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Presence of multiple species of polypeptides immunologically related to transcription factor TFIIIA in adult Xenopus tissues.
Yasui W, Ryoji M.
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Transcription of 5S RNA gene in Xenopus oocytes requires a 38 kDa transcription factor TFIIIA, which interacts with the 50 bp internal control region of the gene. We looked for TFIIIA-like polypeptides in the extracts of adult Xenopus tissues on the basis of their antigenic cross-reactivity to anti-TFIIIA antibody. Several species of polypeptides ranging from 30 to 50 kDa were found in kidney, stomach, liver and testis. Although these polypeptides reacted specifically to anti-TFIIIA antibody, proteolytic peptide mapping of three representative ones did not reveal any mutual similarities. They also seemed to be distinct from TFIIIA. Possible functions of these proteins are discussed.
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2762150 ???displayArticle.pmcLink???PMC318182 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
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