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Cloning and expression of the delayed-rectifier IsK channel from neonatal rat heart and diethylstilbestrol-primed rat uterus.
Folander K, Smith JS, Antanavage J, Bennett C, Stein RB, Swanson R.
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cDNAs encoding a delayed-rectifier-type K+ channel were cloned from both neonatal rat heart and ovariectomized, diethylstilbestrol-primed rat uterus by using the polymerase chain reaction. Both clones have nucleotide sequences identical to that encoding the rat kidneyIsK channel [Takumi, T., Ohkubo, H. & Nakanishi, S. (1988) Science 242, 1042-1045] and encode a putative protein of 130 amino acids. Injection of RNA transcripts of the cDNAs into Xenopus oocytes resulted in the expression of a slowly activating, voltage-dependent K+ current. An antisense oligonucleotide, derived from the sequence of the clone, specifically inhibited the expression of the slow, outward current observed in cells injected with mRNAs isolated from the parent tissues (i.e., kidney, heart, and uterus), indicating that the cloned gene underlies the major K+ current expressed from RNA isolated from these tissues.
Arena,
Block of heart potassium channels by clofilium and its tertiary analogs: relationship between drug structure and type of channel blocked.
1988, Pubmed
Arena,
Block of heart potassium channels by clofilium and its tertiary analogs: relationship between drug structure and type of channel blocked.
1988,
Pubmed Baumann,
Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems.
1988,
Pubmed Bennett,
Catecholamines modulate the delayed rectifying potassium current (IK) in guinea pig ventricular myocytes.
1987,
Pubmed Boyle,
Hormonal regulation of K+-channel messenger RNA in rat myometrium during oestrus cycle and in pregnancy.
,
Pubmed
,
Xenbase Boyle,
Xenopus oocytes injected with rat uterine RNA express very slowly activating potassium currents.
1987,
Pubmed
,
Xenbase Butler,
A family of putative potassium channel genes in Drosophila.
1989,
Pubmed Dash,
Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
1987,
Pubmed
,
Xenbase Dunn,
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
1983,
Pubmed Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase Giles,
Voltage clamp of bull-frog cardiac pace-maker cells: a quantitative analysis of potassium currents.
1985,
Pubmed Hubbard,
Synthesis and processing of asparagine-linked oligosaccharides.
1981,
Pubmed Iverson,
A-type potassium channels expressed from Shaker locus cDNA.
1988,
Pubmed
,
Xenbase Jan,
Voltage-sensitive ion channels.
1989,
Pubmed Kamb,
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.
1988,
Pubmed Kass,
Block of outward current in cardiac Purkinje fibers by injection of quaternary ammonium ions.
1982,
Pubmed Krebs,
Phosphorylation-dephosphorylation of enzymes.
1979,
Pubmed Krieg,
In vitro RNA synthesis with SP6 RNA polymerase.
1987,
Pubmed Lotan,
Adenosine-induced slow ionic currents in the Xenopus oocyte.
1982,
Pubmed
,
Xenbase Lotan,
Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA.
1989,
Pubmed
,
Xenbase Matsuura,
An analysis of the delayed outward current in single ventricular cells of the guinea-pig.
1987,
Pubmed Murai,
Molecular cloning and sequence analysis of human genomic DNA encoding a novel membrane protein which exhibits a slowly activating potassium channel activity.
1989,
Pubmed
,
Xenbase Newman,
Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction.
1988,
Pubmed Nielsen,
Preparation of capped RNA transcripts using T7 RNA polymerase.
1986,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schwarz,
Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila.
1988,
Pubmed Sippel,
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
1973,
Pubmed Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase Takumi,
Cloning of a membrane protein that induces a slow voltage-gated potassium current.
1988,
Pubmed
,
Xenbase Timpe,
Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Tindall,
Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.
1988,
Pubmed Tohse,
Intracellular Ca2+ and protein kinase C modulate K+ current in guinea pig heart cells.
1987,
Pubmed Walsh,
Regulation of a heart potassium channel by protein kinase A and C.
1988,
Pubmed Woodgett,
Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements.
1986,
Pubmed
