Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
When a red cell nuclear extract (RCE) from adult chickens was injected into Xenopus oocytes along with the chicken beta globin gene, transcript levels were dramatically reduced compared to injection of DNA alone. The inhibitory action of the RCE was not specific to the beta globin gene since the Herpes thymidine kinase and Xenopus 5S RNA gene transcript levels were similarly reduced. Transcriptional repression was observed even after passage of the RCE through oocytecytoplasm to the nucleus. The inhibitory activity binds to DNA cellulose, which suggests that the inhibitor either binds to DNA or associates with DNA-binding proteins. Nuclease digestion of the chromatin assembled on injected beta globin DNA revealed that inhibition was not associated with local changes in chromatin structure. Extracts from 9-d chicken embryonic erythroid cells, in which the endogenous beta globin gene is actively expressed, did not inhibit transcription. The inhibitory activity is, therefore, restricted to transcriptionally quiescent, adult erythrocytes. Since the inhibitory effects were seen with both polymerase II and III directed genes, we speculate that the activity may be part of the extreme transcriptional repression which occurs in the terminally differentiated erythrocyte.
Bendig,
Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.
1984, Pubmed,
Xenbase
Bendig,
Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.
1984,
Pubmed
,
Xenbase CAMERON,
RNA and protein metabolism in the maturation of the nucleated chicken erythrocyte.
1963,
Pubmed DAVIES,
Structure in nucleated erythrocytes.
1961,
Pubmed Dingwall,
Protein import into the cell nucleus.
1986,
Pubmed Emerson,
Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
1984,
Pubmed Emerson,
Erythroid-specific activation and derepression of the chick beta-globin promoter in vitro.
1989,
Pubmed Emerson,
Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain.
1985,
Pubmed Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed Gallarda,
The beta-globin stage selector element factor is erythroid-specific promoter/enhancer binding protein NF-E4.
1989,
Pubmed Gargiulo,
Analysis of the chromatin assembled in germinal vesicles of Xenopus oocytes.
1983,
Pubmed
,
Xenbase Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase Jackson,
Developmental modulation of protein binding to beta-globin gene regulatory sites within chicken erythrocyte nuclei.
1989,
Pubmed Jones,
Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase Kernell,
Chromatin changes during erythropoiesis.
1971,
Pubmed Landes,
Hemoglobin switching in chickens. Is the switch initiated post-transcriptionally?
1982,
Pubmed Lewis,
An erythrocyte-specific protein that binds to the poly(dG) region of the chicken beta-globin gene promoter.
1988,
Pubmed Maclean,
The RNA of chicken erythrocytes.
1973,
Pubmed Madgwick,
RNA synthesis in chicken erythrocytes.
1972,
Pubmed McGhee,
A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
1981,
Pubmed McKnight,
A colorimetric method for the determination of submicrogram quantities of protein.
1977,
Pubmed Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
1982,
Pubmed
,
Xenbase Mohun,
Upstream sequences required for tissue-specific activation of the cardiac actin gene in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase Old,
Activity of a cloned Xenopus albumin gene promoter in the homologous frog oocyte system.
1988,
Pubmed
,
Xenbase Partington,
Human globin gene transcription in injected Xenopus oocytes: enhancement by sodium butyrate.
1984,
Pubmed
,
Xenbase Plumb,
Multiple sequence-specific DNA binding activities are eluted from chicken nuclei at low ionic strengths.
1985,
Pubmed Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Ruiz-Carrillo,
Changes in histone acetyl content and in nuclear non-histone protein composition of avian erythroid cells at different stages of maturation.
1974,
Pubmed Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase Schorpp,
5'-flanking and 5'-proximal exon regions of the two Xenopus albumin genes. Deletion analysis of constitutive promoter function.
1988,
Pubmed
,
Xenbase Walmsley,
Highly efficient beta globin transcription in the absence of both a viral enhancer and erythroid factors.
1987,
Pubmed
,
Xenbase Weintraub,
Assembly and propagation of repressed and depressed chromosomal states.
1985,
Pubmed Zentgraf,
Characterization and localization of the RNA synthesized in mature avian erythrocytes.
1975,
Pubmed
