Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Rat brain expresses a heterogeneous family of calcium channels.
Snutch TP, Leonard JP, Gilbert MM, Lester HA, Davidson N.
???displayArticle.abstract???
We describe the isolation and characterization of several rat brain cDNAs that are homologous to the alpha 1 subunit of heart and skeletal muscle dihydropyridine-sensitive Ca channels. Northern blot analysis of 32 cDNAs shows that they can be grouped into four distinct classes (A, B, C, and D), each corresponding to a distinct hybridization pattern of brain mRNAs. Southern blot and DNA sequencing suggest that each class of cDNA represents a distinct gene or gene family. In the regions sequenced, the rat brain class C and D gene products share approximately 75% amino acid identity with the rabbit skeletal muscle Ca channel. In addition, the class C polypeptide is almost identical to the rabbit cardiac Ca channel (97% identity). In contrast, the rat brain class A and B cDNAs are more distantly related to dihydropyridine-sensitive Ca channels (47-64% amino acid identity) and to the brain class C and D genes (51-55% amino acid identity). To examine the functional significance of the isolated brain cDNAs, hybrid depletion experiments were performed in Xenopus oocytes. Antisense oligonucleotides against class A and B cDNAs each partially inhibited, and a class C oligonucleotide almost fully inhibited, the expression of Ba current in rat brain mRNA injected oocytes; but none of the oligonucleotides affected the expression of voltage-gated Na or K conductances. The clone characterization and sequencing results demonstrate that a number of distinct, yet related, voltage-gated Ca-channel genes are expressed in the brain. The antisense oligonucleotide experiments specifically show that one or several of the Ca-channel classes are related to the Ca channels observed in rat brain mRNA injected oocytes.
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980, Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988,
Pubmed
,
Xenbase Biggin,
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
1983,
Pubmed Campbell,
The biochemistry and molecular biology of the dihydropyridine-sensitive calcium channel.
1988,
Pubmed Catterall,
Molecular properties of dihydropyridine-sensitive calcium channels in skeletal muscle.
1988,
Pubmed Dash,
Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
1987,
Pubmed
,
Xenbase Ellis,
Sequence and expression of mRNAs encoding the alpha 1 and alpha 2 subunits of a DHP-sensitive calcium channel.
1988,
Pubmed Fedulova,
Two types of calcium channels in the somatic membrane of new-born rat dorsal root ganglion neurones.
1985,
Pubmed Fox,
Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurones.
1987,
Pubmed Kawasaki,
Quantitative hybridization-arrest of mRNA in Xenopus oocytes using single-stranded complementary DNA or oligonucleotide probes.
1985,
Pubmed
,
Xenbase Kayano,
Primary structure of rat brain sodium channel III deduced from the cDNA sequence.
1988,
Pubmed Leonard,
Ca channels induced in Xenopus oocytes by rat brain mRNA.
1987,
Pubmed
,
Xenbase Llinás,
Blocking and isolation of a calcium channel from neurons in mammals and cephalopods utilizing a toxin fraction (FTX) from funnel-web spider poison.
1989,
Pubmed Mikami,
Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.
1989,
Pubmed
,
Xenbase Noda,
Existence of distinct sodium channel messenger RNAs in rat brain.
,
Pubmed Nowycky,
Three types of neuronal calcium channel with different calcium agonist sensitivity.
,
Pubmed Perez-Reyes,
Induction of calcium currents by the expression of the alpha 1-subunit of the dihydropyridine receptor from skeletal muscle.
1989,
Pubmed Reuter,
Calcium channel modulation by neurotransmitters, enzymes and drugs.
,
Pubmed Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed Ruth,
Primary structure of the beta subunit of the DHP-sensitive calcium channel from skeletal muscle.
1989,
Pubmed Snutch,
The use of Xenopus oocytes to probe synaptic communication.
1988,
Pubmed
,
Xenbase Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase Tanabe,
Restoration of excitation-contraction coupling and slow calcium current in dysgenic muscle by dihydropyridine receptor complementary DNA.
1988,
Pubmed Tanabe,
Primary structure of the receptor for calcium channel blockers from skeletal muscle.
,
Pubmed Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed Tsien,
Calcium channels in excitable cell membranes.
1983,
Pubmed
