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Protein import through the nuclear pore complex is a multistep process.
Akey CW, Goldfarb DS.
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The transport of macromolecules across the nuclear envelope is mediated by the nuclear pore complex (NPC). Using cryo-electron microscopy and image processing we have mapped the interaction of three specific gold probes with the NPC and obtained projection maps of two possible intermediates in nuclear import. The probes used in these experiments were (a) mAb-414, which cross-reacts with Xenopus nucleoporins containing O-linked N-acetyl glucosamines; (b) wheat germ agglutinin, a transport inhibitor; and (c) nucleoplasmin, a transport substrate. Strong binding sites of the three probes are circularly arrayed on NPCs between radii of 100 and 125 A and may be coextensive. These results suggest that nucleoplasmin-gold (NP-gold) can form at least three distinct complexes with a central transport assembly of the NPC, which may represent intermediates of a multistep protein import pathway. Initially, NP-gold appears to bind at multiple sites located around the periphery of the closed NPC transporter and also directly over the center where it can dock. In a subsequent step NP-gold is translocated through the nuclear pore.
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989, Pubmed
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989,
Pubmed Akey,
Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy.
1989,
Pubmed
,
Xenbase Baglia,
Nuclear ribonucleoprotein release and nucleoside triphosphatase activity are inhibited by antibodies directed against one nuclear matrix glycoprotein.
1983,
Pubmed Davis,
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.
1987,
Pubmed Davis,
Identification and characterization of a nuclear pore complex protein.
1986,
Pubmed Dingwall,
Protein import into the cell nucleus.
1986,
Pubmed Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase Dingwall,
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
1988,
Pubmed Dubochet,
Cryo-electron microscopy of vitrified specimens.
1988,
Pubmed Dworetzky,
Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
1988,
Pubmed
,
Xenbase Dworetzky,
The effects of variations in the number and sequence of targeting signals on nuclear uptake.
1988,
Pubmed
,
Xenbase Featherstone,
A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
1988,
Pubmed
,
Xenbase Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
,
Xenbase Franke,
Structure, biochemistry, and functions of the nuclear envelope.
1974,
Pubmed Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Hanover,
O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins.
1987,
Pubmed Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed Lanford,
Effect of basic and nonbasic amino acid substitutions on transport induced by simian virus 40 T-antigen synthetic peptide nuclear transport signals.
1988,
Pubmed Milligan,
Molecular structure determination of crystalline specimens in frozen aqueous solutions.
1984,
Pubmed Moreland,
Amino acid sequences that determine the nuclear localization of yeast histone 2B.
1987,
Pubmed Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase Newport,
The nucleus: structure, function, and dynamics.
1987,
Pubmed Paine,
Nuclear envelope permeability.
1975,
Pubmed Peters,
Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility.
1986,
Pubmed Richardson,
Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
1988,
Pubmed
,
Xenbase Rihs,
Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40 T-antigen.
1989,
Pubmed Smith,
The nuclear location signal.
1985,
Pubmed Snow,
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.
1987,
Pubmed
,
Xenbase Stevens,
RNA transport from nucleus to cytoplasm in Chironomus salivary glands.
1966,
Pubmed Tobian,
tRNA nuclear transport: defining the critical regions of human tRNAimet by point mutagenesis.
1985,
Pubmed
,
Xenbase Unwin,
A large particle associated with the perimeter of the nuclear pore complex.
1982,
Pubmed
,
Xenbase Unwin,
Molecular structure determination by electron microscopy of unstained crystalline specimens.
1975,
Pubmed Wright,
The crystal structure of wheat germ agglutinin at 2-2 A resolution.
1977,
Pubmed Yoneda,
Reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells.
1987,
Pubmed
,
Xenbase Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
