Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Effects of the v-mos oncogene on Xenopus development: meiotic induction in oocytes and mitotic arrest in cleaving embryos.
Freeman RS, Kanki JP, Ballantyne SM, Pickham KM, Donoghue DJ.
???displayArticle.abstract???
Previous work has demonstrated that the Xenopus protooncogene mosxe can induce the maturation of prophase-arrested Xenopus oocytes. Recently, we showed that mosxe can transform murine NIH3T3 fibroblasts, although it exhibited only 1-2% of the transforming activity of the v-mos oncogene. In this study we have investigated the ability of the v-mos protein to substitute for the mosxe protein in stimulating Xenopus oocytes to complete meiosis. Microinjection of in vitro synthesized RNAs encoding either the mosxe or v-mos proteins stimulates resting oocytes to undergo germinal vesicle breakdown. Microinjection of an antisense oligonucleotide spanning the initiation codon of the mosxe gene blocked progesterone-induced oocyte maturation. When oocytes were microinjected first with the mosxe antisense oligonucleotide, and subsequently with in vitro synthesized v-mos RNA, meiotic maturation was rescued as evidenced by germinal vesicle breakdown. The v-mos protein exhibited in vitro kinase activity when recovered by immunoprecipitation from either microinjected Xenopus oocytes or transfected monkey COS-1 cells; however, in parallel experiments, we were unable to detect in vitro kinase activity associated with the mosxe protein. Microinjection of in vitro synthesized v-mos RNA into cleaving Xenopus embryos resulted in mitotic arrest, demonstrating that the v-mos protein can function like the mosxe protein as a component of cytostatic factor. These results exemplify the apparently conflicting effects of the v-mos protein, namely, its ability to induce maturation of oocytes, its ability to arrest mitotic cleavage of Xenopus embryo, and its ability to transform mammalian fibroblasts.
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988, Pubmed,
Xenbase
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase Audet,
Eukaryotic initiation factor 4A stimulates translation in microinjected Xenopus oocytes.
1987,
Pubmed
,
Xenbase Barrett,
Ha-rasVal-12,Thr-59 activates S6 kinase and p34cdc2 kinase in Xenopus oocytes: evidence for c-mosxe-dependent and -independent pathways.
1990,
Pubmed
,
Xenbase Blair,
Activation of the transforming potential of a normal cell sequence: a molecular model for oncogenesis.
1981,
Pubmed Blair,
Analysis of the transforming potential of the human homolog of mos.
1986,
Pubmed Booher,
Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control.
1989,
Pubmed Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
,
Xenbase Deshpande,
Insulin induction of Xenopus laevis oocyte maturation is inhibited by monoclonal antibody against p21 ras proteins.
1987,
Pubmed
,
Xenbase Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase Dunphy,
Unraveling of mitotic control mechanisms.
1988,
Pubmed
,
Xenbase Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed Freeman,
Transforming mutant v-mos protein kinases that are deficient in in vitro autophosphorylation.
1989,
Pubmed Freeman,
Xenopus homolog of the mos protooncogene transforms mammalian fibroblasts and induces maturation of Xenopus oocytes.
1989,
Pubmed
,
Xenbase Gallick,
Recognition of mos-related proteins with an antiserum to a peptide of the v-mos gene product.
1985,
Pubmed Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase Hannink,
Lysine residue 121 in the proposed ATP-binding site of the v-mos protein is required for transformation.
1985,
Pubmed Hannink,
Deletions in the C-terminal coding region of the v-sis gene: dimerization is required for transformation.
1986,
Pubmed Korn,
Ras p21 as a potential mediator of insulin action in Xenopus oocytes.
1987,
Pubmed
,
Xenbase Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
,
Xenbase Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed Laskey,
Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
1977,
Pubmed
,
Xenbase Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Maxwell,
Use of site-specific antipeptide antibodies to perturb the serine kinase catalytic activity of p37mos.
1985,
Pubmed Maxwell,
Serine kinase activity associated with Maloney murine sarcoma virus-124-encoded p37mos.
1985,
Pubmed Meijer,
Cyclin is a component of the sea urchin egg M-phase specific histone H1 kinase.
1989,
Pubmed Melton,
Translation of messenger RNA in injected frog oocytes.
1987,
Pubmed
,
Xenbase Merriam,
Progesterone-induced maturational events in oocytes of Xenopus laevis. II. Change in intracellular calcium and magnesium distribution at germinal vesicle breakdown.
1971,
Pubmed
,
Xenbase Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase Ohkura,
The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.
1989,
Pubmed O'Keefe,
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
1989,
Pubmed Papkoff,
Detection of a transforming gene product in cells transformed by Moloney murine sarcoma virus.
1982,
Pubmed Papkoff,
The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein.
1983,
Pubmed Paules,
Mouse Mos protooncogene product is present and functions during oogenesis.
1989,
Pubmed
,
Xenbase Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed Propst,
Proto-oncogene expression in germ cell development.
1988,
Pubmed Russell,
Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog.
1987,
Pubmed Russell,
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.
1987,
Pubmed Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Schmidt,
Chicken homolog of the mos proto-oncogene.
1988,
Pubmed Singh,
p37mos-associated serine/threonine protein kinase activity correlates with the cellular transformation function of v-mos.
1986,
Pubmed Singh,
Moloney murine sarcoma virus encoded p37mos expressed in yeast has protein kinase activity.
1986,
Pubmed Sprague,
Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein.
1983,
Pubmed Van Beveren,
Nucleotide sequence of the genome of a murine sarcoma virus.
1981,
Pubmed van der Hoorn,
Molecularly cloned c-mos(rat) is biologically active.
1982,
Pubmed Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase
