Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Unique N-acetyl-2-aminofluorene (AAF) or 2-aminofluorene (AF) adducts were introduced into the Xenopus borealis somatic 5S RNA gene between the intragenic control region and the transcription termination site. The effects of these bulky adducts on transcription were studied in a cell-free extract derived from Xenopus laevis oocytes. AAF and AF adducts inhibit transcription only when they are on the template strand, whereas transcription passes through these adducts when they are placed on the nontemplate strand. In the presence of the AAF or AF adduct on the template strand, transcription usually terminates one nucleotide before the altered guanine residue. Premature termination at these bulky adducts does not block reinitiation of transcription, since several transcripts are produced per gene per hour on these damaged templates.
Armier,
N-acetoxy-N-2-acetylaminofluorene-induced damage on SV40 DNA: inhibition of DNA replication and visualization of DNA lesions.
1988, Pubmed
Armier,
N-acetoxy-N-2-acetylaminofluorene-induced damage on SV40 DNA: inhibition of DNA replication and visualization of DNA lesions.
1988,
Pubmed Axelrod,
Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3'-deoxyribonucleoside 5'-triphosphate chain terminators.
1985,
Pubmed Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase Bohr,
DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall.
1985,
Pubmed Broyde,
Conformation of 2-aminofluorene-modified DNA.
1983,
Pubmed Burnouf,
Single adduct mutagenesis: strong effect of the position of a single acetylaminofluorene adduct within a mutation hot spot.
1989,
Pubmed Evans,
Sensitivity of the conformation of deoxyguanosine to binding at the C-8 position by N-acetylated and unacetylated 2-aminofluorene.
1980,
Pubmed Fuchs,
Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene.
1981,
Pubmed Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase Gupta,
Mutagenesis by single site-specific arylamine-DNA adducts. Induction of mutations at multiple sites.
1989,
Pubmed Hansson,
Localization of DNA repair synthesis by human cell extracts to a short region at the site of a lesion.
1989,
Pubmed Heflich,
Metabolism of 2-acetylaminofluorene in the Chinese hamster ovary cell mutation assay.
1988,
Pubmed Kaplan,
Preferential inhibition of the synthesis of 45S ribosomal rna precursor by N-acetoxyacetylaminofluorene in rat liver epithelial cultures.
1976,
Pubmed Madhani,
Differential DNA repair in transcriptionally active and inactive proto-oncogenes: c-abl and c-mos.
1986,
Pubmed Matsumoto,
Repair of a synthetic abasic site in DNA in a Xenopus laevis oocyte extract.
1989,
Pubmed
,
Xenbase Matsumoto,
Repair of a synthetic abasic site involves concerted reactions of DNA synthesis followed by excision and ligation.
1991,
Pubmed
,
Xenbase McConkey,
TFIIIA binds with equal affinity to somatic and major oocyte 5S RNA genes.
1988,
Pubmed
,
Xenbase Mellon,
Induction of the Escherichia coli lactose operon selectively increases repair of its transcribed DNA strand.
1989,
Pubmed Mellon,
Selective removal of transcription-blocking DNA damage from the transcribed strand of the mammalian DHFR gene.
1987,
Pubmed Mellon,
Preferential DNA repair of an active gene in human cells.
1986,
Pubmed Michaels,
Evidence for in vitro translesion DNA synthesis past a site-specific aminofluorene adduct.
1987,
Pubmed Miller,
Carcinogenesis by chemicals: an overview--G. H. A. Clowes memorial lecture.
1970,
Pubmed Millette,
The effect of modification of T7 DNA by the carcinogen N-1-acetylaminofluorene: termination of transcription in vitro.
1975,
Pubmed Moore,
Effect of acetylated and deacetylated 2-aminofluorene adducts on in vitro DNA synthesis.
1982,
Pubmed Moore,
Termination of vitro DNA synthesis at AAF adducts in the DNA.
1980,
Pubmed Moriya,
Targeted mutations induced by a single acetylaminofluorene DNA adduct in mammalian cells and bacteria.
1988,
Pubmed Nelson,
Modification of ribonucleic acid by chemical carcinogens. IV. Circular dichroism and proton magnetic resonance studies of oligonucleotides modified with N-2-acetylaminofluorene.
1971,
Pubmed Norman,
NMR and computational characterization of the N-(deoxyguanosin-8-yl)aminofluorene adduct [(AF)G] opposite adenosine in DNA: (AF)G[syn].A[anti] pair formation and its pH dependence.
1989,
Pubmed Orfanoudakis,
Repair of acetyl-aminofluorene modified pBR322 DNA in Xenopus laevis oocytes and eggs; effect of diadenosine tetraphosphate.
1990,
Pubmed
,
Xenbase Sands,
The carboxyterminal zinc fingers of TFIIIA interact with the tip of helix V of 5S RNA in the 7S ribonucleoprotein particle.
1991,
Pubmed
,
Xenbase Seeberg,
Acetylaminofluorene bound to different guanines of the sequence -GGCGCC- is excised with different efficiencies by the UvrABC excision nuclease in a pattern not correlated to the potency of mutation induction.
1990,
Pubmed Selby,
Transcription preferentially inhibits nucleotide excision repair of the template DNA strand in vitro.
1990,
Pubmed Shibutani,
Isolation and characterization of oligodeoxynucleotides containing dG-N2-AAF and oxidation products of dG-C8-AF.
1991,
Pubmed Strauss,
Role of DNA polymerase 3'----5' exonuclease activity in the bypass of aminofluorene lesions in DNA.
1990,
Pubmed Takeshita,
Oligodeoxynucleotides containing synthetic abasic sites. Model substrates for DNA polymerases and apurinic/apyrimidinic endonucleases.
1987,
Pubmed Tang,
Quantification of aminofluorene adduct formation and repair in defined DNA sequences in mammalian cells using the UVRABC nuclease.
1989,
Pubmed Vieira,
Production of single-stranded plasmid DNA.
1987,
Pubmed Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase Xing,
A 3' exonuclease activity degrades the pseudogene 5S RNA transcript and processes the major oocyte 5S RNA transcript in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
