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Eleven distinct VH gene families and additional patterns of sequence variation suggest a high degree of immunoglobulin gene complexity in a lower vertebrate, Xenopus laevis.
Haire RN, Amemiya CT, Suzuki D, Litman GW.
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Lower vertebrate species, including Xenopus laevis, exhibit restricted antibody diversity relative to higher vertebrates. We have analyzed more than 180 VH gene-containing recombinant clones from an unamplified spleen cDNA library by selective sequencing of JH and CH positive clones following iterative hybridization screening with family-specific VH probes, 11 unique families of VH genes, each associated with a unique genomic Southern blot hybridization pattern, are described and compared. Considerable variation in the number of hybridizing components detected by each probe is evident. The nucleotide sequence difference between VH families is as great as, if not more than, that reported in other systems, including representatives of the mammalian, avian, and elasmobranch lineages. Some Xenopus Ig gene families encode alternative amino acids at positions that are otherwise invariant or very rarely substituted in known Igs. Furthermore, variations in complementarity determining region sequences among members of the same gene family and high degrees of DH and JH region complexity are described, suggesting that in at least this lower vertebrate species, the diversity of expressed Ig VH genes is not restricted.
Alt,
Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.
1982,
Pubmed Alt,
Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
1982,
Pubmed Amemiya,
Complete nucleotide sequence of an immunoglobulin heavy-chain gene and analysis of immunoglobulin gene organization in a primitive teleost species.
1990,
Pubmed Amemiya,
Nucleotide sequence of a cDNA encoding a third distinct Xenopus immunoglobulin heavy chain isotype.
1989,
Pubmed
,
Xenbase Berman,
Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.
1988,
Pubmed Brandt,
Antibody diversity in amphibians: evidence for the inheritance of idiotypic specificities in isogenic Xenopus.
1980,
Pubmed
,
Xenbase Brodeur,
The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes.
1984,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Clem,
Phylogeny of immunoglobulin structure and function. IV. Immunoglobulins of the giant grouper, Epinephelus itaira.
1971,
Pubmed Cohen,
Conservation and divergence of immunoglobulin VH pseudogenes.
1983,
Pubmed Cohen,
Simple DNA sequences in homologous flanking regions near immunoglobulin VH genes: a role in gene interaction?
1982,
Pubmed Crews,
A single VH gene segment encodes the immune response to phosphorylcholine: somatic mutation is correlated with the class of the antibody.
1981,
Pubmed Deverson,
Monoclonal BALB/c anti-progesterone antibodies use family IX variable region heavy chain genes.
1987,
Pubmed Du Pasquier,
Antibody diversity in amphibians: inheritance of isoelectric focusing antibody patterns in isogenic frogs.
1978,
Pubmed
,
Xenbase Du Pasquier,
The immune system of Xenopus.
1989,
Pubmed
,
Xenbase Du Pasquier,
Antibody diversity in lower vertebrates--why is it so restricted?
1982,
Pubmed Early,
An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
1980,
Pubmed Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Haire,
A second Xenopus immunoglobulin heavy chain constant region isotype gene.
1989,
Pubmed
,
Xenbase Harding,
Immunoglobulin heavy chain gene organization and complexity in the skate, Raja erinacea.
1990,
Pubmed Hartman,
VH genes encoding the immune response to beta-(1,6)-galactan: somatic mutation in IgM molecules.
1984,
Pubmed Hinds,
Major reorganization of immunoglobulin VH segmental elements during vertebrate evolution.
,
Pubmed Hsu,
Evolution of immunoglobulin genes: VH families in the amphibian Xenopus.
1989,
Pubmed
,
Xenbase Hsu,
A third immunoglobulin class in amphibians.
1985,
Pubmed
,
Xenbase Hsu,
Studies on Xenopus immunoglobulins using monoclonal antibodies.
1984,
Pubmed
,
Xenbase Kokubu,
Diverse organization of immunoglobulin VH gene loci in a primitive vertebrate.
1988,
Pubmed Kokubu,
Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate.
1988,
Pubmed Lawler,
Adult B-cell repertoire is biased toward two heavy-chain variable-region genes that rearrange frequently in fetal pre-B cells.
1987,
Pubmed Litman,
The range and fine specificity of the anti-hapten immune response: phylogenetic studies.
1982,
Pubmed Litman,
Complete nucleotide sequences of three VH genes in Caiman, a phylogenetically ancient reptile: evolutionary diversification in coding segments and variation in the structure and organization of recombination elements.
1985,
Pubmed Litman,
Immunoglobulin VH gene structure and diversity in Heterodontus, a phylogenetically primitive shark.
1985,
Pubmed Mäkelä,
Lack of heterogeneity in antihapten antibodies of a phylogenetically primitive shark.
1980,
Pubmed Max,
Variation in the crossover point of kappa immunoglobulin gene V-J recombination: evidence from a cryptic gene.
1980,
Pubmed Near,
Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains.
1984,
Pubmed Ollo,
Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
1981,
Pubmed Parvari,
Chicken immunoglobulin gamma-heavy chains: limited VH gene repertoire, combinatorial diversification by D gene segments and evolution of the heavy chain locus.
1988,
Pubmed Rathbun,
Molecular characterization of the A/J J558 family of heavy chain variable region gene segments.
1988,
Pubmed Reininger,
Novel V genes encode virtually identical variable regions of six murine monoclonal anti-bromelain-treated red blood cell autoantibodies.
1987,
Pubmed Reynaud,
A hyperconversion mechanism generates the chicken light chain preimmune repertoire.
1987,
Pubmed Reynaud,
A single rearrangement event generates most of the chicken immunoglobulin light chain diversity.
1985,
Pubmed Saiki,
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
1988,
Pubmed Sakano,
Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
1980,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schiff,
The Ig germline gene repertoire: economy or wastage?
1988,
Pubmed Schroeder,
Structure and evolution of mammalian VH families.
1990,
Pubmed Schwager,
Amino acid sequence of heavy chain from Xenopus laevis IgM deduced from cDNA sequence: implications for evolution of immunoglobulin domains.
1988,
Pubmed
,
Xenbase Schwager,
Genetic basis of the antibody repertoire in Xenopus: analysis of the Vh diversity.
1989,
Pubmed
,
Xenbase Schwager,
Organization and rearrangement of immunoglobulin M genes in the amphibian Xenopus.
1988,
Pubmed
,
Xenbase Shamblott,
Genomic organization and sequences of immunoglobulin light chain genes in a primitive vertebrate suggest coevolution of immunoglobulin gene organization.
1989,
Pubmed Shankey,
Phylogeny of immunoglobulin structure and function. IX. Intramolecular heterogeneity of shark 19S IgM antibodies to the dinitrophenyl hapten.
1980,
Pubmed Shlomchik,
Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). II. Comparison of hybridomas derived by lipopolysaccharide stimulation and secondary protein immunization.
1987,
Pubmed Wabl,
Antibody patterns in genetically identical frogs.
1976,
Pubmed
,
Xenbase Winter,
Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes.
1985,
Pubmed Yamawaki-Kataoka,
Nucleotide sequences of variable region segments of the immunoglobulin heavy chain of Xenopus laevis.
1987,
Pubmed
,
Xenbase Yancopoulos,
Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines.
,
Pubmed
