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Mol Cell Biol
1990 Jan 01;101:333-40. doi: 10.1128/mcb.10.1.333-340.1990.
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Diacylglycerol production in Xenopus laevis oocytes after microinjection of p21ras proteins is a consequence of activation of phosphatidylcholine metabolism.
Lacal JC.
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Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and GVBD activity were dependent on the ability of the proteins to undergo membrane translocation. Alterations of DAG levels or GVBD activity did not correlate with changes in the levels of inositol phosphates. However, at minimal doses sufficient to achieve maximal biological response, a biphasic increase in the amounts of phosphocholine and CDP-choline was observed. The first burst of phosphocholine and CDP-choline preceded the increase in DAG levels. The second peak paralleled the appearance of DAG. Choline kinase activity was also increased in oocyte extracts after p21ras microinjection. These results suggest that both the synthesis and degradation of phosphatidylcholine are activated after microinjection of ras proteins into Xenopus oocytes, resulting in a net production of DAG.
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Inositol trisphosphate and diacylglycerol: two interacting second messengers.
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ras proteins can induce meiosis in Xenopus oocytes.
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Xenbase Buss,
Direct identification of palmitic acid as the lipid attached to p21ras.
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Pubmed Fleischman,
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All ras proteins are polyisoprenylated but only some are palmitoylated.
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Mutant but not normal p21 ras elevates inositol phospholipid breakdown in two different cell systems.
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Novel source of 1,2-diacylglycerol elevated in cells transformed by Ha-ras oncogene.
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Pubmed Lacal,
Transforming activity of ras proteins translocated to the plasma membrane by a myristoylation sequence from the src gene product.
1988,
Pubmed Lacal,
Rapid stimulation of diacylglycerol production in Xenopus oocytes by microinjection of H-ras p21.
1987,
Pubmed
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Involvement of functional protein kinase C in the mitogenic response to the H-ras oncogene product.
1987,
Pubmed Paddon,
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1982,
Pubmed Pelech,
Regulation of phosphatidylcholine biosynthesis.
1984,
Pubmed Preiss,
Quantitative measurement of sn-1,2-diacylglycerols present in platelets, hepatocytes, and ras- and sis-transformed normal rat kidney cells.
1986,
Pubmed Seuwen,
Deregulation of hamster fibroblast proliferation by mutated ras oncogenes is not mediated by constitutive activation of phosphoinositide-specific phospholipase C.
1988,
Pubmed Shih,
Identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus.
1982,
Pubmed Trahey,
A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants.
1987,
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Xenbase Ulane,
Evidence for the existence of a single enzyme catalyzing the phosphorylation of choline and ethanolamine in primate lung.
1978,
Pubmed Wakelam,
Normal p21N-ras couples bombesin and other growth factor receptors to inositol phosphate production.
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Pubmed Walker,
Phosphatidylinositol kinase is activated in membranes derived from cells treated with epidermal growth factor.
1987,
Pubmed Willumsen,
Harvey murine sarcoma virus p21 ras protein: biological and biochemical significance of the cysteine nearest the carboxy terminus.
1984,
Pubmed Willumsen,
The p21 ras C-terminus is required for transformation and membrane association.
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Pubmed Wolfman,
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