Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We report the isolation and sequence of a cDNA clone that encodes a locust (Schistocerca gregaria) nervous system nicotinic acetylcholine receptor (AChR) subunit (alpha L1). The calculated molecular weight of the unglycosylated polypeptide, which contains in the proposed extracellular domain two adjacent cysteine residues which are characteristic of alpha (ligand binding) subunits, is 60,641 daltons. Injection into Xenopus oocytes, of RNA synthesized from this clone in vitro, results in expression of functional nicotinic receptors in the oocyte membrane. In these, nicotine opens a cation channel; the receptors are blocked by both alpha-bungarotoxin (alpha-Bgt) and kappa-bungarotoxin (kappa-Bgt). Reversible block of the expressed insect AChR by mecamylamine, d-tubocurarine, tetraethylammonium, bicuculline and strychnine has also been observed. These data are entirely consistent with previously reported electrophysiological studies on in vivo insect nicotinic receptors and also with biochemical studies on an alpha-Bgt affinity purified locust AChR. Thus, a functional receptor exhibiting the characteristic pharmacology of an in vivo insect nicotinic AChR can be expressed in Xenopus oocytes by injection with a single subunit RNA.
Barton,
A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
1987, Pubmed
Barton,
A strategy for the rapid multiple alignment of protein sequences. Confidence levels from tertiary structure comparisons.
1987,
Pubmed Bertrand,
Activation and blocking of neuronal nicotinic acetylcholine receptor reconstituted in Xenopus oocytes.
1990,
Pubmed
,
Xenbase Blagburn,
Internal presynaptic tetraethylammonium (TEA+) blocks cholinergic transmission at a synapse between identified neurones.
1987,
Pubmed Blair,
Single subunits of the GABAA receptor form ion channels with properties of the native receptor.
1988,
Pubmed
,
Xenbase Bossy,
Conservation of neural nicotinic acetylcholine receptors from Drosophila to vertebrate central nervous systems.
1988,
Pubmed Boulter,
Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family.
1987,
Pubmed
,
Xenbase Boulter,
Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor alpha-subunit.
,
Pubmed Boulter,
Isolation of a clone coding for the alpha-subunit of a mouse acetylcholine receptor.
1985,
Pubmed Breer,
Molecular forms and subunit structure of the acetylcholine receptor in the central nervous system of insects.
1985,
Pubmed Buonanno,
A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit.
1986,
Pubmed Chiappinelli,
Kappa-bungarotoxin blocks nicotinic transmission at an identified invertebrate central synapse.
1989,
Pubmed de la Garza,
Selective antagonism of nicotine actions in the rat cerebellum with alpha-bungarotoxin.
1987,
Pubmed Deneris,
Primary structure and expression of beta 2: a novel subunit of neuronal nicotinic acetylcholine receptors.
1988,
Pubmed
,
Xenbase Deneris,
Beta 3: a new member of nicotinic acetylcholine receptor gene family is expressed in brain.
1989,
Pubmed Drummond,
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Duvoisin,
The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: beta 4.
1989,
Pubmed
,
Xenbase Filbin,
Partial purification and characterisation of an acetylcholine receptor with nicotinic properties from the supraoesophageal ganglion of the locust (Schistocerca gregaria).
1983,
Pubmed Finer-Moore,
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.
1984,
Pubmed Freeman,
Effect of alpha-bungarotoxin on retinotectal synaptic transmission in the goldfish and the toad.
1980,
Pubmed Goldman,
Members of a nicotinic acetylcholine receptor gene family are expressed in different regions of the mammalian central nervous system.
1987,
Pubmed Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed Hanke,
Channel properties of an insect neuronal acetylcholine receptor protein reconstituted in planar lipid bilayers.
,
Pubmed Hermans-Borgmeyer,
Primary structure of a developmentally regulated nicotinic acetylcholine receptor protein from Drosophila.
1986,
Pubmed Kao,
Identification of the alpha subunit half-cystine specifically labeled by an affinity reagent for the acetylcholine receptor binding site.
1984,
Pubmed Kao,
Acetylcholine receptor binding site contains a disulfide cross-link between adjacent half-cystinyl residues.
1986,
Pubmed Lane,
Differential accessibility to two insect neurones does not account for differences in sensitivity to alpha-bungarotoxin.
1982,
Pubmed Lindstrom,
Molecular studies of the neuronal nicotinic acetylcholine receptor family.
1987,
Pubmed Loring,
Characterization of neuronal nicotinic receptors by snake venom neurotoxins.
1988,
Pubmed Marshall,
Cloning of putative nicotinic acetylcholine receptor genes from the locust.
1988,
Pubmed Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed Nef,
Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors.
1988,
Pubmed Neumann,
Mapping of the alpha-bungarotoxin binding site within the alpha subunit of the acetylcholine receptor.
1986,
Pubmed Noda,
Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.
1983,
Pubmed Parker,
A slowly inactivating potassium current in native oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase Pinnock,
Kappa-bungarotoxin blocks an alpha-bungarotoxin-sensitive nicotinic receptor in the insect central nervous system.
1988,
Pubmed Pritchett,
Transient expression shows ligand gating and allosteric potentiation of GABAA receptor subunits.
1988,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Sattelle,
GABA receptors on the cell-body membrane of an identified insect motor neuron.
1988,
Pubmed Schloss,
Neuronal acetylcholine receptors in Drosophila: the ARD protein is a component of a high-affinity alpha-bungarotoxin binding complex.
1988,
Pubmed Schmieden,
Functional expression in Xenopus oocytes of the strychnine binding 48 kd subunit of the glycine receptor.
1989,
Pubmed
,
Xenbase Snyder,
Neurotransmitter receptors in the brain: biochemical identification.
1976,
Pubmed Sontheimer,
Functional chloride channels by mammalian cell expression of rat glycine receptor subunit.
1989,
Pubmed von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed Wada,
Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor.
1988,
Pubmed
,
Xenbase Wafford,
gamma-Aminobutyric acid-activated 36Cl- influx: a functional in vitro assay for CNS gamma-aminobutyric acid receptors of insects.
1987,
Pubmed White,
Mouse-Torpedo hybrid acetylcholine receptors: functional homology does not equal sequence homology.
1985,
Pubmed
,
Xenbase
