Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Treatment with insulin or progesterone or microinjection of the transforming protein product of Ha-rasVal-12,Thr-59 (p21) is known to induce germinal vesicle breakdown in Xenopus oocytes. We have investigated the effect of p21 on S6 kinase and the H1 histone kinase of maturation-promoting factor in the presence and absence of antisense oligonucleotides against the c-mosxe proto-oncogene. Injection of p21 led to a rapid increase in S6 phosphorylation, with kinetics similar to those previously observed with insulin. Microinjection of c-mosxe antisense oligonucleotides inhibited germinal vesicle breakdown induced by p21 and totally abolished S6 kinase activation by insulin or progesterone but only partially inhibited activation by p21. However, the activation of p34cdc2 protein kinase by all three stimuli was blocked by antisense oligonucleotides. The results suggest that in oocyte maturation c-mosxe functions downstream of p21 but upstream of p34cdc2 and S6 kinase activation, although not all p21-induced events require c-mosxe.
Allende,
Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors.
1988, Pubmed,
Xenbase
Allende,
Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors.
1988,
Pubmed
,
Xenbase Birchmeier,
ras proteins can induce meiosis in Xenopus oocytes.
1985,
Pubmed
,
Xenbase Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Deshpande,
Insulin induction of Xenopus laevis oocyte maturation is inhibited by monoclonal antibody against p21 ras proteins.
1987,
Pubmed
,
Xenbase Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase El-Etr,
Meiotic maturation in Xenopus laevis oocytes initiated by insulin.
1979,
Pubmed
,
Xenbase Erikson,
Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
1987,
Pubmed
,
Xenbase Erikson,
A protein kinase from Xenopus eggs specific for ribosomal protein S6.
1985,
Pubmed
,
Xenbase Erikson,
Purification and characterization of a protein kinase from Xenopus eggs highly specific for ribosomal protein S6.
1986,
Pubmed
,
Xenbase Finidori-Lepicard,
Progesterone inhibits membrane-bound adenylate cyclase in Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase Gibbs,
Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.
1984,
Pubmed Glass,
Isolation of phosphorylated peptides and proteins on ion exchange papers.
1978,
Pubmed Goldman,
Expression of c-mos RNA in germ cells of male and female mice.
1987,
Pubmed Hanocq-Quertier,
Phosphorylation of ribosomal proteins during maturation of Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase Keshet,
Developmental regulation of ovarian-specific Mos expression.
1988,
Pubmed Kishimoto,
Generality of the action of various maturation-promoting factors.
1982,
Pubmed
,
Xenbase Korn,
Ras p21 as a potential mediator of insulin action in Xenopus oocytes.
1987,
Pubmed
,
Xenbase Kruppa,
The phosphorylation of ribosomal protein S6 from progesterone-stimulated Xenopus laevis oocytes. Kinetic studies and phosphopeptide analysis.
1983,
Pubmed
,
Xenbase Lacal,
Rapid stimulation of diacylglycerol production in Xenopus oocytes by microinjection of H-ras p21.
1987,
Pubmed
,
Xenbase Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase Maller,
Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase Maller,
Early effect of progesterone on levels of cyclic adenosine 3':5'-monophosphate in Xenopus oocytes.
1979,
Pubmed
,
Xenbase Maxwell,
Serine kinase activity associated with Maloney murine sarcoma virus-124-encoded p37mos.
1985,
Pubmed Mutter,
Distinct developmental patterns of c-mos protooncogene expression in female and male mouse germ cells.
1987,
Pubmed Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase Noda,
Flat revertants isolated from Kirsten sarcoma virus-transformed cells are resistant to the action of specific oncogenes.
1983,
Pubmed Propst,
c-mos proto-oncogene RNA transcripts in mouse tissues: structural features, developmental regulation, and localization in specific cell types.
1987,
Pubmed Ray,
Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.
1988,
Pubmed Reynhout,
Studies on the appearance and nature of a maturation-inducing factor in the cytoplasm of amphibian oocytes exposed to progesterone.
1974,
Pubmed
,
Xenbase Sadler,
A similar pool of cyclic AMP phosphodiesterase in Xenopus oocytes is stimulated by insulin, insulin-like growth factor 1, and [Val12,Thr59]Ha-ras protein.
1989,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase Smith,
Requirement for c-ras proteins during viral oncogene transformation.
,
Pubmed Stefanovic,
Post-transcriptional regulation by insulin of Xenopus ribosomal protein S6 kinase.
1988,
Pubmed
,
Xenbase Stith,
Induction of meiotic maturation in Xenopus oocytes by 12-O-tetradecanoylphorbol 13-acetate.
1987,
Pubmed
,
Xenbase Stith,
The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase Tachibana,
Preliminary characterization of maturation-promoting factor from yeast Saccharomyces cerevisiae.
1987,
Pubmed
,
Xenbase Wasserman,
Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytes.
1982,
Pubmed
,
Xenbase