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Chromosoma
1990 Oct 01;996:379-90. doi: 10.1007/bf01726689.
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Characterization of a second highly conserved B-type lamin present in cells previously thought to contain only a single B-type lamin.
Höger TH, Zatloukal K, Waizenegger I, Krohne G.
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Previous analyses of the nuclear lamina of mammalian cells have revealed three major protein components (lamins A, B and C) that have been identified by protein sequence homology as members of the intermediate filament (IF) protein family. It has been claimed that mammalian cells contain either all three lamins or lamin B alone. Using monoclonal antibodies specific for B-type lamins and cDNA cloning we identified a second major mammalian B-type lamin (murine lamin B2), thus showing that lamin composition in mammals is more complex than previously thought. Lamin B2 is coexpressed with lamin B1 (formerly termed lamin B) in all somatic cells and mammalian species that we analysed, including a variety of cells currently believed to contain only a single lamin. This suggests that two B-type lamins are necessary to form a functional lamina in mammalian somatic cells. By cDNA cloning we found that Xenopus laevis lamin LII is the amphibian homolog of mammalian lamin B2. Lamin expression during embryogenesis of amphibians and mammals shows striking similarities. The first lamins expressed in the early embryo are the two B-type lamins, while A-type lamins are only detected much later in development. These findings indicate that the genomic differentiation into two B-type lamins occurred early in vertebrate evolution and has been maintained in both their primary structure and pattern of expression.
Aebi,
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, Pubmed,
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Aebi,
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,
Pubmed
,
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Incorporation of a product of mevalonic acid metabolism into proteins of Chinese hamster ovary cell nuclei.
1988,
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Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.
1985,
Pubmed
,
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Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
1985,
Pubmed
,
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Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies.
1986,
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1986,
Pubmed Chelsky,
Lamin B methylation and assembly into the nuclear envelope.
1989,
Pubmed Chelsky,
Cell cycle-dependent methyl esterification of lamin B.
1987,
Pubmed Cowin,
Plakoglobin: a protein common to different kinds of intercellular adhering junctions.
1986,
Pubmed Doetschman,
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1985,
Pubmed Farnsworth,
Identification of geranylgeranyl-modified proteins in HeLa cells.
1990,
Pubmed Feinberg,
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1983,
Pubmed Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed Franke,
Nuclear lamins and cytoplasmic intermediate filament proteins: a growing multigene family.
1987,
Pubmed Georgatos,
Lamin B constitutes an intermediate filament attachment site at the nuclear envelope.
1987,
Pubmed Gerace,
Organization and modulation of nuclear lamina structure.
1984,
Pubmed Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed Guilly,
A human T lymphoblastic cell line lacks lamins A and C.
1987,
Pubmed Hazan,
Change of cytokeratin organization during development of Mallory bodies as revealed by a monoclonal antibody.
1986,
Pubmed Höger,
Characterization of a second highly conserved B-type lamin present in cells previously thought to contain only a single B-type lamin.
1991,
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Amino acid sequence and molecular characterization of murine lamin B as deduced from cDNA clones.
1988,
Pubmed
,
Xenbase Holtz,
The CaaX motif of lamin A functions in conjunction with the nuclear localization signal to target assembly to the nuclear envelope.
1989,
Pubmed Ierardi,
Synaptonemal complexes are integral components of the isolated mouse spermatocyte nuclear matrix.
1983,
Pubmed Jackson,
Formation of cytoskeletal elements during mouse embryogenesis. Intermediate filaments of the cytokeratin type and desmosomes in preimplantation embryos.
1980,
Pubmed Jahn,
Cytokeratins in certain endothelial and smooth muscle cells of two taxonomically distant vertebrate species, Xenopus laevis and man.
1987,
Pubmed
,
Xenbase Jorcano,
Identification of two types of keratin polypeptides within the acidic cytokeratin subfamily I.
1984,
Pubmed Kartenbeck,
Attachment of vimentin filaments to desmosomal plaques in human meningiomal cells and arachnoidal tissue.
1984,
Pubmed Kaufmann,
Additional members of the rat liver lamin polypeptide family. Structural and immunological characterization.
1989,
Pubmed Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase Krohne,
Cell type-specific differences in protein composition of nuclear pore complex-lamina structures in oocytes and erythrocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase Krohne,
The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.
1989,
Pubmed
,
Xenbase Krohne,
A monoclonal antibody against nuclear lamina proteins reveals cell type-specificity in Xenopus laevis.
1984,
Pubmed
,
Xenbase Krohne,
Proteins of pore complex--lamina structures from nuclei and nuclear membranes.
1983,
Pubmed
,
Xenbase Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase Krohne,
Nuclear lamin LI of Xenopus laevis: cDNA cloning, amino acid sequence and binding specificity of a member of the lamin B subfamily.
1987,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lebel,
Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells.
1987,
Pubmed Lehner,
Differential expression of nuclear lamin proteins during chicken development.
1987,
Pubmed
,
Xenbase Lehner,
The nuclear lamin protein family in higher vertebrates. Identification of quantitatively minor lamin proteins by monoclonal antibodies.
1986,
Pubmed Longo,
Basic proteins of the perinuclear theca of mammalian spermatozoa and spermatids: a novel class of cytoskeletal elements.
1987,
Pubmed Maul,
Identification and redistribution of lamins during nuclear differentiation in mouse spermatogenesis.
1986,
Pubmed Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
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Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
,
Pubmed Nigg,
The nuclear envelope.
1989,
Pubmed O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed Peter,
Cloning and sequencing of cDNA clones encoding chicken lamins A and B1 and comparison of the primary structures of vertebrate A- and B-type lamins.
1989,
Pubmed Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed Riedel,
Nucleotide sequence of the full-length mouse lamin C cDNA and its deduced amino-acid sequence.
1989,
Pubmed Rilling,
Prenylated proteins: the structure of the isoprenoid group.
1990,
Pubmed Röber,
Differential timing of nuclear lamin A/C expression in the various organs of the mouse embryo and the young animal: a developmental study.
1989,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schöler,
New type of POU domain in germ line-specific protein Oct-4.
1990,
Pubmed Senior,
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.
1988,
Pubmed Stewart,
Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B.
1987,
Pubmed Stick,
The fates of chicken nuclear lamin proteins during mitosis: evidence for a reversible redistribution of lamin B2 between inner nuclear membrane and elements of the endoplasmic reticulum.
1988,
Pubmed Stick,
cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis.
1988,
Pubmed
,
Xenbase Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase Stick,
The disappearance of the nuclear lamina during spermatogenesis: an electron microscopic and immunofluorescence study.
1982,
Pubmed Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed Vorburger,
Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif.
1989,
Pubmed Vorburger,
A second higher vertebrate B-type lamin. cDNA sequence determination and in vitro processing of chicken lamin B2.
1989,
Pubmed Weber,
Protein chemical analysis of purified murine lamin B identifies two distinct polypeptides B1 and B2.
1990,
Pubmed Weber,
Maturation of nuclear lamin A involves a specific carboxy-terminal trimming, which removes the polyisoprenylation site from the precursor; implications for the structure of the nuclear lamina.
1989,
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Evidence for modification of lamin B by a product of mevalonic acid.
1988,
Pubmed Wolin,
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1987,
Pubmed
,
Xenbase Worman,
A lamin B receptor in the nuclear envelope.
1988,
Pubmed Worman,
Nuclear lamina heterogeneity in mammalian cells. Differential expression of the major lamins and variations in lamin B phosphorylation.
1988,
Pubmed
