Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1991 May 01;115:2517-28. doi: 10.1128/mcb.11.5.2517-2528.1991.
Show Gene links
Show Anatomy links
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
Posada J, Sanghera J, Pelech S, Aebersold R, Cooper JA.
???displayArticle.abstract???
Meiotic maturation of Xenopus and sea star oocytes involves the activation of a number of protein-serine/threonine kinase activities, including a myelin basic protein (MBP) kinase. A 44-kDa MBP kinase (p44mpk) purified from mature sea star oocytes is shown here to be phosphorylated at tyrosine. Antiserum to purified sea star p44mpk was used to identify antigenically related proteins in Xenopus oocytes. Two tyrosine-phosphorylated 42-kDa proteins (p42) were detected with this antiserum in Xenopus eggs. Xenopus p42 chromatographs with MBP kinase activity on a Mono Q ion-exchange column. Tyrosine phosphorylation of Xenopus p42 approximately parallels MBP kinase activity during meiotic maturation. These results suggest that related MBP kinases are activated during meiotic maturation of Xenopus and sea star oocytes. Previous studies have suggested that Xenopus p42 is related to the mitogen-activated protein (MAP) kinases of culture mammalian cells. We have cloned a MAP kinase relative from a Xenopus ovary cDNA library and demonstrate that this clone encodes the Xenopus p42 that is tyrosine phosphorylated during oocyte maturation. Comparison of the sequences of Xenopus p42 and a rat MAP kinase (ERK1) and peptide sequences from sea star p44mpk indicates that these proteins are close relatives. The family members appear to be tyrosine phosphorylated, and activated, in different contexts, with the murine MAP kinase active during the transition from quiescence to the G1 stage of the mitotic cell cycle and the sea star and Xenopus kinases being active during M phase of the meiotic cell cycle.
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987, Pubmed
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987,
Pubmed Ahn,
Evidence for an epidermal growth factor-stimulated protein kinase cascade in Swiss 3T3 cells. Activation of serine peptide kinase activity by myelin basic protein kinases in vitro.
1990,
Pubmed Ahn,
Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.
1990,
Pubmed Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase Boulton,
Evidence for insulin-dependent activation of S6 and microtubule-associated protein-2 kinases via a human insulin receptor/v-ros hybrid.
1990,
Pubmed Boulton,
An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control.
1990,
Pubmed Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Chen,
Identification of Xenopus S6 protein kinase homologs (pp90rsk) in somatic cells: phosphorylation and activation during initiation of cell proliferation.
1990,
Pubmed
,
Xenbase Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Cooper,
Major substrate for growth factor-activated protein-tyrosine kinases is a low-abundance protein.
1985,
Pubmed Cooper,
Similar effects of platelet-derived growth factor and epidermal growth factor on the phosphorylation of tyrosine in cellular proteins.
1982,
Pubmed Cooper,
Related proteins are phosphorylated at tyrosine in response to mitogenic stimuli and at meiosis.
1989,
Pubmed
,
Xenbase Cotten,
Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs.
1986,
Pubmed
,
Xenbase Courchesne,
A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae.
1989,
Pubmed Dorée,
Activity of the maturation-promoting factor and the extent of protein phosphorylation oscillate simultaneously during meiotic maturation of starfish oocytes.
1983,
Pubmed Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase Ely,
A 42-kD tyrosine kinase substrate linked to chromaffin cell secretion exhibits an associated MAP kinase activity and is highly related to a 42-kD mitogen-stimulated protein in fibroblasts.
1990,
Pubmed Erikson,
Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
1987,
Pubmed
,
Xenbase Erikson,
In vivo phosphorylation and activation of ribosomal protein S6 kinases during Xenopus oocyte maturation.
1989,
Pubmed
,
Xenbase Ferrell,
Identification of a 42-kilodalton phosphotyrosyl protein as a serine(threonine) protein kinase by renaturation.
1990,
Pubmed Garrels,
Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines.
1979,
Pubmed Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase Gould,
Protein kinase C phosphorylates pp60src at a novel site.
1985,
Pubmed Hanekom,
Complexing of the CD-3 subunit by a monoclonal antibody activates a microtubule-associated protein 2 (MAP-2) serine kinase in Jurkat cells.
1989,
Pubmed Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed Hanocq-Quertier,
Phosphorylation of ribosomal proteins during maturation of Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase Haystead,
Okadaic acid mimics the action of insulin in stimulating protein kinase activity in isolated adipocytes. The role of protein phosphatase 2a in attenuation of the signal.
1990,
Pubmed Hoshi,
Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.
1988,
Pubmed Hoshi,
Characterization of a mitogen-activated, Ca2+-sensitive microtubule-associated protein-2 kinase.
1989,
Pubmed Howell,
STY, a tyrosine-phosphorylating enzyme with sequence homology to serine/threonine kinases.
1991,
Pubmed Kalthoff,
Ribosomal protein S6 from Xenopus laevis ovaries. Isolation, phosphorylation in vivo and cross-reaction with heterologous anti-S6 antibodies.
1982,
Pubmed
,
Xenbase Karsenti,
Phosphorylation changes associated with the early cell cycle in Xenopus eggs.
1987,
Pubmed
,
Xenbase Kazlauskas,
Protein kinase C mediates platelet-derived growth factor-induced tyrosine phosphorylation of p42.
1988,
Pubmed Kohno,
Diverse mitogenic agents induce rapid phosphorylation of a common set of cellular proteins at tyrosine in quiescent mammalian cells.
1985,
Pubmed Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase Lohka,
Metaphase protein phosphorylation in Xenopus laevis eggs.
1987,
Pubmed
,
Xenbase Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase Martin-Pérez,
Activation of ribosomal protein S6 phosphorylation during meiotic maturation of Xenopus laevis oocytes: in vitro ordered appearance of S6 phosphopeptides.
1986,
Pubmed
,
Xenbase Miyasaka,
Nerve growth factor stimulates a protein kinase in PC-12 cells that phosphorylates microtubule-associated protein-2.
1990,
Pubmed Morrison,
Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF beta-receptor.
1989,
Pubmed Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase Pelech,
Protein kinase cascades in meiotic and mitotic cell cycle control.
1990,
Pubmed
,
Xenbase Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed Pelech,
Characterization of maturation-activated histone H1 and ribosomal S6 kinases in sea star oocytes.
1987,
Pubmed Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed Ray,
Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
1988,
Pubmed Ray,
Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.
1988,
Pubmed Ray,
Insulin-stimulated microtubule associated protein kinase is detectable by analytical gel chromatography as a 35-kDa protein in myocytes, adipocytes, and hepatocytes.
1988,
Pubmed Rossomando,
Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase.
1989,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sanghera,
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
1990,
Pubmed Saxton,
Tubulin dynamics in cultured mammalian cells.
1984,
Pubmed Steele,
The two Xenopus laevis SRC genes are co-expressed and each produces functional pp60src.
1989,
Pubmed
,
Xenbase Stefanovic,
Post-transcriptional regulation by insulin of Xenopus ribosomal protein S6 kinase.
1988,
Pubmed
,
Xenbase Stith,
Induction of meiotic maturation in Xenopus oocytes by 12-O-tetradecanoylphorbol 13-acetate.
1987,
Pubmed
,
Xenbase Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase Sweet,
Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.
1990,
Pubmed
,
Xenbase Vila,
Mitogen-stimulated tyrosine phosphorylation of a 42-kD cellular protein: evidence for a protein kinase-C requirement.
1988,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
