Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Domains of U1 snRNA which are functionally important have been identified using a splicing complementation assay in Xenopus oocytes. Mutations in, and deletions of, all three of the hairpin loop structures near the 5' end of the RNA are strongly deleterious. Similarly, mutation of the Sm binding site abolishes complementation activity. Analysis of the protein binding properties of the mutant U1 snRNAs reveals that three of the functionally important domains, the first two hairpin loops and the Sm binding site, are required for interaction with U1 snRNP proteins. The fourth functionally important domain does not detectably affect snRNP protein binding and is not evolutionarily conserved. All of the deleterious mutations are shown to have similar effects on in vivo splicing complex formation.
Bach,
20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.
1989, Pubmed
Bach,
20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.
1989,
Pubmed Billings,
Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.
1982,
Pubmed Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed Blencowe,
Antisense probing of the human U4/U6 snRNP with biotinylated 2'-OMe RNA oligonucleotides.
1989,
Pubmed Chabot,
Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes.
1987,
Pubmed Epstein,
Site-specific cleavage by T1 RNase of U-1 RNA in u-1 ribonucleoprotein particles.
1981,
Pubmed Frendewey,
Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.
1985,
Pubmed Guthrie,
Spliceosomal snRNAs.
1988,
Pubmed Habets,
Further characterization and subcellular localization of Sm and U1 ribonucleoprotein antigens.
1985,
Pubmed Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase Hamm,
Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.
1988,
Pubmed
,
Xenbase Hamm,
In vitro assembly of U1 snRNPs.
1987,
Pubmed
,
Xenbase Hernandez,
Formation of the 3' end of U1 snRNA requires compatible snRNA promoter elements.
1986,
Pubmed Kiss,
Plant small nuclear RNAs. III. The complete primary and secondary structure of broad bean U2 RNA: phylogenetic and functional implications.
1987,
Pubmed Konarska,
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.
1986,
Pubmed Konarska,
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.
1987,
Pubmed Kramer,
The gapped duplex DNA approach to oligonucleotide-directed mutation construction.
1984,
Pubmed Krämer,
Presplicing complex formation requires two proteins and U2 snRNP.
1988,
Pubmed Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed Lamond,
A mutational analysis of spliceosome assembly: evidence for splice site collaboration during spliceosome formation.
1987,
Pubmed Lamond,
Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2'-OMe RNA.
1989,
Pubmed Lamond,
Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.
1988,
Pubmed Legrain,
Early commitment of yeast pre-mRNA to the spliceosome pathway.
1988,
Pubmed Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase Liautard,
Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs.
1982,
Pubmed Lutz-Freyermuth,
The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.
1989,
Pubmed Mattaj,
Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs.
1986,
Pubmed
,
Xenbase Mount,
The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
1983,
Pubmed Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Pan,
U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing.
1989,
Pubmed
,
Xenbase Patton,
The Mr 70,000 protein of the U1 small nuclear ribonucleoprotein particle binds to the 5' stem-loop of U1 RNA and interacts with Sm domain proteins.
1988,
Pubmed Patton,
Reconstitution of the U1 small nuclear ribonucleoprotein particle.
1987,
Pubmed Patton,
U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins.
1989,
Pubmed Pikielny,
Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
,
Pubmed Query,
A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
1989,
Pubmed Ruby,
An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.
1988,
Pubmed Ruskin,
A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.
1988,
Pubmed Scherly,
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
1989,
Pubmed
,
Xenbase Seraphin,
Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing.
1989,
Pubmed Séraphin,
A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.
1988,
Pubmed Siliciano,
5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.
1988,
Pubmed Studier,
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
1986,
Pubmed Surowy,
Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.
1989,
Pubmed Tabor,
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
1985,
Pubmed Yuo,
Genetic analysis of the role of human U1 snRNA in mRNA splicing: I. Effect of mutations in the highly conserved stem-loop I of U1.
1989,
Pubmed Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
1984,
Pubmed
,
Xenbase Zhuang,
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.
1986,
Pubmed Zillmann,
U1 small nuclear ribonucleoproteins are required early during spliceosome assembly.
1987,
Pubmed
