Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Expression of rat liver Na+/L-alanine co-transport in Xenopus laevis oocytes. Effect of glucagon in vivo.
Palacin M, Werner A, Dittmer J, Murer H, Biber J.
???displayArticle.abstract???
Poly(A)+ RNA (mRNA) isolated from rat liver was injected into Xenopus laevis oocytes, and expression of Na+/L-alanine transport was assayed by measuring Na(+)-dependent uptake of L-[3H]alanine. Expression of Na+/L-alanine transport was detected 3-7 days after mRNA injection, and was due to an increment of the Na(+)-dependent component. After injection of 40 ng of total mRNA, Na(+)-dependent uptake of L-alanine was 2.5-fold higher than in water-injected oocytes. In contrast with Na+/L-alanine transport by water-injected oocytes, expressed Na+/L-alanine transport was inhibited by N-methylaminoisobutyric acid, was inhibited by an extracellular pH of 6.5 and was saturated at approx. 1 mM-L-alanine. After sucrose-density-gradient fractionation, highest expression of Na+/L-alanine uptake was observed with mRNA of 1.9-2.5 kb in length. Compared with mRNA isolated from control rats, mRNA isolated from glucagon-treated rats showed a approx. 2-fold higher expression of Na+/L-alanine transport. The results demonstrate that both liver Na+/L-alanine transport systems (A and ASC) can be expressed in X. laevis oocytes. Furthermore, the data obtained with mRNA isolated from glucagon-treated rats suggest that glucagon regulates liver Na+/L-alanine transport (at least in part) via the availability of the corresponding mRNA.
Aoshima,
Expression of amino acid transport systems in Xenopus oocytes injected with mRNA of rat small intestine and kidney.
1988, Pubmed,
Xenbase
Aoshima,
Expression of amino acid transport systems in Xenopus oocytes injected with mRNA of rat small intestine and kidney.
1988,
Pubmed
,
Xenbase Barber,
Induction of amino acid transport system A in rat hepatocytes is blocked by tunicamycin.
1983,
Pubmed Bracy,
Solubilization and reconstitution of hepatic System A-mediated amino acid transport. Preparation of proteoliposomes containing glucagon-stimulated transport activity.
1987,
Pubmed Campa,
Characterization of neutral and cationic amino acid transport in Xenopus oocytes.
1989,
Pubmed
,
Xenbase Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Christensen,
Organic ion transport during seven decades. The amino acids.
1984,
Pubmed Collarini,
Mechanisms of transport of amino acids across membranes.
1987,
Pubmed Edmondson,
Comparative studies of alanine and alpha-aminoisobutyric acid uptake by freshly isolated rat liver cells.
1979,
Pubmed Edmondson,
Direct measurement of active transport systems for alanine in freshly isolated rat liver cells.
1977,
Pubmed Edmondson,
Biphasic stimulation of amino acid uptake by glucagon in hepatocytes.
1980,
Pubmed Fafournoux,
Solubilization and reconstitution characteristics of hepatic system A-mediated amino acid transport.
1989,
Pubmed Fehlmann,
Insulin and glucagon stimulation of amino acid transport in isolated rat hepatocytes. Synthesis of a high affinity component of transport.
1979,
Pubmed Goldin,
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Handlogten,
Induction and decay of amino acid transport in the liver. Turnover of transport activity in isolated hepatocytes after stimulation by diabetes or glucagon.
1984,
Pubmed Hayes,
Comparison of the effects of certain thiol reagents on alanine transport in plasma membrane vesicles from rat liver and their use in identifying the alanine carrier.
1983,
Pubmed Hayes,
Differential effects of starvation on alanine and glutamine transport in isolated rat hepatocytes.
1982,
Pubmed Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase Jung,
Sodium-alanine cotransport in oocytes of Xenopus laevis: correlation of alanine and sodium fluxes with potential and current changes.
1984,
Pubmed
,
Xenbase Kilberg,
Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine, and closely related analogs.
1980,
Pubmed Kilberg,
Characteristics of system ASC for transport of neutral amino acids in the isolated rat hepatocyte.
1981,
Pubmed Kilberg,
Amino acid transport in isolated rat hepatocytes.
1982,
Pubmed Kilberg,
Cysteine as a system-specific substrate for transport system ASC in rat hepatocytes.
1979,
Pubmed Le Cam,
Neutral amino acid transport. Characterization of the A and L systems in isolated rat hepatocytes.
1977,
Pubmed McCormick,
Effect of alkali cations on freeze-thaw-dependent reconstitution of amino acid transport from Ehrlich ascites cell plasma membrane.
1985,
Pubmed McCormick,
Simple and effective purification of a Na+-dependent amino acid transport system from Ehrlich ascites cell plasma membrane.
1988,
Pubmed McGivan,
Stimulation of alanine transport and metabolism by dibutyryl cyclic AMP in the hepatocytes from fed rats. Assessment of transport as a potential rate-limiting step for alanine metabolism.
1981,
Pubmed Pariza,
Induction and decay of glucagon-induced amino acid transport in primary cultures of adult rat liver cells: paradoxical effects of cycloheximide and puromycin.
1976,
Pubmed Quesada,
A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A.
1988,
Pubmed Rosenthal,
Diabetes enhances activity of alanine transport in liver plasma membrane vesicles.
1985,
Pubmed Saier,
Neutral amino acid transport systems in animal cells: potential targets of oncogene action and regulators of cellular growth.
1988,
Pubmed Schenerman,
Maintenance of glucagon-stimulated system A amino acid transport activity in rat liver plasma membrane vesicles.
1986,
Pubmed Shotwell,
The regulation of neutral amino acid transport in mammalian cells.
1983,
Pubmed Sips,
Amino acid transport in plasma-membrane vesicles from rat liver. Characterization of L-alanine transport.
1980,
Pubmed Van Winkle,
Amino acid transport in developing animal oocytes and early conceptuses.
1988,
Pubmed
