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Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel.
Gellens ME, George AL, Chen LQ, Chahine M, Horn R, Barchi RL, Kallen RG.
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The principal voltage-sensitive sodium channel from human heart has been cloned, sequenced, and functionally expressed. The cDNA, designated hH1, encodes a 2016-amino acid protein that is homologous to other members of the sodium channel multigene family and bears greater than 90% identity to the tetrodotoxin-insensitive sodium channel characteristic of rat heart and of immature and denervated rat skeletal muscle. Northern blot analysis demonstrates an approximately 9.0-kilobase transcript expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. When expressed in Xenopus oocytes, hH1 exhibits rapid activation and inactivation kinetics similar to native cardiac sodium channels. The single channel conductance of hH1 to sodium ions is about twice that of the homologous rat channel and hH1 is more resistant to block by tetrodotoxin (IC50 = 5.7 microM). hH1 is also resistant to mu-conotoxin but sensitive to block by therapeutic concentrations of lidocaine in a use-dependent manner.
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988, Pubmed,
Xenbase
Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988,
Pubmed
,
Xenbase Bean,
Lidocaine block of cardiac sodium channels.
1983,
Pubmed Brown,
Voltage clamp and internal perfusion of single rat heart muscle cells.
1981,
Pubmed Cachelin,
Sodium channels in cultured cardiac cells.
1983,
Pubmed Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Cohen,
Tetrodotoxin block of sodium channels in rabbit Purkinje fibers. Interactions between toxin binding and channel gating.
1981,
Pubmed Cruz,
Conus geographus toxins that discriminate between neuronal and muscle sodium channels.
1985,
Pubmed George,
Assignment of a human skeletal muscle sodium channel alpha-subunit gene (SCN4A) to 17q23.1-25.3.
1991,
Pubmed Guy,
Pursuing the structure and function of voltage-gated channels.
1990,
Pubmed
,
Xenbase Haimovich,
Localization of sodium channel subtypes in adult rat skeletal muscle using channel-specific monoclonal antibodies.
1987,
Pubmed Harris,
Studies on tetrodotoxin resistant action potentials in denervated skeletal muscle.
1971,
Pubmed Kallen,
Primary structure and expression of a sodium channel characteristic of denervated and immature rat skeletal muscle.
1990,
Pubmed Krafte,
Expression of cardiac Na channels with appropriate physiological and pharmacological properties in Xenopus oocytes.
1991,
Pubmed
,
Xenbase Kunze,
Cardiac Na currents and the inactivating, reopening, and waiting properties of single cardiac Na channels.
1985,
Pubmed Litt,
Localization of a human brain sodium channel gene (SCN2A) to chromosome 2.
1989,
Pubmed Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase Noda,
A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II.
1989,
Pubmed
,
Xenbase Pappone,
Voltage-clamp experiments in normal and denervated mammalian skeletal muscle fibres.
1980,
Pubmed Rogart,
Molecular cloning of a putative tetrodotoxin-resistant rat heart Na+ channel isoform.
1989,
Pubmed Rogart,
High-STX-affinity vs. low-STX-affinity Na+ channel subtypes in nerve, heart, and skeletal muscle.
1986,
Pubmed Scheuer,
Functional properties of rat brain sodium channels expressed in a somatic cell line.
1990,
Pubmed Sutton,
Tetrodotoxin-sensitive voltage-dependent Na currents recorded from Xenopus oocytes injected with mammalian cardiac muscle RNA.
1988,
Pubmed
,
Xenbase Tomaselli,
Human cardiac sodium channels expressed in Xenopus oocytes.
1990,
Pubmed
,
Xenbase Trimmer,
Primary structure and functional expression of a mammalian skeletal muscle sodium channel.
1989,
Pubmed
,
Xenbase Trimmer,
Molecular diversity of voltage-sensitive Na channels.
1989,
Pubmed Weiss,
Functional differences between two classes of sodium channels in developing rat skeletal muscle.
1986,
Pubmed White,
SkM2, a Na+ channel cDNA clone from denervated skeletal muscle, encodes a tetrodotoxin-insensitive Na+ channel.
1991,
Pubmed
,
Xenbase Yamamoto,
Voltage-dependent calcium block of normal and tetramethrin-modified single sodium channels.
1984,
Pubmed
