Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A strict temporal order of maternal mRNA translation is essential for meiotic cell cycle progression in oocytes of the frog Xenopus laevis. The molecular mechanisms controlling the ordered pattern of mRNA translational activation have not been elucidated. We report a novel role for the neural stem cell regulatory protein, Musashi, in controlling the translational activation of the mRNA encoding the Mos proto-oncogene during meiotic cell cycle progression. We demonstrate that Musashi interacts specifically with the polyadenylation response element in the 3' untranslated region of the Mos mRNA and that this interaction is necessary for early Mos mRNA translational activation. A dominant inhibitory form of Musashi blocks maternal mRNA cytoplasmic polyadenylation and meiotic cell cycle progression. Our data suggest that Musashi is a target of the initiating progesterone signaling pathway and reveal that late cytoplasmic polyadenylation element-directed mRNA translation requires early, Musashi-dependent mRNA translation. These findings indicate that Musashi function is necessary to establish the temporal order of maternal mRNA translation during Xenopus meiotic cell cycle progression.
Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997, Pubmed,
Xenbase
Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase Battelli,
The RNA-binding protein Musashi-1 regulates neural development through the translational repression of p21WAF-1.
2006,
Pubmed Bernstein,
Analyzing mRNA-protein complexes using a yeast three-hybrid system.
2002,
Pubmed Charlesworth,
The temporal control of Wee1 mRNA translation during Xenopus oocyte maturation is regulated by cytoplasmic polyadenylation elements within the 3'-untranslated region.
2000,
Pubmed
,
Xenbase Charlesworth,
A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation.
2002,
Pubmed
,
Xenbase Charlesworth,
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes.
2004,
Pubmed
,
Xenbase Colegrove-Otero,
RNA-binding proteins in early development.
2005,
Pubmed Collier,
The DAZL family proteins are PABP-binding proteins that regulate translation in germ cells.
2005,
Pubmed
,
Xenbase Daar,
Inhibition of mos-induced oocyte maturation by protein kinase A.
1993,
Pubmed
,
Xenbase de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase de Moor,
Mechanisms of translational control by the 3' UTR in development and differentiation.
2005,
Pubmed Dupré,
Mos is not required for the initiation of meiotic maturation in Xenopus oocytes.
2002,
Pubmed
,
Xenbase Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Freeman,
Meiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.
1991,
Pubmed
,
Xenbase Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase Good,
The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells.
1998,
Pubmed Good,
Three new members of the RNP protein family in Xenopus.
1993,
Pubmed
,
Xenbase Gray,
Multiple portions of poly(A)-binding protein stimulate translation in vivo.
2000,
Pubmed
,
Xenbase Gross,
The critical role of the MAP kinase pathway in meiosis II in Xenopus oocytes is mediated by p90(Rsk).
2000,
Pubmed
,
Xenbase Hemmati,
Cancerous stem cells can arise from pediatric brain tumors.
2003,
Pubmed Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase Huchon,
The pure inhibitor of cAMP-dependent protein kinase initiates Xenopus laevis meiotic maturation. A 4-step scheme for meiotic maturation.
1981,
Pubmed
,
Xenbase Imai,
The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA.
2001,
Pubmed Kanemura,
Musashi1, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity.
2001,
Pubmed Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase Kuersten,
The power of the 3' UTR: translational control and development.
2003,
Pubmed Lovell,
Ectopic expression of Musashi-1 in Alzheimer disease and Pick disease.
2005,
Pubmed Machaca,
Induction of maturation-promoting factor during Xenopus oocyte maturation uncouples Ca(2+) store depletion from store-operated Ca(2+) entry.
2002,
Pubmed
,
Xenbase Matten,
Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.
1996,
Pubmed
,
Xenbase Mendez,
Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction.
2002,
Pubmed
,
Xenbase Mignone,
UTRdb and UTRsite: a collection of sequences and regulatory motifs of the untranslated regions of eukaryotic mRNAs.
2005,
Pubmed Minshall,
A conserved role of a DEAD box helicase in mRNA masking.
2001,
Pubmed
,
Xenbase Murakami,
Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos.
1998,
Pubmed
,
Xenbase Nakajo,
Absence of Wee1 ensures the meiotic cell cycle in Xenopus oocytes.
2000,
Pubmed
,
Xenbase Okabe,
Translational repression determines a neuronal potential in Drosophila asymmetric cell division.
2001,
Pubmed Okano,
Musashi: a translational regulator of cell fate.
2002,
Pubmed Okano,
Function of RNA-binding protein Musashi-1 in stem cells.
2005,
Pubmed Paris,
Maturation-specific polyadenylation: in vitro activation by p34cdc2 and phosphorylation of a 58-kD CPE-binding protein.
1991,
Pubmed
,
Xenbase Potten,
Identification of a putative intestinal stem cell and early lineage marker; musashi-1.
2003,
Pubmed Qian,
The polo-like kinase Plx1 is required for activation of the phosphatase Cdc25C and cyclin B-Cdc2 in Xenopus oocytes.
2001,
Pubmed
,
Xenbase Roy,
Mos proto-oncogene function during oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sengupta,
Identification of RNAs that bind to a specific protein using the yeast three-hybrid system.
1999,
Pubmed SenGupta,
A three-hybrid system to detect RNA-protein interactions in vivo.
1996,
Pubmed Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed Toda,
Expression of the neural RNA-binding protein Musashi1 in human gliomas.
2001,
Pubmed Wang,
Progesterone inhibits protein kinase A (PKA) in Xenopus oocytes: demonstration of endogenous PKA activities using an expressed substrate.
2004,
Pubmed
,
Xenbase Wilkie,
Regulation of mRNA translation by 5'- and 3'-UTR-binding factors.
2003,
Pubmed Yokota,
Identification of differentially expressed and developmentally regulated genes in medulloblastoma using suppression subtraction hybridization.
2004,
Pubmed Zhang,
Yeast three-hybrid system to detect and analyze interactions between RNA and protein.
1999,
Pubmed
