Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
1992 Nov 01;311:1279-94. doi: 10.1091/mbc.3.11.1279.
Show Gene links
Show Anatomy links
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
Kobayashi H, Stewart E, Poon R, Adamczewski JP, Gannon J, Hunt T.
???displayArticle.abstract???
The binding of cyclin A to p34cdc2 and p32cdk2 and the protein kinase activity of the complexes has been measured by cell-free translation of the corresponding mRNA in extracts of frog eggs, followed by immunoprecipitation. A variety of mutant cyclin A molecules have been constructed and tested in this assay. Small deletions and point mutations of highly conserved residues in the 100-residue "cyclin box" abolish binding and activation of both p34cdc2 and p32cdk2. By contrast, large deletions at the N-terminus have no effect on kinase binding and activation, until they remove residues beyond 161, where the first conserved amino acids are found in all known examples of cyclin A. At the C-terminus, removal of 14 or more amino acids abolishes activity. We also demonstrate that deletion of, or point mutations, in the cyclin A homologue of the 10-residue "destruction box," previously described in cyclin B (Glotzer et al., 1991) abolish cyclin proteolysis at the transition from M-phase to interphase.
Brizuela,
p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.
1987, Pubmed
Brizuela,
p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.
1987,
Pubmed Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Dasso,
Efficient initiation of mammalian mRNA translation at a CUG codon.
1989,
Pubmed Desai,
Activation of human cyclin-dependent kinases in vitro.
1992,
Pubmed Devault,
Cyclin A potentiates maturation-promoting factor activation in the early Xenopus embryo via inhibition of the tyrosine kinase that phosphorylates cdc2.
1992,
Pubmed
,
Xenbase Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed Ducommun,
cdc2 phosphorylation is required for its interaction with cyclin.
1991,
Pubmed
,
Xenbase Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed Elledge,
A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.
1991,
Pubmed
,
Xenbase Evan,
Characterization of the human c-myc protein using antibodies prepared against synthetic peptides.
1986,
Pubmed Fang,
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.
1991,
Pubmed
,
Xenbase Featherstone,
Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase.
1991,
Pubmed Fleig,
Regulation of cdc2 activity in Schizosaccharomyces pombe: the role of phosphorylation.
1991,
Pubmed Gabrielli,
A cdc2-related kinase oscillates in the cell cycle independently of cyclins G2/M and cdc2.
1992,
Pubmed
,
Xenbase Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase Gibbs,
Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions.
1991,
Pubmed Girard,
Cyclin A is required for the onset of DNA replication in mammalian fibroblasts.
1991,
Pubmed Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed Gould,
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
1991,
Pubmed Gu,
Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15.
1992,
Pubmed Hadwiger,
A family of cyclin homologs that control the G1 phase in yeast.
1989,
Pubmed Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase Kobayashi,
Cyclins and their partners during Xenopus oocyte maturation.
1991,
Pubmed
,
Xenbase Kumagai,
Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
1992,
Pubmed
,
Xenbase Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed Lehner,
Drosophila cdc2 homologs: a functional homolog is coexpressed with a cognate variant.
1990,
Pubmed Lehner,
Expression and function of Drosophila cyclin A during embryonic cell cycle progression.
1989,
Pubmed Léopold,
An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins.
1991,
Pubmed Lew,
Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.
1991,
Pubmed Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase Lorca,
Cyclin A-Cys41 does not undergo cell cycle-dependent degradation in Xenopus extracts.
1992,
Pubmed
,
Xenbase Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase Luca,
Control of programmed cyclin destruction in a cell-free system.
1989,
Pubmed Maller,
Mitotic control.
1991,
Pubmed Matsushime,
Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle.
1991,
Pubmed Meyerson,
A family of human cdc2-related protein kinases.
1992,
Pubmed Milarski,
Cloning and characterization of Xenopus cdc2, a component of MPF.
1991,
Pubmed
,
Xenbase Millar,
The cdc25 M-phase inducer: an unconventional protein phosphatase.
1992,
Pubmed Minshull,
Xenopus oocyte maturation does not require new cyclin synthesis.
1991,
Pubmed
,
Xenbase Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed Motokura,
A novel cyclin encoded by a bcl1-linked candidate oncogene.
1991,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Nielsen,
Preparation of capped RNA transcripts using T7 RNA polymerase.
1986,
Pubmed Nugent,
Conserved structural motifs in cyclins identified by sequence analysis.
1991,
Pubmed Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed Pagano,
Cyclin A is required at two points in the human cell cycle.
1992,
Pubmed Paris,
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.
1991,
Pubmed
,
Xenbase Parker,
p107wee1 is a dual-specificity kinase that phosphorylates p34cdc2 on tyrosine 15.
1992,
Pubmed Parker,
Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner.
1991,
Pubmed Pines,
p34cdc2: the S and M kinase?
1990,
Pubmed Pondaven,
Activation of M-phase-specific histone H1 kinase by modification of the phosphorylation of its p34cdc2 and cyclin components.
1990,
Pubmed Roy,
Activation of p34cdc2 kinase by cyclin A.
1991,
Pubmed
,
Xenbase Shamu,
Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase.
1992,
Pubmed
,
Xenbase Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Tsai,
Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.
1991,
Pubmed
,
Xenbase van Loon,
The role of cyclins in the maturation of Patella vulgata oocytes.
1991,
Pubmed Wang,
Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma.
1990,
Pubmed Xiong,
Human D-type cyclin.
1991,
Pubmed Yamashita,
Purification and characterization of maturation-promoting factor in fish.
1992,
Pubmed
,
Xenbase
