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Linear DNA injected into Xenopus laevis oocyte nuclei recombines with high efficiency if homologous sequences are present at overlapping molecular ends. We found that injected linear DNA was degraded by a 5'----3' strand-specific exonuclease activity during incubation in the oocyte nucleus to leave a heterogeneous population of 3'-tailed molecules. Decreasing the concentration of DNA injected increased the heterogeneity and the average rate of degradation. The 3' tails created were relatively stable; among molecules persisting after overnight incubation, many had 3' tails intact to within 10 bases of the original ends. DNA molecules that were efficient substrates for homologous recombination in oocytes were also partially degraded, leaving 3' tails. We found no evidence for other potent nuclease activities. If molecules with recessed 3'-OH ends were injected, endogenous polymerase efficiently resynthesized complementary strands before degradation of the 5' tails occurred. 3'-tailed molecules are plausible intermediates in the initiation of homologous recombination events in Xenopus oocyte nuclei.
Burgers,
Exonuclease V from Saccharomyces cerevisiae. A 5'----3'-deoxyribonuclease that produces dinucleotides in a sequential fashion.
1988, Pubmed
Burgers,
Exonuclease V from Saccharomyces cerevisiae. A 5'----3'-deoxyribonuclease that produces dinucleotides in a sequential fashion.
1988,
Pubmed Carroll,
Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase Carroll,
Genetic recombination of bacteriophage lambda DNAs in Xenopus oocytes.
1983,
Pubmed
,
Xenbase Church,
Genomic sequencing.
1984,
Pubmed Clewell,
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
1969,
Pubmed Cortese,
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
1980,
Pubmed
,
Xenbase Craigie,
Mechanism of transposition of bacteriophage Mu: structure of a transposition intermediate.
1985,
Pubmed Doherty,
Genetic recombination of bacterial plasmid DNA. Physical and genetic analysis of the products of plasmid recombination in Escherichia coli.
1983,
Pubmed Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Ganea,
Characterization of an ATP-dependent DNA strand transferase from human cells.
1987,
Pubmed Grzesiuk,
Recombination of DNAs in Xenopus oocytes based on short homologous overlaps.
1987,
Pubmed
,
Xenbase Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed Haber,
Physical monitoring of meiotic and mitotic recombination in yeast.
1988,
Pubmed Hsieh,
Partial purification and characterization of a recombinase from human cells.
1986,
Pubmed Kmiec,
Heteroduplex formation and polarity during strand transfer promoted by Ustilago rec 1 protein.
1983,
Pubmed Kolodner,
Purification and characterization of an activity from Saccharomyces cerevisiae that catalyzes homologous pairing and strand exchange.
1987,
Pubmed Laskey,
Induction of chromosome replication during maturation of amphibian oocytes.
1983,
Pubmed
,
Xenbase Legerski,
Repair of UV-induced lesions in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase Lin,
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
1984,
Pubmed McCarthy,
Sensitive homologous recombination strand-transfer assay: partial purification of a Drosophila melanogaster enzyme and detection of sequence effects on the strand-transfer activity of RecA protein.
1988,
Pubmed Mertz,
Linear DNA does not form chromatin containing regularly spaced nucleosomes.
1982,
Pubmed
,
Xenbase Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
1982,
Pubmed
,
Xenbase Pfeiffer,
Joining of nonhomologous DNA double strand breaks in vitro.
1988,
Pubmed
,
Xenbase Randahl,
DNA-repair reactions by purified HeLa DNA polymerases and exonucleases.
1988,
Pubmed Resnick,
The repair of double-strand breaks in DNA; a model involving recombination.
1976,
Pubmed Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase Stahl,
Roles of double-strand breaks in generalized genetic recombination.
1986,
Pubmed Stephens,
Easy-to-use equipment for the accurate microinjection of nanoliter volumes into the nuclei of amphibian oocytes.
1981,
Pubmed
,
Xenbase Sun,
Double-strand breaks at an initiation site for meiotic gene conversion.
1989,
Pubmed Sutcliffe,
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
1979,
Pubmed Szostak,
The double-strand-break repair model for recombination.
1983,
Pubmed Thaler,
DNA double-chain breaks in recombination of phage lambda and of yeast.
1988,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Wu,
Formation of nascent heteroduplex structures by RecA protein and DNA.
1982,
Pubmed Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
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