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We have isolated multiple independent cDNA clones that represent mRNA sequences of immunoglobulin heavy chains from the spleen of adult Xenopus laevis. These cDNA clones contained constant (C) region sequences that were either Cmu or a separate C region sequence believed to be Cnu. In individual cDNA clones the C region sequences were associated with independent heavy-chain variable region (VH) sequences that were classifiable into five distinct families. Genomic Southern blotting analyses with family-specific probes indicated that the haploid genome contains a minimum of 80 VH gene segments, a number similar to that found in various mammalian species. Multiple JH and putative DH segments were also identified (J, joining; D, diversity). Analyses of 13 independent VHDJH junctions suggest that combinatorial and junctional diversification mechanisms probably arose early in vertebrate evolution. Finally, comparison of Xenopus VH sequences to those from other vertebrates indicated conservation of V region framework residues that are responsible for the tertiary structure of the Fv throughout evolution.
Alt,
Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
1982, Pubmed
Alt,
Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.
1982,
Pubmed Berman,
Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.
1988,
Pubmed Brandt,
Antibody diversity in amphibians: evidence for the inheritance of idiotypic specificities in isogenic Xenopus.
1980,
Pubmed
,
Xenbase Brodeur,
The immunoglobulin heavy chain variable region (Igh-V) locus in the mouse. I. One hundred Igh-V genes comprise seven families of homologous genes.
1984,
Pubmed Capra,
Phylogenetically associated residues within the VH3 subgroup of several mammalian species. Evidence for a "pauci-gene" basis for antibody diversity.
1973,
Pubmed Chien,
T-cell receptor delta gene rearrangements in early thymocytes.
,
Pubmed Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed Chothia,
Domain association in immunoglobulin molecules. The packing of variable domains.
1985,
Pubmed Diener,
Phylogenetic studies on the immune response. I. Localization of antigens and immune response in the toad, Bufo marinus.
1966,
Pubmed Du Pasquier,
Antibody diversity in lower vertebrates--why is it so restricted?
1982,
Pubmed Du Pasquier,
Antibody diversity in amphibians: inheritance of isoelectric focusing antibody patterns in isogenic frogs.
1978,
Pubmed
,
Xenbase Hadji-Azimi,
Studies on Xenopus laevis immunoglobulins.
1971,
Pubmed
,
Xenbase Haire,
A second Xenopus immunoglobulin heavy chain constant region isotype gene.
1989,
Pubmed
,
Xenbase Hsu,
A third immunoglobulin class in amphibians.
1985,
Pubmed
,
Xenbase Hsu,
Studies on Xenopus immunoglobulins using monoclonal antibodies.
1984,
Pubmed
,
Xenbase Kabat,
Evidence supporting somatic assembly of the DNA segments (minigenes), coding for the framework, and complementarity-determining segments of immunoglobulin variable regions.
1979,
Pubmed Kabat,
Attempts to locate complementarity-determining residues in the variable positions of light and heavy chains.
1971,
Pubmed Kokubu,
Diverse organization of immunoglobulin VH gene loci in a primitive vertebrate.
1988,
Pubmed Kraft,
Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.
1988,
Pubmed Kurosawa,
Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.
1982,
Pubmed Litman,
Complete nucleotide sequences of three VH genes in Caiman, a phylogenetically ancient reptile: evolutionary diversification in coding segments and variation in the structure and organization of recombination elements.
1985,
Pubmed Mäkelä,
Lack of heterogeneity in antihapten antibodies of a phylogenetically primitive shark.
1980,
Pubmed Marchalonis,
Phylogenetic origins of antibody structure. II. Immunoglobulins in the primary immune response of the bullfrog, Rana catesbiana.
1966,
Pubmed Novotný,
Different degrees of interspecies homology in immunoglobulin lamda chain constant domain correlated with three-dimensional structure.
1975,
Pubmed Ravetch,
Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.
1981,
Pubmed Reynaud,
A hyperconversion mechanism generates the chicken light chain preimmune repertoire.
1987,
Pubmed Sakano,
Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
1980,
Pubmed Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Schwager,
Organization and rearrangement of immunoglobulin M genes in the amphibian Xenopus.
1988,
Pubmed
,
Xenbase Schwager,
Amino acid sequence of heavy chain from Xenopus laevis IgM deduced from cDNA sequence: implications for evolution of immunoglobulin domains.
1988,
Pubmed
,
Xenbase Tonegawa,
Somatic generation of antibody diversity.
1983,
Pubmed Turner,
Thymic dependence of amphibian antibody responses.
1974,
Pubmed
,
Xenbase Wabl,
Antibody patterns in genetically identical frogs.
1976,
Pubmed
,
Xenbase Wu,
An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity.
1970,
Pubmed Yamawaki-Kataoka,
Nucleotide sequences of variable region segments of the immunoglobulin heavy chain of Xenopus laevis.
1987,
Pubmed
,
Xenbase Yancopoulos,
Developmentally controlled and tissue-specific expression of unrearranged VH gene segments.
1985,
Pubmed
