Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1992 Nov 15;8922:10822-6.
Show Gene links
Show Anatomy links
Definition of the binding sites of individual zinc fingers in the transcription factor IIIA-5S RNA gene complex.
Clemens KR, Liao X, Wolf V, Wright PE, Gottesfeld JM.
???displayArticle.abstract???
A series of polypeptides containing increasing numbers of zinc fingers of Xenopus transcription factor IIIA has been generated and binding to the 5S RNA gene internal control region has been studied in order to elucidate the mode of interaction of the individual fingers with DNA. By using a combination of DNase I footprinting, methylation interference, and differential binding to mixtures of DNA fragments differing in length by single base pairs, the binding sites for individual fingers have been defined. These results have led to a model for the interaction of transcription factor IIIA with the internal control region in which fingers 1-3 bind in the major groove of the promoter C block, fingers 7-9 bind in the major groove of the A block, and finger 5 binds in the major groove of the intermediate element. Fingers 4 and 6 each bind across the minor groove, spanning these promoter elements.
Berg,
Zinc finger domains: hypotheses and current knowledge.
1990, Pubmed
Berg,
Zinc finger domains: hypotheses and current knowledge.
1990,
Pubmed Churchill,
Mode of interaction of the zinc finger protein TFIIIA with a 5S RNA gene of Xenopus.
1990,
Pubmed
,
Xenbase Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Fairall,
Mapping of the sites of protection on a 5 S RNA gene by the Xenopus transcription factor IIIA. A model for the interaction.
1986,
Pubmed
,
Xenbase Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase Gottesfeld,
DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.
1987,
Pubmed
,
Xenbase Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase Kochoyan,
Alternating zinc fingers in the human male associated protein ZFY: 2D NMR structure of an even finger and implications for "jumping-linker" DNA recognition.
1991,
Pubmed Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed Liao,
Specific interaction of the first three zinc fingers of TFIIIA with the internal control region of the Xenopus 5 S RNA gene.
1992,
Pubmed
,
Xenbase Liu,
Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis.
1990,
Pubmed Liu-Johnson,
The DNA binding domain and bending angle of E. coli CAP protein.
1986,
Pubmed MacFerrin,
Overproduction and dissection of proteins by the expression-cassette polymerase chain reaction.
1990,
Pubmed McLeod,
The product of the mei3+ gene, expressed under control of the mating-type locus, induces meiosis and sporulation in fission yeast.
1987,
Pubmed Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase Pavletich,
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
1991,
Pubmed Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase Pieler,
The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing.
1987,
Pubmed
,
Xenbase Reynolds,
Torsional stress induces an S1 nuclease-hypersensitive site within the promoter of the Xenopus laevis oocyte-type 5S RNA gene.
1985,
Pubmed
,
Xenbase Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase Romaniuk,
Characterization of the equilibrium binding of Xenopus transcription factor IIIA to the 5 S RNA gene.
1990,
Pubmed
,
Xenbase Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase Sands,
The carboxyterminal zinc fingers of TFIIIA interact with the tip of helix V of 5S RNA in the 7S ribonucleoprotein particle.
1991,
Pubmed
,
Xenbase Schroth,
Transcription factor IIIA induced bending of the Xenopus somatic 5S gene promoter.
1989,
Pubmed
,
Xenbase Schroth,
TFIIIA induced DNA bending: effect of low ionic strength electrophoresis buffer conditions.
1991,
Pubmed
,
Xenbase Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase Studier,
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
1986,
Pubmed Tso,
Structure of the gene for Xenopus transcription factor TFIIIA.
1986,
Pubmed
,
Xenbase Van Dyke,
Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).
1982,
Pubmed Vrana,
Mapping functional regions of transcription factor TFIIIA.
1988,
Pubmed
,
Xenbase Xing,
The C-terminal domain of transcription factor IIIA interacts differently with different 5S RNA genes.
1989,
Pubmed
,
Xenbase Yasui,
Presence of multiple species of polypeptides immunologically related to transcription factor TFIIIA in adult Xenopus tissues.
1989,
Pubmed
,
Xenbase You,
Mutations in 5S DNA and 5S RNA have different effects on the binding of Xenopus transcription factor IIIA.
1991,
Pubmed
,
Xenbase
