Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1989 Aug 01;88:2343-51. doi: 10.1002/j.1460-2075.1989.tb08362.x.
Show Gene links
Show Anatomy links
Nucleosomes inhibit both transcriptional initiation and elongation by RNA polymerase III in vitro.
Morse RH.
???displayArticle.abstract???
To examine the effect of nucleosomes on in vitro transcription, purified chicken erythrocyte core histones and plasmid DNA bearing the Xenopus 5S RNA gene were assembled into nucleosomes and used as templates for transcription in a Xenopus oocyte nuclear extract. Plasmids having a nucleosome incorporating a specific region of the gene were selected by treating the reconstituted molecules with restriction endonucleases. In this way, it was shown that a nucleosome on or close to the internal control region of the 5S RNA gene inhibits transcription. Furthermore, experiments with 5S maxigenes showed that RNA polymerase III, in contrast to SP6 RNA polymerase, will not transcribe through a nucleosome in vitro.
Amero,
The characterization of ribosomal RNA gene chromatin from Physarum polycephalum.
1988, Pubmed
Amero,
The characterization of ribosomal RNA gene chromatin from Physarum polycephalum.
1988,
Pubmed Benyajati,
Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.
1976,
Pubmed Bina-Stein,
H5 Histone and DNA-relaxing enzyme of chicken erythrocytes. Interaction with superhelical DNA.
1976,
Pubmed Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase Church,
Genomic sequencing.
1984,
Pubmed Das,
Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III.
1988,
Pubmed
,
Xenbase De Bernardin,
Structure of in-vivo transcribing chromatin as studied in simian virus 40 minichromosomes.
1986,
Pubmed Geiduschek,
Transcription by RNA polymerase III.
1988,
Pubmed Germond,
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
1975,
Pubmed Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase Hartley,
Cloning multiple copies of a DNA segment.
1981,
Pubmed Keller,
Biochemical and ultrastructural analysis of SV40 chromatin.
1978,
Pubmed Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase LaFlamme,
Xenopus endo B is a keratin preferentially expressed in the embryonic notochord.
1988,
Pubmed
,
Xenbase Lassar,
Stable transcription complex on a class III gene in a minichromosome.
1985,
Pubmed
,
Xenbase Liu,
Supercoiling of the DNA template during transcription.
1987,
Pubmed Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed Losa,
A bacteriophage RNA polymerase transcribes in vitro through a nucleosome core without displacing it.
1987,
Pubmed
,
Xenbase Matsui,
Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly.
1987,
Pubmed Meneguzzi,
Transcription of nucleosomal DNA in SV40 minichromosomes by eukaryotic and prokaryotic RNA polymerases.
1979,
Pubmed Morse,
Effect of trypsinization and histone H5 addition on DNA twist and topology in reconstituted minichromosomes.
1986,
Pubmed Morse,
DNA in the nucleosome.
1988,
Pubmed Murphy,
The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.
1987,
Pubmed Poljak,
Competition for formation of nucleosomes on fragmented SV40 DNA: a hyperstable nucleosome forms on the termination region.
1987,
Pubmed Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase Schlissel,
The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
1984,
Pubmed
,
Xenbase Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed Stein,
A model chromatin assembly system. Factors affecting nucleosome spacing.
1984,
Pubmed Stein,
Unique positioning of reconstituted nucleosomes occurs in one region of simian virus 40 DNA.
1987,
Pubmed Wasylyk,
Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro.
1979,
Pubmed Wasylyk,
Transcription of in vitro assembled chromatin by Escherichia coli RNA polymerase.
1979,
Pubmed Weisbrod,
Active chromatin of oocytes injected with somatic cell nuclei or cloned DNA.
1982,
Pubmed
,
Xenbase Williamson,
Transcription of histone-covered T7 DNA by Escherichia coli RNA polymerase.
1978,
Pubmed Wolffe,
Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1.
1989,
Pubmed
,
Xenbase Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase Zimmerman,
Do histones bind to a specific group of DNA sequences in chromatin? A test based on DNA ligase action on reconstituted chromatin.
1975,
Pubmed
