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Oligonucleotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snRNPs.
Pan ZQ, Ge H, Fu XY, Manley JL, Prives C.
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We have investigated the roles of U1 and U2 snRNP particles in SV40 pre-mRNA splicing by oligonucleotide-targeted degradation of U1 or U2 snRNAs in Xenopus laevis oocytes. Microinjection of oligonucleotides complementary to regions of U1 or U2 RNAs either in the presence or absence of SV40 DNA resulted in specific cleavage of the corresponding snRNA. Unexpectedly, degradation of U1 or U2 snRNA was far more extensive when the oligonucleotide was injected without, or prior to, introduction of viral DNA. In either co-injected or pre-injected oocytes, these oligonucleotides caused a dramatic reduction in the accumulation of spliced SV40 mRNA expressed from the viral late region, and a commensurate increase in unspliced late RNA. When pre-injected, two different U2 specific oligonucleotides also inhibited the formation of both large and small tumor antigen spliced early mRNAs. However, even when, by pre-injection of a U1 5' end-specific oligonucleotide, greater than 95% degradation of the U1 snRNA 5' ends occurred in oocytes, no reduction in early pre-mRNA splicing was observed. In contrast, the same U1 5' end oligonucleotide, when added to HeLa splicing extracts, substantially inhibited the splicing of SV40 early pre-mRNA, indicating that U1 mRNP is not totally dispensable for early splicing. These findings confirm and extend our earlier observations which suggested that different pre-mRNAs vary in their requirements for snRNPs.
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2550896 ???displayArticle.pmcLink???PMC318349 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986, Pubmed
Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986,
Pubmed Bindereif,
An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.
1987,
Pubmed Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed Black,
Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.
1986,
Pubmed Cazenave,
Rate of degradation of [alpha]- and [beta]-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies.
1987,
Pubmed
,
Xenbase Chabot,
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
1985,
Pubmed Fakan,
Ultrastructural distribution of nuclear ribonucleoproteins as visualized by immunocytochemistry on thin sections.
1984,
Pubmed Forbes,
Small nuclear RNA transcription and ribonucleoprotein assembly in early Xenopus development.
1983,
Pubmed
,
Xenbase Forbes,
Differential expression of multiple U1 small nuclear RNAs in oocytes and embryos of Xenopus laevis.
1984,
Pubmed
,
Xenbase Fradin,
Splicing pathways of SV40 mRNAs in X. laevis oocytes differ in their requirements for snRNPs.
1984,
Pubmed
,
Xenbase Fu,
Factors influencing alternative splice site utilization in vivo.
1987,
Pubmed Good,
Both VP2 and VP3 are synthesized from each of the alternative spliced late 19S RNA species of simian virus 40.
1988,
Pubmed Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
,
Xenbase Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase Krainer,
Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
1985,
Pubmed Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed Lund,
True genes for human U1 small nuclear RNA. Copy number, polymorphism, and methylation.
1984,
Pubmed Maniatis,
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing.
,
Pubmed Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase Mertz,
Linear DNA does not form chromatin containing regularly spaced nucleosomes.
1982,
Pubmed
,
Xenbase Michaeli,
Regulation of simian virus 40 gene expression in Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase Michaeli,
An excised SV40 intron accumulates and is stable in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Michaeli,
pBR322 DNA inhibits simian virus 40 gene expression in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase Miller,
Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA.
1982,
Pubmed
,
Xenbase Mottram,
Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. brucei: identification of the U2, U4, and U6 RNA analogs.
1989,
Pubmed Mount,
A catalogue of splice junction sequences.
1982,
Pubmed Mount,
The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
1983,
Pubmed Noble,
Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sites.
1987,
Pubmed
,
Xenbase Noble,
In vitro splicing of simian virus 40 early pre mRNA.
1986,
Pubmed Padgett,
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
1983,
Pubmed Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Parker,
Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA.
1987,
Pubmed Peebles,
A self-splicing RNA excises an intron lariat.
1986,
Pubmed Ruby,
An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.
1988,
Pubmed Ruskin,
A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.
1988,
Pubmed Sharp,
Splicing of messenger RNA precursors.
1987,
Pubmed van der Krol,
Modulation of eukaryotic gene expression by complementary RNA or DNA sequences.
1988,
Pubmed van der Veen,
Excised group II introns in yeast mitochondria are lariats and can be formed by self-splicing in vitro.
1986,
Pubmed Westin,
Human U2 and U1 RNA genes use similar transcription signals.
1984,
Pubmed
,
Xenbase Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase Zhuang,
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.
1986,
Pubmed
