmed Burch,
Chromatin structural transitions and the phenomenon of vitellogenin gene memory in chickens.
1986,
Pubmed Burch,
Chromatin studies reveal that an ERE is located far upstream of a vitellogenin gene and that a distal tissue-specific hypersensitive site is conserved for two coordinately regulated vitellogenin genes.
1990,
Pubmed Burch,
Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
1983,
Pubmed Camerini-Otero,
The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
1976,
Pubmed Chang,
An NF1-related vitellogenin activator element mediates transcription from the estrogen-regulated Xenopus laevis vitellogenin promoter.
1990,
Pubmed
,
Xenbase Chipev,
Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo.
1992,
Pubmed
,
Xenbase Clark,
Superhelical stress and nucleosome-mediated repression of 5S RNA gene transcription in vitro.
1991,
Pubmed
,
Xenbase Corthésy,
Estrogen receptor level determines sex-specific in vitro transcription from the Xenopus vitellogenin promoter.
1990,
Pubmed
,
Xenbase Corthésy,
A liver protein fraction regulating hormone-dependent in vitro transcription from the vitellogenin genes induces their expression in Xenopus oocytes.
1991,
Pubmed
,
Xenbase Corthésy,
A nuclear factor I-like activity and a liver-specific repressor govern estrogen-regulated in vitro transcription from the Xenopus laevis vitellogenin B1 promoter.
1989,
Pubmed
,
Xenbase Corthésy,
Estrogen-dependent in vitro transcription from the vitellogenin promoter in liver nuclear extracts.
1988,
Pubmed
,
Xenbase Corthésy,
Transcriptional potentiation of the vitellogenin B1 promoter by a combination of both nucleosome assembly and transcription factors: an in vitro dissection.
1990,
Pubmed
,
Xenbase Döbbeling,
A cell-specific activator in the Xenopus A2 vitellogenin gene: promoter elements functioning with rat liver nuclear extracts.
1988,
Pubmed
,
Xenbase Elgin,
The formation and function of DNase I hypersensitive sites in the process of gene activation.
1988,
Pubmed Fascher,
Role of trans-activating proteins in the generation of active chromatin at the PHO5 promoter in S. cerevisiae.
1990,
Pubmed Felsenfeld,
Chromatin as an essential part of the transcriptional mechanism.
1992,
Pubmed FitzGerald,
Effects of sequence alterations in a DNA segment containing the 5 S RNA gene from Lytechinus variegatus on positioning of a nucleosome core particle in vitro.
1985,
Pubmed Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed Hayes,
The interaction of transcription factors with nucleosomal DNA.
1992,
Pubmed Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase Hayes,
The histone core exerts a dominant constraint on the structure of DNA in a nucleosome.
1991,
Pubmed Imai,
A simple and rapid method for generating a deletion by PCR.
1991,
Pubmed Iyer,
Chicken vitellogenin gene-binding protein, a leucine zipper transcription factor that binds to an important control element in the chicken vitellogenin II promoter, is related to rat DBP.
1991,
Pubmed
,
Xenbase Jayasena,
Competitive nucleosome reconstitution of polydeoxynucleotides containing oligoguanosine tracts.
1989,
Pubmed Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase Kumar,
The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer.
1988,
Pubmed Luckow,
CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements.
1987,
Pubmed Lutter,
Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix.
1978,
Pubmed Martinez,
Synergistic transcriptional activation by CTF/NF-I and the estrogen receptor involves stabilized interactions with a limiting target factor.
1991,
Pubmed Perlmann,
Specific glucocorticoid receptor binding to DNA reconstituted in a nucleosome.
1988,
Pubmed Philipsen,
In vivo footprinting of the estrogen-inducible vitellogenin II gene from chicken.
1988,
Pubmed Piña,
Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter.
1990,
Pubmed Reik,
Glucocorticoids are required for establishment and maintenance of an alteration in chromatin structure: induction leads to a reversible disruption of nucleosomes over an enhancer.
1991,
Pubmed Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase Schmid,
More than just "histone-like" proteins.
1990,
Pubmed Seal,
Mutational studies reveal a complex set of positive and negative control elements within the chicken vitellogenin II promoter.
1991,
Pubmed Seiler-Tuyns,
Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
1986,
Pubmed
,
Xenbase Shrader,
Artificial nucleosome positioning sequences.
1989,
Pubmed Simon,
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
1979,
Pubmed Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed Simpson,
Nucleosome positioning: occurrence, mechanisms, and functional consequences.
1991,
Pubmed Straka,
A functional role for nucleosomes in the repression of a yeast promoter.
1991,
Pubmed Theulaz,
Expression of human estrogen receptor mutants in Xenopus oocytes: correlation between transcriptional activity and ability to form protein-DNA complexes.
1988,
Pubmed
,
Xenbase Thoma,
Local protein-DNA interactions may determine nucleosome positions on yeast plasmids.
,
Pubmed Thomas,
Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.
1988,
Pubmed Wahli,
Evolution and expression of vitellogenin genes.
1988,
Pubmed Walker,
Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
1984,
Pubmed
,
Xenbase Wolffe,
Initiation of transcription on nucleosomal templates.
1989,
Pubmed Wolffe,
Activating chromatin.
1991,
Pubmed Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase Wolffe,
Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro.
1988,
Pubmed
,
Xenbase Wolffe,
Chromatin assembly.
1991,
Pubmed
,
Xenbase Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
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