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Proc Natl Acad Sci U S A
1992 Nov 15;8922:10955-9. doi: 10.1073/pnas.89.22.10955.
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Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain.
Storck T, Schulte S, Hofmann K, Stoffel W.
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Transport systems specific for L-glutamate and L-aspartate play an important role in the termination of neurotransmitter signals at excitatory synapses. We describe here the structure and function of a 66-kDa glycoprotein that was purified from rat brain and identified as an L-glutamate/L-aspartate transporter (GLAST). A GLAST-specific cDNA clone was isolated from a rat brain cDNA library. The cDNA insert encodes a polypeptide with 543 amino acid residues (59,697 Da). The amino acid sequence of GLAST suggests a distinctive structure and membrane topology, with some conserved motifs also present in prokaryotic glutamate transporters. The transporter function has been verified by amino acid uptake studies in the Xenopus laevis oocyte system. GLAST is specific for L-glutamate and L-aspartate, shows strict dependence on Na+ ions, and is inhibited by DL-threo-3-hydroxy-aspartate. In situ hybridization reveals a strikingly high density of GLAST mRNA in the Purkinje cell layer of cerebellum, presumably in the Bergmann glia cells, and a less dense distribution throughout the cerebrum. These data suggest that GLAST may be involved in the regulation of neurotransmitter concentration in central nervous system.
Balcar,
The structural specificity of the high affinity uptake of L-glutamate and L-aspartate by rat brain slices.
1972, Pubmed
Balcar,
The structural specificity of the high affinity uptake of L-glutamate and L-aspartate by rat brain slices.
1972,
Pubmed Blakely,
Cloning and expression of a functional serotonin transporter from rat brain.
1991,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Danbolt,
Purification and reconstitution of the sodium- and potassium-coupled glutamate transport glycoprotein from rat brain.
1990,
Pubmed Deguchi,
Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B.
1990,
Pubmed Drejer,
Characterization of uptake and release processes for D- and L-aspartate in primary cultures of astrocytes and cerebellar granule cells.
1983,
Pubmed Eisenberg,
Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
1984,
Pubmed Flott,
Characterization of glutamate uptake systems in astrocyte primary cultures from rat brain.
1991,
Pubmed Fonnum,
Glutamate: a neurotransmitter in mammalian brain.
1984,
Pubmed Gordon,
Partial purification of the sodium- and potassium-coupled L-glutamate transport glycoprotein from rat brain.
1988,
Pubmed Guastella,
Cloning and expression of a rat brain GABA transporter.
1990,
Pubmed
,
Xenbase Hoffman,
Cloning of a serotonin transporter affected by antidepressants.
1991,
Pubmed Hollmann,
Glutamate transport and not glutamate receptor binding is stimulated by gangliosides in a Ca2+-dependent manner in rat brain synaptic plasma membranes.
1989,
Pubmed Hollmann,
Calcium ions induce glutamate transport into rat brain membrane vesicles in the absence of sodium and chloride. Evidence for a novel uptake site?
1988,
Pubmed Jiang,
Conservation between coding and regulatory elements of Rhizobium meliloti and Rhizobium leguminosarum dct genes.
1989,
Pubmed Kalman,
Characterization of the Escherichia coli K12 gltS glutamate permease gene.
1991,
Pubmed Kilty,
Cloning and expression of a cocaine-sensitive rat dopamine transporter.
1991,
Pubmed Kimelberg,
Excitatory amino acid-stimulated uptake of 22Na+ in primary astrocyte cultures.
1989,
Pubmed Kurth,
A facile method for the isolation and preparation of proteins and peptides for sequence analysis in the picomolar range.
1990,
Pubmed Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed Logan,
Unique high affinity uptake systems for glycine, glutamic and aspartic acids in central nervous tissue of the rat.
1971,
Pubmed McBean,
Neurotoxicity of L-glutamate and DL-threo-3-hydroxyaspartate in the rat striatum.
1985,
Pubmed Monaghan,
The excitatory amino acid receptors: their classes, pharmacology, and distinct properties in the function of the central nervous system.
1989,
Pubmed Okamoto,
Synaptic receptors and intracellular signal transduction in the cerebellum.
1991,
Pubmed Pacholczyk,
Expression cloning of a cocaine- and antidepressant-sensitive human noradrenaline transporter.
1991,
Pubmed Palmer,
Excitatory amino acid-releasing and cholinergic neurones in Alzheimer's disease.
1986,
Pubmed Pin,
The Ca2+/C1- dependent L-[3H]glutamate binding: a new receptor or a particular transport process?
1984,
Pubmed Pines,
Counterflow of L-glutamate in plasma membrane vesicles and reconstituted preparations from rat brain.
1990,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schägger,
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
1987,
Pubmed Shimada,
Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA.
1991,
Pubmed
,
Xenbase Tolner,
Revised nucleotide sequence of the gltP gene, which encodes the proton-glutamate-aspartate transport protein of Escherichia coli K-12.
1992,
Pubmed Usdin,
Cloning of the cocaine-sensitive bovine dopamine transporter.
1991,
Pubmed Waniewski,
Characterization of L-glutamic acid transport by glioma cells in culture: evidence for sodium-independent, chloride-dependent high affinity influx.
1984,
Pubmed Zaczek,
Quisqualate-sensitive, chloride-dependent transport of glutamate into rat brain synaptosomes.
1987,
Pubmed
