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Gamma-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in mammalian brain, is known to interact with two classes of GABA receptors denoted GABAA and GABAB. Using Xenopus oocytes, we compared the electrical and pharmacological properties of GABA receptors expressed by poly(A)+ RNA isolated from mammalian brain and retina. RNA from cerebral cortex expressed GABA responses with features characteristic of currents mediated by GABAA receptors. In contrast, RNA from retina expressed responses mediated by GABAA receptors and, in addition, GABA responses that were insensitive to the GABAA antagonist bicuculline and the GABAB agonist baclofen and showed no modulation by barbiturates or benzodiazepines. The bicuculline/baclofen-insensitive GABA response was a Cl- current that was blocked by picrotoxin but showed little desensitization or outward rectification. Our results suggest that mammalian retina contains RNAs encoding GABA receptors with distinct pharmacology.
Aoshima,
Minimal model to account for the membrane conductance increase and desensitization of gamma-aminobutyric acid receptors synthesized in the Xenopus oocytes injected with rat brain mRNA.
1987, Pubmed,
Xenbase
Aoshima,
Minimal model to account for the membrane conductance increase and desensitization of gamma-aminobutyric acid receptors synthesized in the Xenopus oocytes injected with rat brain mRNA.
1987,
Pubmed
,
Xenbase Barnard,
Functional expression in the Xenopus oocyte of messenger ribonucleic acids encoding brain neurotransmitter receptors: further characterisation of the implanted GABA receptor.
1987,
Pubmed
,
Xenbase Blair,
Single subunits of the GABAA receptor form ion channels with properties of the native receptor.
1988,
Pubmed
,
Xenbase Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Curtis,
Bicuculline, an antagonist of GABA and synaptic inhibition in the spinal cord of the cat.
1971,
Pubmed Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase Hill,
3H-baclofen and 3H-GABA bind to bicuculline-insensitive GABA B sites in rat brain.
1981,
Pubmed Houamed,
Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA.
,
Pubmed
,
Xenbase Kerr,
2-Hydroxy-saclofen: an improved antagonist at central and peripheral GABAB receptors.
1988,
Pubmed Krogsgaard-Larsen,
Inhibitors of the GABA uptake systems.
1980,
Pubmed Larsson,
Uptake of GABA and nipecotic acid in astrocytes and neurons in primary cultures: changes in the sodium coupling ratio during differentiation.
1986,
Pubmed Levitan,
Structural and functional basis for GABAA receptor heterogeneity.
1988,
Pubmed
,
Xenbase Levitan,
Biophysical and pharmacological properties of cloned GABAA receptor subunits expressed in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Luthe,
A simple technique for the preparation and storage of sucrose gradients.
1983,
Pubmed Massey,
Transmitter circuits in the vertebrate retina.
1987,
Pubmed Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase Miledi,
Effects of defolliculation on membrane current responses of Xenopus oocytes.
1989,
Pubmed
,
Xenbase Miledi,
Synthesis of chick brain GABA receptors by frog oocytes.
1982,
Pubmed
,
Xenbase Miledi,
Chloride current induced by injection of calcium into Xenopus oocytes.
1984,
Pubmed
,
Xenbase Olsen,
Drug interactions at the GABA receptor-ionophore complex.
1982,
Pubmed Oron,
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
,
Pubmed
,
Xenbase Parker,
Changes in intracellular calcium and in membrane currents evoked by injection of inositol trisphosphate into Xenopus oocytes.
1986,
Pubmed
,
Xenbase Parker,
Responses to GABA, glycine and beta-alanine induced in Xenopus oocytes by messenger RNA from chick and rat brain.
1988,
Pubmed
,
Xenbase Parker,
Messenger RNA from bovine retina induces kainate and glycine receptors in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Parker,
Actions of pentobarbital on rat brain receptors expressed in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Schofield,
Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family.
,
Pubmed
,
Xenbase Shivers,
Two novel GABAA receptor subunits exist in distinct neuronal subpopulations.
1989,
Pubmed Sigel,
Allosteric modulation by benzodiazepine receptor ligands of the GABAA receptor channel expressed in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sigel,
The effect of subunit composition of rat brain GABAA receptors on channel function.
1990,
Pubmed
,
Xenbase Smart,
mRNA-directed synthesis and insertion of functional amino acid receptors in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase Verdoorn,
Functional properties of recombinant rat GABAA receptors depend upon subunit composition.
1990,
Pubmed Ymer,
GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.
1989,
Pubmed
,
Xenbase Ymer,
Sequence and expression of a novel GABAA receptor alpha subunit.
1989,
Pubmed
,
Xenbase
