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Pathways of nucleoprotein assembly on 5S RNA genes in a Xenopus oocyte S-150 extract.
Razik MA, Blanco J, Gottesfeld JM.
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Conditions for transcription and nucleosome assembly of plasmids bearing Xenopus 5S RNA genes have been monitored in the whole oocyte S-150 extract (1). We find that the optimal conditions for transcription differ substantially from optimal conditions for nucleosome assembly. DNA molecules bearing as few as 50% of the native density of nucleosomes are transcriptionally inert. Although the 5S gene-specific transcription factor TFIIIA is in excess in this extract, these nucleosome reconstitutes do not exhibit TFIIA-like DNase footprints nor do these reconstitutes bind exogenous TFIIIA. We have also examined the nucleotide requirement for DNA supercoiling and for generation of 5S gene transcription complexes. Supercoiling associated with nucleosome assembly does not require ATP; however, nucleotide hydrolysis is required for establishment of active complexes. Phosphorylation of a 200 kdalton protein occurs in a 5S DNA-dependent manner concurrent with the generation of primed transcription complexes. Results of nondenaturing gel electrophoresis coupled with a second dimension of SDS gel electrophoresis suggest that the 200 kD protein may be a component of the 5S RNA gene transcription complex.
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2544856 ???displayArticle.pmcLink???PMC317923 ???displayArticle.link???Nucleic Acids Res ???displayArticle.grants???[+]
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985, Pubmed,
Xenbase
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
,
Xenbase Bieker,
Characterization of the nucleotide requirement for elimination of the rate-limiting step in 5 S RNA gene transcription.
1986,
Pubmed Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase Chen,
Nonintercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II.
1984,
Pubmed Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed Garner,
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
1981,
Pubmed Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase Gottesfeld,
Novobiocin inhibits RNA polymerase III transcription in vitro by a mechanism distinct from DNA topoisomerase II.
1986,
Pubmed Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase Gottesfeld,
DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis.
1987,
Pubmed
,
Xenbase Hoeffler,
Activation of transcription factor IIIC by the adenovirus E1A protein.
1988,
Pubmed Hsiang,
Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I.
1985,
Pubmed Kmiec,
Gyration is required for 5S RNA transcription from a chromatin template.
1986,
Pubmed
,
Xenbase Kmiec,
The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts.
1985,
Pubmed
,
Xenbase Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase Korn,
Transcription of Xenopus 5S ribosomal RNA genes.
1982,
Pubmed
,
Xenbase Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase Ladiges,
The canine major histocompatibility complex. Supertypic specificities defined by the primed lymphocyte test (PLT).
1984,
Pubmed Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed Matsui,
Transcription of adenovirus 2 major late and peptide IX genes under conditions of in vitro nucleosome assembly.
1987,
Pubmed Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase Millstein,
Differential transcription of Xenopus oocyte and somatic-type 5 S genes in a Xenopus oocyte extract.
1987,
Pubmed
,
Xenbase Peck,
Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.
1987,
Pubmed
,
Xenbase Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase Schlissel,
The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
1984,
Pubmed
,
Xenbase Setzer,
Formation and stability of the 5 S RNA transcription complex.
1985,
Pubmed
,
Xenbase Shastry,
Multiple factors involved in the transcription of class III genes in Xenopus laevis.
1982,
Pubmed
,
Xenbase Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed Tso,
Structure of the gene for Xenopus transcription factor TFIIIA.
1986,
Pubmed
,
Xenbase Wolffe,
Differential 5S RNA gene expression in vitro.
1987,
Pubmed
,
Xenbase Wolffe,
Negative supercoiling is not required for 5S RNA transcription in vitro.
1987,
Pubmed
,
Xenbase Wolffe,
DNA replication in vitro erases a Xenopus 5S RNA gene transcription complex.
1986,
Pubmed
,
Xenbase Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
