Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The stability and integrity of mRNAs encoding neurotransmitter receptors and voltage-activated channels in the postmortem rat brain was investigated by isolating poly(A)+ mRNA, injecting it into Xenopus oocytes, and then examining the expression of functional neurotransmitter receptors and voltage-activated channels in the oocyte membrane by electrophysiological recording. This approach was also used to assess the stability of mRNAs in brains that were incubated in oxygenated mammalian Ringer's solution for various lengths of time and from brains that were freshly frozen and then thawed at room temperature. Oocytes injected with mRNA from up to 21-hr postmortem brains gave large agonist- and voltage-activated responses, indicating that mRNAs encoding neurotransmitter receptors and voltage-activated channels are relatively stable in postmortem brain tissue. In contrast, oocytes injected with mRNA from brains incubated in Ringer's solution exhibited smaller responses, and oocytes injected with mRNA from tissue that was frozen and then thawed displayed very small or undetectable responses. Northern blot analysis using a nucleic acid probe for rat brain Na(+)-channel mRNA indicated that the size of the Na+ currents in injected oocytes reflected the levels of mRNA for Na+ channels in the different mRNA preparations. Thus, the expressional potency of mRNAs encoding neurotransmitter receptors and voltage-activated channels is quite stable in postmortem brains in situ, but it is reduced if the brains are kept in oxygenated saline, and freezing and thawing of tissue results in rapid degeneration of mRNA.
Akagi,
Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.
1989, Pubmed,
Xenbase
Akagi,
Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.
1989,
Pubmed
,
Xenbase Akagi,
Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord.
1988,
Pubmed
,
Xenbase Alberghina,
Age-related changes of ribonuclease activities in various regions of the rat central nervous system.
1988,
Pubmed Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase Carpenter,
Expression of GABA and glycine receptors by messenger RNAs from the developing rat cerebral cortex.
1988,
Pubmed
,
Xenbase Franzoni,
Rat brain ribonucleases.
1978,
Pubmed Gilbert,
The preparation of biologically active messenger RNA from human postmortem brain tissue.
1981,
Pubmed Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase Gundersen,
Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Gundersen,
Voltage-operated channels induced by foreign messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Gundersen,
Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes.
1984,
Pubmed
,
Xenbase Houamed,
Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA.
,
Pubmed
,
Xenbase Johnson,
Extensive postmortem stability of RNA from rat and human brain.
1986,
Pubmed Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
,
Xenbase Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase McGann,
Manifestations of cell damage after freezing and thawing.
1988,
Pubmed Miledi,
Chloride current induced by injection of calcium into Xenopus oocytes.
1984,
Pubmed
,
Xenbase Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase Morrison,
The isolation and in vitro translation of undegraded messenger RNAs from human postmortem brain.
1981,
Pubmed Noda,
Existence of distinct sodium channel messenger RNAs in rat brain.
,
Pubmed Parker,
Neurotensin and substance P receptors expressed in Xenopus oocytes by messenger RNA from rat brain.
1986,
Pubmed
,
Xenbase Reichert,
In vitro study of the biological activity of RNAs after incubation of hog liver, heart and brain tissue at room temperature.
1985,
Pubmed Rowe,
Cryopreservation of granulocytes for transfusion: studies on human granulocyte isolation, the effect of glycerol on lysosomes, kinetics of glycerol uptake and cryopreservation with dimethyl sulfoxide and glycerol.
1980,
Pubmed Sajdel-Sulkowska,
In vitro synthesis of polypeptides of moderately large size by poly(A)-containing messenger RNA from postmortem human brain and mouse brain.
1983,
Pubmed Sajdel-Sulkowska,
Alzheimer's disease brain: alterations in RNA levels and in a ribonuclease-inhibitor complex.
1984,
Pubmed Schulz-Harder,
Comparison of brain ribonucleases of rabbit, guinea pig, rat, mouse and gerbil.
1988,
Pubmed Sigel,
Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid.
1987,
Pubmed
,
Xenbase Sumikawa,
Messenger RNA from rat brain induces noradrenaline and dopamine receptors in Xenopus oocytes.
1984,
Pubmed
,
Xenbase Sumikawa,
Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.
1984,
Pubmed
,
Xenbase
