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Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocytenucleus: a generalized, facilitated process.
Bataillé N, Helser T, Fried HM.
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To study the biochemistry of ribonucleoprotein export from the nucleus, we characterized an in vivo assay in which the cytoplasmic appearance of radiolabeled ribosomal subunits was monitored after their microinjection into Xenopus oocyte nuclei. Denaturing gel electrophoresis and sucrose density gradient sedimentation demonstrated that injected subunits were transported intact. Consistent with the usual subcellular distribution of ribosomes, transport was unidirectional, as subunits injected into the cytoplasm did not enter the nucleus. Transport displayed properties characteristic of a facilitated, energy-dependent process; the rate of export was saturable and transport was completely inhibited either by lowering the temperature or by depleting nuclei of ATP; the effect of lowered temperature was completely reversible. Transport of injected subunits was likely a process associated with the nuclear pore complex, since export was also inhibited by prior or simultaneous injection of wheat germ agglutinin, a lectin known to inhibit active nuclear transport by binding to N-acetyl glucosamine-containing glycoproteins present in the NPC (Hart, G. W., R. S. Haltiwanger, G. D. Holt, and W. G. Kelly. 1989. Annu. Rev. Biochem. 58:841-874). Although GlcNAc modified proteins exist on both the nuclear and cytoplasmic sides of the nuclear pore complex, ribosomal subunit export was inhibited only when wheat germ agglutinin was injected into the nucleus. Finally, we found that ribosomal subunits from yeast and Escherichia coli were efficiently exported from Xenopus oocyte nuclei, suggesting that export of some RNP complexes may be directed by a collective biochemical property rather than by specific macromolecular primary sequences or structures.
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989, Pubmed
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989,
Pubmed Agutter,
Nucleocytoplasmic RNA transport.
1984,
Pubmed Akey,
Protein import through the nuclear pore complex is a multistep process.
1989,
Pubmed
,
Xenbase Amaldi,
Expression of ribosomal protein genes and regulation of ribosome biosynthesis in Xenopus development.
1989,
Pubmed
,
Xenbase Anderson,
Synthesis of heterogeneous nuclear RNA in full-grown oocytes of Xenopus laevis (Daudin).
1977,
Pubmed
,
Xenbase Baglia,
Nuclear ribonucleoprotein release and nucleoside triphosphatase activity are inhibited by antibodies directed against one nuclear matrix glycoprotein.
1983,
Pubmed Bernd,
Modulation of the nuclear-envelope nucleoside triphosphatase by poly(A)-rich mRNA and by microtubule protein.
1982,
Pubmed Bogdanov,
The role of ribonucleic acids in the organization and functioning of ribosomes of E. coli.
1980,
Pubmed Borer,
Major nucleolar proteins shuttle between nucleus and cytoplasm.
1989,
Pubmed
,
Xenbase Breeuwer,
Facilitated nuclear transport of histone H1 and other small nucleophilic proteins.
1990,
Pubmed Cande,
Nucleotide requirements for anaphase chromosome movements in permeabilized mitotic cells: anaphase B but not anaphase A requires ATP.
1982,
Pubmed Clawson,
Nucleocytoplasmic RNA transport.
1985,
Pubmed Clawson,
A model for nucleocytoplasmic transport of ribonucleoprotein particles.
1982,
Pubmed Dabauvalle,
Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin.
1988,
Pubmed
,
Xenbase Davis,
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.
1987,
Pubmed Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Dreyfuss,
Heterogeneous nuclear ribonucleoprotein particles and the pathway of mRNA formation.
1988,
Pubmed Dworetzky,
Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
1988,
Pubmed
,
Xenbase Featherstone,
A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
1988,
Pubmed
,
Xenbase Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase Feldherr,
Manual enucleation of Xenopus oocytes.
1978,
Pubmed
,
Xenbase FELDHERR,
THE EFFECT OF THE ELECTRON-OPAQUE PORE MATERIAL ON EXCHANGES THROUGH THE NUCLEAR ANNULI.
1965,
Pubmed Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase Flynn,
Peptide binding and release by proteins implicated as catalysts of protein assembly.
1989,
Pubmed Goldfarb,
Nuclear transport.
1989,
Pubmed Guddat,
Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes.
1990,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Hanover,
O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins.
1987,
Pubmed Holt,
Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.
1987,
Pubmed Hügle,
Ribocharin: a nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit.
1985,
Pubmed
,
Xenbase Jiang,
Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
1986,
Pubmed Khanna-Gupta,
Nucleocytoplasmic transport of ribosomes in a eukaryotic system: is there a facilitated transport process?
1989,
Pubmed
,
Xenbase Legrain,
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
1989,
Pubmed Li,
Identification of a human protein that interacts with nuclear localization signals.
1989,
Pubmed Mehlin,
Structure and transport of a specific premessenger RNP particle.
1988,
Pubmed Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase Paine,
Nuclear envelope permeability.
1975,
Pubmed Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase Richardson,
Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
1988,
Pubmed
,
Xenbase Rubin,
The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.
1973,
Pubmed Schröder,
Studies on protein kinases involved in regulation of nucleocytoplasmic mRNA transport.
1988,
Pubmed Silver,
Yeast proteins that recognize nuclear localization sequences.
1989,
Pubmed
,
Xenbase Snow,
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.
1987,
Pubmed
,
Xenbase Steitz,
A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells.
1988,
Pubmed Stevens,
RNA transport from nucleus to cytoplasm in Chironomus salivary glands.
1966,
Pubmed Sugino,
Energy coupling in DNA gyrase and the mechanism of action of novobiocin.
1978,
Pubmed Tobian,
tRNA nuclear transport: defining the critical regions of human tRNAimet by point mutagenesis.
1985,
Pubmed
,
Xenbase Underwood,
Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.
1990,
Pubmed Warner,
Synthesis of ribosomes in Saccharomyces cerevisiae.
1989,
Pubmed Wolff,
Nuclear protein import: specificity for transport across the nuclear pore.
1988,
Pubmed Yamasaki,
Identification of four nuclear transport signal-binding proteins that interact with diverse transport signals.
1989,
Pubmed
,
Xenbase Yoneda,
Reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells.
1987,
Pubmed
,
Xenbase Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
