Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract??? Cell-free extracts of Xenopus eggs will replicate plasmid DNA molecules under normal cell cycle control. We have used the neutral/neutral 2-D gel technique to map the sites at which DNA replication initiates in this system. Three different plasmids were studied: one containing the Xenopus rDNA repeat, one containing single copy Xenopus genomic DNA, and another containing the yeast 2 microns replication origin. 2-D gel profiles show that many potential sites of initiation are present on each plasmid, and are randomly situated at the level of resolution of this technique (500-1000 bp). Despite the abundance of sites capable of supporting the initiation of replication, pulse-chase experiments suggest that only a single randomly situated initiation event occurs on each DNA molecule. Once initiation has taken place, conventional replication forks appear to move away from this site at a rate of about 10nt/second, similar to the rate observed in vivo.
Bakken,
Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
1982, Pubmed,
Xenbase
Bakken,
Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
1982,
Pubmed
,
Xenbase Blow,
Nuclear structure and the control of DNA replication in the Xenopus embryo.
1989,
Pubmed
,
Xenbase Blow,
A cdc2-like protein is involved in the initiation of DNA replication in Xenopus egg extracts.
1990,
Pubmed
,
Xenbase Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase Blow,
Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.
1987,
Pubmed
,
Xenbase Blow,
Chromosome replication in cell-free systems from Xenopus eggs.
1987,
Pubmed
,
Xenbase Blow,
Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly.
1990,
Pubmed
,
Xenbase Brewer,
A replication fork barrier at the 3' end of yeast ribosomal RNA genes.
1988,
Pubmed Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed Burhans,
Identification of an origin of bidirectional DNA replication in mammalian chromosomes.
1990,
Pubmed Callan,
Replication of DNA in the chromosomes of eukaryotes.
1972,
Pubmed Celniker,
Yeast DNA replication in vitro: initiation and elongation events mimic in vivo processes.
1982,
Pubmed Gaudette,
Replication forks are underrepresented in chromosomal DNA of Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase Hand,
Eucaryotic DNA: organization of the genome for replication.
1978,
Pubmed Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase Heinzel,
Autonomous DNA replication in human cells is affected by the size and the source of the DNA.
1991,
Pubmed Huberman,
The in vivo replication origin of the yeast 2 microns plasmid.
1987,
Pubmed Huberman,
Close association of a DNA replication origin and an ARS element on chromosome III of the yeast, Saccharomyces cerevisiae.
1988,
Pubmed Huberman,
On the mechanism of DNA replication in mammalian chromosomes.
1968,
Pubmed Kearsey,
Structural requirements for the function of a yeast chromosomal replicator.
1984,
Pubmed Krysan,
Isolation of human sequences that replicate autonomously in human cells.
1989,
Pubmed Linskens,
Organization of replication of ribosomal DNA in Saccharomyces cerevisiae.
1988,
Pubmed Linskens,
Ambiguities in results obtained with 2D gel replicon mapping techniques.
1990,
Pubmed McTiernan,
Initiation of SV40 DNA replication after microinjection into Xenopus eggs.
1984,
Pubmed
,
Xenbase Méchali,
Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast.
1984,
Pubmed
,
Xenbase Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase Palzkill,
A yeast replication origin consists of multiple copies of a small conserved sequence.
1988,
Pubmed Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase Stinchcomb,
Isolation and characterisation of a yeast chromosomal replicator.
1979,
Pubmed Umek,
New beginnings in studies of eukaryotic DNA replication origins.
1989,
Pubmed Umek,
The ease of DNA unwinding as a determinant of initiation at yeast replication origins.
1988,
Pubmed Vaughn,
Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain.
1990,
Pubmed Wright,
Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae.
1986,
Pubmed
