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Bovine chromaffin granule membranes undergo Ca(2+)-regulated exocytosis in frog oocytes.
Scheuner D, Logsdon CD, Holz RW.
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We have devised a new method that permits the investigation of exogenous secretory vesicle function using frog oocytes and bovine chromaffin granules, the secretory vesicles from adrenal chromaffin cells. Highly purified chromaffin granule membranes were injected into Xenopus laevis oocytes. Exocytosis was detected by the appearance of dopamine-beta-hydroxylase of the chromaffin granule membrane in the oocyteplasma membrane. The appearance of dopamine-beta-hydroxylase on the oocyte surface was strongly Ca(2+)-dependent and was stimulated by coinjection of the chromaffin granule membranes with InsP3 or Ca2+/EGTA buffer (18 microM free Ca2+) or by incubation of the injected oocytes in medium containing the Ca2+ ionophore ionomycin. Similar experiments were performed with a subcellular fraction from cultured chromaffin cells enriched with [3H]norepinephrine-containing chromaffin granules. Because the release of [3H]norepinephrine was strongly correlated with the appearance of dopamine-beta-hydroxylase on the oocyte surface, it is likely that intact chromaffin granules and chromaffin granule membranes undergo exocytosis in the oocyte. Thus, the secretory vesicle membrane without normal vesicle contents is competent to undergo the sequence of events leading to exocytosis. Furthermore, the interchangeability of mammalian and amphibian components suggests substantial biochemical conservation of the regulated exocytotic pathway during the evolutionary progression from amphibians to mammals.
Breckenridge,
Final steps in exocytosis observed in a cell with giant secretory granules.
1987, Pubmed
Breckenridge,
Final steps in exocytosis observed in a cell with giant secretory granules.
1987,
Pubmed Brooks,
Catecholamine secretion by chemically skinned cultured chromaffin cells.
1983,
Pubmed Busa,
Activation of frog (Xenopus laevis) eggs by inositol trisphosphate. I. Characterization of Ca2+ release from intracellular stores.
1985,
Pubmed
,
Xenbase Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase Dunn,
Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells.
1983,
Pubmed Elinson,
Fertilization in amphibians: the ancestry of the block to polyspermy.
1986,
Pubmed Grey,
Formation and structure of the fertilization envelope in Xenopus laevis.
1974,
Pubmed
,
Xenbase Hollinger,
Calcium-induced dehiscence of cortical granules in Xenopus laevis oocytes.
1979,
Pubmed
,
Xenbase Holz,
The role of osmotic forces in exocytosis from adrenal chromaffin cells.
1986,
Pubmed Holz,
Effects of osmolality and ionic strength on secretion from adrenal chromaffin cells permeabilized with digitonin.
1986,
Pubmed Kilpatrick,
Stability of bovine adrenal medulla cells in culture.
1980,
Pubmed Kline,
Fertilization events induced by neurotransmitters after injection of mRNA in Xenopus eggs.
1988,
Pubmed
,
Xenbase Knight,
Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields.
1982,
Pubmed Kopell,
Osmotic pressures of solutions of ATP and catecholamines relating to storage in chromaffin granules.
1982,
Pubmed Kubota,
Free calcium wave upon activation in Xenopus eggs.
1987,
Pubmed
,
Xenbase Lew,
Quantitative analysis of the cytosolic free calcium dependency of exocytosis from three subcellular compartments in intact human neutrophils.
1986,
Pubmed Neher,
Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
1982,
Pubmed Njus,
Bioenergetics of secretory vesicles.
1986,
Pubmed Patzak,
Exocytotic exposure and retrieval of membrane antigens of chromaffin granules: quantitative evaluation of immunofluorescence on the surface of chromaffin cells.
1984,
Pubmed Phillips,
Visualization of the exocytosis/endocytosis secretory cycle in cultured adrenal chromaffin cells.
1983,
Pubmed Wilson,
A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
1989,
Pubmed Wilson,
Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromaffin cells.
1983,
Pubmed Winkler,
The composition of adrenal chromaffin granules: an assessment of controversial results.
1976,
Pubmed Winkler,
The molecular organization of adrenal chromaffin granules.
1980,
Pubmed Zimmerberg,
Simultaneous electrical and optical measurements show that membrane fusion precedes secretory granule swelling during exocytosis of beige mouse mast cells.
1987,
Pubmed
