Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1991 Oct 01;1010:2805-11. doi: 10.1002/j.1460-2075.1991.tb07829.x.
Show Gene links
Show Anatomy links
Cloning, expression, pharmacology and regulation of a delayed rectifier K+ channel in mouse heart.
Honoré E, Attali B, Romey G, Heurteaux C, Ricard P, Lesage F, Lazdunski M, Barhanin J.
???displayArticle.abstract???
Neonatal mouse cardiac poly(A)+ mRNA microinjection into Xenopus oocytes directed the expression of a delayed rectifier K+ current. A cDNA encoding this channel, called mIsK, was cloned from a neonatal mouse heart cDNA library whose properties were studied after expression of the complementary RNA in Xenopus oocytes. Among the different known K+ channel blockers, only the class III antiarrhythmic clofilium inhibited mIsK in the 10-100 microM range. The channel was completely insensitive to other antiarrhythmics such as quinine, quinidine, sotalol or amiodarone. mIsK was enhanced by increasing intracellular Ca2+ and by microinjected Ca(2+)-calmodulin dependent protein kinase II. These stimulations were reversed by the calmodulin antagonist W7. Conversely, the phorbol ester PMA, the diacylglycerol analog OAG and microinjected purified protein kinase C inhibited mIsK. This inhibitory effect could be prevented by the protein kinase C inhibitor staurosporine. These results were consistent with the presence of consensus sequences for kinase II and kinase C in the mIsK structure. Cultured newborn mouse ventricular cardiac cells exhibited a delayed rectifier K+ current which had biophysical properties similar to those of cloned mIsK and which was inhibited by clofilium and protein kinase C activators. In situ hybridization experiments revealed that mIsK mRNA was homogeneously distributed in the cardiac tissue. Neonatal mouse heart expressed the most mIsK mRNA compared with various other rat and mouse tissues. Since this K+ channel generates a current which appears to be involved in the control of both the action potential duration and the beating rate, these results suggest an important role for the mIsK channel in cardiac cell physiology and cardiac pathology.
Arena,
Block of heart potassium channels by clofilium and its tertiary analogs: relationship between drug structure and type of channel blocked.
1988, Pubmed
Arena,
Block of heart potassium channels by clofilium and its tertiary analogs: relationship between drug structure and type of channel blocked.
1988,
Pubmed Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed Bernardi,
Molecular properties of potassium channels.
1989,
Pubmed Betz,
Homology and analogy in transmembrane channel design: lessons from synaptic membrane proteins.
1990,
Pubmed Brown,
Regulation of heartbeat by G protein-coupled ion channels.
1990,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Dascal,
Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Folander,
Cloning and expression of the delayed-rectifier IsK channel from neonatal rat heart and diethylstilbestrol-primed rat uterus.
1990,
Pubmed
,
Xenbase HALL,
Current-voltage relations of Purkinje fibres in sodium-deficient solutions.
1963,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Iwai,
Characterization of gene organization and generation of heterogeneous mRNA species of rat ISK protein.
1990,
Pubmed Jan,
How might the diversity of potassium channels be generated?
1990,
Pubmed
,
Xenbase Kameyama,
Intracellular Na+ activates a K+ channel in mammalian cardiac cells.
,
Pubmed Kemp,
Protein kinase recognition sequence motifs.
1990,
Pubmed Kim,
Potassium channels in cardiac cells activated by arachidonic acid and phospholipids.
1989,
Pubmed Kuret,
Purification and characterization of a Ca2+/calmodulin-dependent protein kinase from rat brain.
1984,
Pubmed McDonald,
The potassium current underlying delayed rectification in cat ventricular muscle.
1978,
Pubmed Mukherjee,
Differences in myocardial alpha- and beta-adrenergic receptor numbers in different species.
1983,
Pubmed Murai,
Molecular cloning and sequence analysis of human genomic DNA encoding a novel membrane protein which exhibits a slowly activating potassium channel activity.
1989,
Pubmed
,
Xenbase Noble,
Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres.
1969,
Pubmed Noble,
The surprising heart: a review of recent progress in cardiac electrophysiology.
1984,
Pubmed Noma,
ATP-regulated K+ channels in cardiac muscle.
,
Pubmed Osterrieder,
Injection of subunits of cyclic AMP-dependent protein kinase into cardiac myocytes modulates Ca2+ current.
1982,
Pubmed Pragnell,
Estrogen induction of a small, putative K+ channel mRNA in rat uterus.
1990,
Pubmed
,
Xenbase Reuter,
Ion channels in cardiac cell membranes.
1984,
Pubmed Roberds,
Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart.
1991,
Pubmed Romey,
Targets for calcium channel blockers in mammalian skeletal muscle and their respective functions in excitation-contraction coupling.
1988,
Pubmed Rudy,
Diversity and ubiquity of K channels.
1988,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sanguinetti,
Two components of cardiac delayed rectifier K+ current. Differential sensitivity to block by class III antiarrhythmic agents.
1990,
Pubmed Séquier,
Regional expression of a MCD-peptide and dendrotoxin I-sensitive voltage-dependent potassium channel in rat brain.
1990,
Pubmed Shubeita,
Endothelin induction of inositol phospholipid hydrolysis, sarcomere assembly, and cardiac gene expression in ventricular myocytes. A paracrine mechanism for myocardial cell hypertrophy.
1990,
Pubmed Siegelbaum,
Calcium-activated transient outward current in calf cardiac Purkinje fibres.
1980,
Pubmed Stevens,
Ion channels. Making a submicroscopic hole in one.
1991,
Pubmed Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase Takumi,
Cloning of a membrane protein that induces a slow voltage-gated potassium current.
1988,
Pubmed
,
Xenbase Tamkun,
Molecular cloning and characterization of two voltage-gated K+ channel cDNAs from human ventricle.
1991,
Pubmed Tseng-Crank,
Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library.
1990,
Pubmed
,
Xenbase Walsh,
Regulation of a heart potassium channel by protein kinase A and C.
1988,
Pubmed Walton,
A three-step purification procedure for protein kinase C: characterization of the purified enzyme.
1987,
Pubmed Woodgett,
Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements.
1986,
Pubmed
