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Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.
Reichelt R, Holzenburg A, Buhle EL, Jarnik M, Engel A, Aebi U.
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Nuclear pore complexes (NPCs) prepared from Xenopus laevis oocyte nuclear envelopes were studied in "intact" form (i.e., unexposed to detergent) and after detergent treatment by a combination of conventional transmission electron microscopy (CTEM) and quantitative scanning transmission electron microscopy (STEM). In correlation-averaged CTEM pictures of negatively stained intact NPCs and of distinct NPC components (i.e., "rings," "spoke" complexes, and "plug-spoke" complexes), several fine structural features arranged with octagonal symmetry about a central axis could reproducibly be identified. STEM micrographs of unstained/freeze-dried intact NPCs as well as of their components yielded comparable but less distinct features. Mass determination by STEM revealed the following molecular masses: intact NPC with plug, 124 +/- 11 MD; intact NPC without plug, 112 +/- 11 MD; heavy ring, 32 +/- 5 MD; light ring, 21 +/- 4 MD; plug-spoke complex, 66 +/- 8 MD; and spoke complex, 52 +/- 3 MD. Based on these combined CTEM and STEM data, a three-dimensional model of the NPC exhibiting eightfold centrosymmetry about an axis perpendicular to the plane of the nuclear envelope but asymmetric along this axis is proposed. This structural polarity of the NPC across the nuclear envelope is in accord with its well-documented functional polarity facilitating mediated nucleocytoplasmic exchange of molecules and particles.
Abelson,
Nuclear pores: the pore-annulus relationship in thin section.
1970, Pubmed
Abelson,
Nuclear pores: the pore-annulus relationship in thin section.
1970,
Pubmed Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase Akey,
Protein import through the nuclear pore complex is a multistep process.
1989,
Pubmed
,
Xenbase Akey,
Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy.
1989,
Pubmed
,
Xenbase Blobel,
Gene gating: a hypothesis.
1985,
Pubmed Bonner,
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
1975,
Pubmed
,
Xenbase Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase Buhle,
The structure of the Ca2+ ATPase as revealed by electron microscopy and image processing of ordered arrays.
1983,
Pubmed Buhle,
Correlation of surface topography of metal-shadowed specimens with their negatively stained reconstructions.
1985,
Pubmed
,
Xenbase De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase Dingwall,
Protein import into the cell nucleus.
1986,
Pubmed Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Dworetzky,
Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
1988,
Pubmed
,
Xenbase Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed Engel,
Mass mapping of a protein complex with the scanning transmission electron microscope.
1982,
Pubmed Engel,
Processing of quantitative scanning transmission electron micrographs.
1988,
Pubmed Engel,
Molecular weight determination by scanning transmission electron microscopy.
1978,
Pubmed Engel,
Preparation of unstained protein structures for mass determination by electron scattering.
1980,
Pubmed Feldherr,
The uptake of endogenous proteins by oocyte nuclei.
1975,
Pubmed
,
Xenbase Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase Franke,
Structure, biochemistry, and functions of the nuclear envelope.
1974,
Pubmed Franke,
The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. I. The mature oocyte.
1970,
Pubmed Franke,
The nuclear envelope and the architecture of the nuclear periphery.
1981,
Pubmed Gall,
Octagonal nuclear pores.
1967,
Pubmed Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed Harris,
Some electron microscopic studies on intact nuclear 'ghosts' and nuclear membrane fragments.
1974,
Pubmed Harris,
The biochemistry and ultrastructure of the nuclear envelope.
1978,
Pubmed Hoeijmakers,
Structure of the nuclear pore complex in mammalian cells. Two annular components.
1974,
Pubmed Kartenbeck,
The nuclear envelope in freeze-etching.
1971,
Pubmed
,
Xenbase Krohne,
The major polypeptides of the nuclear pore complex.
1978,
Pubmed
,
Xenbase Leapman,
Quantitative electron energy loss spectroscopy in biology.
1988,
Pubmed Maul,
The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
1977,
Pubmed Newport,
The nucleus: structure, function, and dynamics.
1987,
Pubmed Paine,
Nuclear envelope permeability.
1975,
Pubmed Unwin,
A large particle associated with the perimeter of the nuclear pore complex.
1982,
Pubmed
,
Xenbase Unwin,
Three-dimensional model of membrane-bound ribosomes obtained by electron microscopy.
1977,
Pubmed Wrigley,
The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy.
1968,
Pubmed Zimmer,
The function of the nuclear envelope in nuclear protein accumulation.
1988,
Pubmed
,
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